Restriction map for
pFN21AE2285
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AfaI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 190 GTCGAC GT^[AC][GT]AC
AcyI 364 GACGCC G[GA]^CG[CT]C
AflII 331 CTTAAG C^TTAAG
ApaI 311 GGGCCC GGGCC^C
AspHI 106 GTGCTC G[AT]GC[AT]^C
AvaI 316 CCCGAG C^[CT]CG[GA]G
AvaII 432 GGACC G^G[AT]CC
BanII 311 GGGCCC G[GA]GC[CT]^C
BbsI 66 GTCTTC GAAGAC(2/6)
BfrI 331 CTTAAG C^TTAAG
BpuAI 66 GTCTTC GAAGAC(2/6)
BsaHI 364 GACGCC G[GA]^CG[CT]C
BsaI 268 GAGACC GGTCTC(1/5)
BsaWI 7 TCCGGT [AT]^CCGG[AT]
BsgI 438 CTGCAC GTGCAG(16/14)
BsiHKAI 106 GTGCTC G[AT]GC[AT]^C
BsoBI 316 CCCGAG C^[CT]CG[GA]G
BstAPI 396 GCATGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 141 CATCC GGATG(2/0)
DdeI 118 CTGAG C^T[ACGT]AG
DraII 311 GGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 109 CTCTTC CTCTTC(1/4)
EcoO109I 311 GGGCCCC [GA]G^G[ACGT]CC[CT]
FokI 141 CATCC GGATG(9/13)
HapII 8 CCGG C^CGG
HgaI 364 GACGC GACGC(5/10)
Hin1I 364 GACGCC G[GA]^CG[CT]C
HincII 190 GTCGAC GT[CT]^[GA]AC
HindII 190 GTCGAC GT[CT]^[GA]AC
HinfI 319 GAGTC G^A[ACGT]TC
HpaII 8 CCGG C^CGG
HphI 84 GGTGA GGTGA(8/7)
Ksp632I 109 CTCTTC CTCTTC(1/4)
MseI 332 TTAA T^TAA
MspA1I 435 CCGCTG C[AC]G^C[GT]G
MspI 8 CCGG C^CGG
PleI 319 GAGTC GAGTC(4/5)
SalI 190 GTCGAC G^TCGAC
SapI 108 GCTCTTC GCTCTTC(1/4)
SmlI 331 CTTAAG C^T[CT][GA]AG
TaqI 191 TCGA T^CGA
Tru9I 332 TTAA T^TAA
Tsp509I 401 AATT ^AATT
TspRI 465 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 191 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbvI 222 GCTGC GCAGC(8/12)
437 GCTGC
BmyI 106 GTGCTC G[AGT]GC[ACT]^C
311 GGGCCC
BpmI 145 CTGGAG CTGGAG(16/14)
481 CTGGAG
BsiYI 117 CCTGAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
144 CCTGGAGGAGG
BslI 117 CCTGAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
144 CCTGGAGGAGG
BsmAI 268 GAGAC GTCTC(1/5)
19 GTCTC
Bsp1286I 106 GTGCTC G[AGT]GC[ACT]^C
311 GGGCCC
BstYI 161 AGATCC [GA]^GATC[CT]
291 GGATCT
CfoI 93 GCGC GCG^C
325 GCGC
EcoNI 117 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
144 CCTGGAGGAGG
EcoT14I 272 CCAAGG C^C[AT][AT]GG
359 CCAAGG
HhaI 93 GCGC GCG^C
325 GCGC
HinP1I 93 GCGC G^CGC
325 GCGC
MflI 161 AGATCC [GA]^GATC[CT]
291 GGATCT
MslI 33 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
51 CATGAAGGTG
MwoI 173 GCGATGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
396 GCATGAATTGC
NlaIII 51 CATG CATG^
397 CATG
NlaIV 311 GGGCCC GG[ACGT]^[ACGT]CC
312 GGCCCC
SfaNI 219 GATGC GCATC(5/9)
256 GATGC
StyI 272 CCAAGG C^C[AT][AT]GG
359 CCAAGG
TseI 222 GCTGC G^C[AT]GC
437 GCTGC
XhoII 161 AGATCC [GA]^GATC[CT]
291 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AluI AlwI BsaJI BseRI
BssKI BstNI Cac8I Cfr13I DpnII
EcoRII Fnu4HI HaeIII ItaI MboI
MboII MnlI MvaI NdeII Sau3AI
Sau96I ScrFI