Restriction map for
pFN21AE6328
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccII AccIII AciI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 138 GTCTAC GT^[AC][GT]AC
AclI 936 AACGTT AA^CGTT
AcsI 542 AAATTC [GA]^AATT[CT]
AfaI 554 GTAC GT^AC
AlwNI 1043 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 542 AAATTC [GA]^AATT[CT]
AseI 558 ATTAAT AT^TAAT
AsnI 558 ATTAAT AT^TAAT
AvaI 442 CTCGAG C^[CT]CG[GA]G
BanI 943 GGTGCC G^G[CT][GA]CC
BciVI 798 GTATCC GTATCC(6/5)
BmyI 994 GTGCCC G[AGT]GC[ACT]^C
BsaI 296 GGTCTC GGTCTC(1/5)
BsgI 592 CTGCAC GTGCAG(16/14)
BsiYI 233 CCAATCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 233 CCAATCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 751 GGGAC GGGAC(10/14)
BsoBI 442 CTCGAG C^[CT]CG[GA]G
Bsp1286I 994 GTGCCC G[AGT]GC[ACT]^C
BspMI 590 ACCTGC ACCTGC(4/8)
BsrI 216 CCAGT ACTGG(1/-1)
BstAPI 1042 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 205 GATC ^GATC
DraII 241 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 1213 GAAGAG CTCTTC(1/4)
EcoO109I 241 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 69 ATGCAT ATGCA^T
HincII 219 GTTAAC GT[CT]^[GA]AC
HindII 219 GTTAAC GT[CT]^[GA]AC
HindIII 825 AAGCTT A^AGCTT
HpaI 219 GTTAAC GTT^AAC
Ksp632I 1213 GAAGAG CTCTTC(1/4)
MaeII 937 ACGT A^CGT
MboI 205 GATC ^GATC
MslI 106 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1046 CAGCTG C[AC]G^C[GT]G
NdeII 205 GATC ^GATC
NsiI 69 ATGCAT ATGCA^T
PaeR7I 442 CTCGAG C^TCGAG
PpuMI 241 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 558 ATTAAT AT^TAAT
Psp1406I 936 AACGTT AA^CGTT
PvuII 1046 CAGCTG CAG^CTG
RsaI 554 GTAC GT^AC
Sau3AI 205 GATC ^GATC
SexAI 57 ACCTGGT A^CC[AT]GGT
SfaNI 846 GATGC GCATC(5/9)
SpeI 1147 ACTAGT A^CTAGT
SphI 1154 GCATGC GCATG^C
SspI 166 AATATT AAT^ATT
TfiI 369 GAATC G^A[AT]TC
XhoI 442 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AflIII 308 ACATGT A^C[GA][CT]GT
794 ACATGT
AvaII 242 GGACC G^G[AT]CC
516 GGACC
BsaJI 245 CCCTGG C^C[ACGT][ACGT]GG
292 CCTCGG
BspLU11I 308 ACATGT A^CATGT
794 ACATGT
Cfr13I 242 GGACC G^G[ACGT]CC
516 GGACC
DraI 177 TTTAAA TTT^AAA
780 TTTAAA
MwoI 1042 GCAGCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1097 GCCTCACTAGC
NlaIV 242 GGACCC GG[ACGT]^[ACGT]CC
943 GGTGCC
PleI 753 GACTC GAGTC(4/5)
1236 GACTC
Sau96I 242 GGACC G^G[ACGT]CC
516 GGACC
SmlI 442 CTCGAG C^T[CT][GA]AG
822 CTCAAG
TaqI 443 TCGA T^CGA
921 TCGA
TthHB8I 443 TCGA T^CGA
921 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BfaI BsmAI BssKI
BstF5I BstNI Cac8I DdeI EcoRII
Fnu4HI FokI HinfI ItaI MaeI
MaeIII MboII MnlI MseI MvaI
NlaIII NspI ScrFI Tru9I TseI
Tsp45I Tsp509I TspRI