Restriction map for
pFN21AE1512
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII KasI KpnI
KspI MaeI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 1040 CCGC CCGC(-3/-1)
Asp700I 915 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 601 TGGCCA TGG^CCA
BclI 177 TGATCA T^GATCA
BglII 717 AGATCT A^GATCT
BsaBI 1157 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 964 GGTCTC GGTCTC(1/5)
BseRI 1012 GAGGAG GAGGAG(10/8)
BsgI 146 CTGCAC GTGCAG(16/14)
BsmFI 496 GGGAC GGGAC(10/14)
BspDI 751 ATCGAT AT^CGAT
BspLU11I 832 ACATGT A^CATGT
BsrDI 506 GCAATG GCAATG(2/0)
BstYI 717 AGATCT [GA]^GATC[CT]
ClaI 751 ATCGAT AT^CGAT
DraI 568 TTTAAA TTT^AAA
DraII 689 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 8 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 601 TGGCCA [CT]^GGCC[GA]
EcoNI 984 CCTACACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 689 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 290 ATGCAT ATGCA^T
FbaI 177 TGATCA T^GATCA
MamI 1157 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 717 AGATCT [GA]^GATC[CT]
MluNI 601 TGGCCA TGG^CCA
MscI 601 TGGCCA TGG^CCA
NcoI 902 CCATGG C^CATGG
NdeI 293 CATATG CA^TATG
NsiI 290 ATGCAT ATGCA^T
NspI 832 ACATGT [GA]CATG^[CT]
PpuMI 689 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 831 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1031 CAGCTG CAG^CTG
SapI 318 GCTCTTC GCTCTTC(1/4)
SmlI 883 CTCAAG C^T[CT][GA]AG
SspI 353 AATATT AAT^ATT
StuI 992 AGGCCT AGG^CCT
XhoII 717 AGATCT [GA]^GATC[CT]
XmnI 915 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 628 GTAC GT^AC
679 GTAC
AflIII 832 ACATGT A^C[GA][CT]GT
890 ACACGT
AvaII 120 GGTCC G^G[AT]CC
690 GGTCC
BsiYI 703 CCATACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
984 CCTACACAAGG
BslI 703 CCATACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
984 CCTACACAAGG
BsmAI 829 GAGAC GTCTC(1/5)
965 GTCTC
Bsp1407I 627 TGTACA T^GTACA
678 TGTACA
BsrGI 627 TGTACA T^GTACA
678 TGTACA
BstXI 686 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
902 CCATGGGAATGG
Cfr13I 120 GGTCC G^G[ACGT]CC
690 GGTCC
DsaI 902 CCATGG C^C[GA][CT]GG
960 CCACGG
EcoRI 3 GAATTC G^AATTC
271 GAATTC
EcoT14I 686 CCAAGG C^C[AT][AT]GG
902 CCATGG
HindIII 259 AAGCTT A^AGCTT
766 AAGCTT
HinfI 394 GAATC G^A[ACGT]TC
1157 GATTC
MaeII 334 ACGT A^CGT
892 ACGT
MaeIII 184 GTCAC ^GT[ACGT]AC
1006 GTCAC
MslI 726 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
903 CATGGGAATG
MspA1I 1031 CAGCTG C[AC]G^C[GT]G
1040 CCGCTG
MwoI 1027 GCTGCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1033 GCTGCCACCGC
NlaIV 130 GGAGCC GG[ACGT]^[ACGT]CC
956 GGAGCC
PstI 760 CTGCAG CTGCA^G
1028 CTGCAG
RsaI 628 GTAC GT^AC
679 GTAC
Sau96I 120 GGTCC G^G[ACGT]CC
690 GGTCC
SspBI 627 TGTACA T^GTACA
678 TGTACA
StyI 686 CCAAGG C^C[AT][AT]GG
902 CCATGG
TfiI 394 GAATC G^A[AT]TC
1157 GATTC
Tsp45I 184 GTCAC ^GT[GC]AC
1006 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI ApoI BbvI BpmI
BsaJI BsrI BssKI BstF5I BstNI
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HphI ItaI Ksp632I
MboI MboII MnlI MseI MvaI
NdeII NlaIII Sau3AI ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I