Restriction map for
pFN21AE1471
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsgI BsiWI
BsmI BsoBI BspDI BspHI BspLU11I
BsrDI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KpnI KspI MamI
MfeI MflI MluI MseI MslI
MunI NciI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfaNI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 494 TCCGGA T^CCGGA
AflIII 272 ACGTGT A^C[GA][CT]GT
AlwI 471 GGATC GGATC(4/5)
AlwNI 502 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 139 GGGCCC GGGCC^C
AscI 177 GGCGCGCC GG^CGCGCC
AspHI 46 GAGCTC G[AT]GC[AT]^C
BalI 128 TGGCCA TGG^CCA
BbsI 576 GAAGAC GAAGAC(2/6)
BfaI 13 CTAG C^TAG
BpmI 43 CTGGAG CTGGAG(16/14)
BpuAI 576 GAAGAC GAAGAC(2/6)
BsaWI 494 TCCGGA [AT]^CCGG[AT]
BseAI 494 TCCGGA T^CCGGA
BsiHKAI 46 GAGCTC G[AT]GC[AT]^C
BsmAI 262 GAGAC GTCTC(1/5)
BsmBI 262 GAGACG CGTCTC(1/5)
Bsp1407I 440 TGTACA T^GTACA
BspEI 494 TCCGGA T^CCGGA
BspMI 501 GCAGGT ACCTGC(4/8)
BsrBI 315 GAGCGG CCGCTC(-3/-3)
BsrGI 440 TGTACA T^GTACA
BssHII 178 GCGCGC G^CGCGC
BssKI 507 CCTGG ^CC[ACGT]GG
BstNI 507 CCTGG CC^[AT]GG
BstXI 551 CCACCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 7 CGGCCG C^GGCCG
EclXI 7 CGGCCG C^GGCCG
Eco52I 7 CGGCCG C^GGCCG
EcoNI 329 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 507 CCTGG ^CC[AT]GG
MaeI 13 CTAG C^TAG
MluNI 128 TGGCCA TGG^CCA
MroI 494 TCCGGA T^CCGGA
MscI 128 TGGCCA TGG^CCA
MspA1I 663 CAGCTG C[AC]G^C[GT]G
MvaI 507 CCTGG CC^[AT]GG
NaeI 588 GCCGGC GCC^GGC
NgoMIV 588 GCCGGC G^CCGGC
PflMI 236 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 503 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 58 CTGCAG CTGCA^G
PvuII 663 CAGCTG CAG^CTG
SacI 46 GAGCTC GAGCT^C
SapI 64 GAAGAGC GCTCTTC(1/4)
ScaI 566 AGTACT AGT^ACT
ScrFI 507 CCTGG CC^[ACGT]GG
SfcI 58 CTGCAG C^T[GA][CT]AG
SspBI 440 TGTACA T^GTACA
TfiI 80 GATTC G^A[AT]TC
TspRI 92 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 236 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbeI 585 GGCGCC GGCGC^C
591 GGCGCC
BsiEI 7 CGGCCG CG[GA][CT]^CG
71 CGGTCG
BsrI 93 ACTGG ACTGG(1/-1)
119 ACTGG
DraII 139 GGGCCCC [GA]G^G[ACGT]CC[CT]
503 AGGTCCT
EarI 613 CTCTTC CTCTTC(1/4)
64 GAAGAG
EcoO109I 139 GGGCCCC [GA]G^G[ACGT]CC[CT]
503 AGGTCCT
EcoT14I 515 CCAAGG C^C[AT][AT]GG
581 CCAAGG
HgaI 427 GACGC GACGC(5/10)
523 GACGC
KasI 585 GGCGCC G^GCGCC
591 GGCGCC
Ksp632I 613 CTCTTC CTCTTC(1/4)
64 GAAGAG
MaeIII 88 GTGAC ^GT[ACGT]AC
533 GTGAC
NarI 585 GGCGCC GG^CGCC
591 GGCGCC
PleI 688 GACTC GAGTC(4/5)
703 GAGTC
SfoI 585 GGCGCC GGC^GCC
591 GGCGCC
SgrAI 296 CGCCGGTG C[GA]^CCGG[CT]G
587 CGCCGGCG
SmlI 49 CTCAAG C^T[CT][GA]AG
184 CTCAAG
StyI 515 CCAAGG C^C[AT][AT]GG
581 CCAAGG
TaqI 74 TCGA T^CGA
197 TCGA
Tsp45I 88 GTGAC ^GT[GC]AC
533 GTGAC
TthHB8I 74 TCGA T^CGA
197 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BanII BbvI BmyI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrFI BstF5I BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EaeI FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeII
MboI MboII MnlI MspI MvnI
MwoI NdeII NlaIV RsaI Sau3AI
Sau96I TseI