Restriction map for
pFN21AE3944
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcyI AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BcnI BfaI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DdeI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI Fnu4HI FseI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI ItaI
KasI KpnI KspI MaeI MaeIII
MflI MluI MluNI MroI MscI
MspA1I MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuII RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI TseI Tsp45I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AclI 112 AACGTT AA^CGTT
AcsI 343 GAATTC [GA]^AATT[CT]
AflII 667 CTTAAG C^TTAAG
ApoI 343 GAATTC [GA]^AATT[CT]
BbsI 634 GAAGAC GAAGAC(2/6)
BclI 72 TGATCA T^GATCA
BfrI 667 CTTAAG C^TTAAG
BpuAI 634 GAAGAC GAAGAC(2/6)
BsaBI 67 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 247 GGTCTC GGTCTC(1/5)
BsaJI 656 CCTTGG C^C[ACGT][ACGT]GG
BsiEI 447 CGATCG CG[GA][CT]^CG
BsiYI 527 CCAAAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 527 CCAAAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 360 GGGAC GGGAC(10/14)
BspHI 683 TCATGA T^CATGA
BsrI 363 ACTGG ACTGG(1/-1)
Cac8I 533 GCAGGC GC[ACGT]^[ACGT]GC
EcoRI 343 GAATTC G^AATTC
EcoT14I 656 CCTTGG C^C[AT][AT]GG
FbaI 72 TGATCA T^GATCA
HinfI 467 GAATC G^A[ACGT]TC
MaeII 113 ACGT A^CGT
MamI 67 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 743 CAATTG C^AATTG
MunI 743 CAATTG C^AATTG
MwoI 331 GCACAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 261 GCATGT [GA]CATG^[CT]
Psp1406I 112 AACGTT AA^CGTT
PvuI 447 CGATCG CGAT^CG
RcaI 683 TCATGA T^CATGA
StyI 656 CCTTGG C^C[AT][AT]GG
SwaI 585 ATTTAAAT ATTT^AAAT
TfiI 467 GAATC G^A[AT]TC
XcmI 527 CCAAAAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AluI 353 AGCT AG^CT
401 AGCT
Asp700I 304 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
343 GAATTCTTTC
BseRI 250 CTCCTC GAGGAG(10/8)
553 GAGGAG
DpnII 73 GATC ^GATC
448 GATC
DraI 586 TTTAAA TTT^AAA
693 TTTAAA
EarI 253 CTCTTC CTCTTC(1/4)
20 GAAGAG
Ksp632I 253 CTCTTC CTCTTC(1/4)
20 GAAGAG
MboI 73 GATC ^GATC
448 GATC
MslI 96 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
751 CACAGCGATG
NdeII 73 GATC ^GATC
448 GATC
NlaIII 262 CATG CATG^
684 CATG
Sau3AI 73 GATC ^GATC
448 GATC
SfaNI 214 GATGC GCATC(5/9)
757 GATGC
SmlI 355 CTCAAG C^T[CT][GA]AG
667 CTTAAG
TaqI 312 TCGA T^CGA
618 TCGA
Tsp509I 344 AATT ^AATT
744 AATT
TspRI 98 CACTG CA[GC]TG[ACGT][ACGT]^
149 CACTG
TthHB8I 312 TCGA T^CGA
618 TCGA
XmnI 304 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
343 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AfaI BsmAI BssKI BstF5I BstNI
EcoRII FokI MboII MnlI MseI
MvaI RsaI ScrFI Tru9I