Restriction map for
pFN21AE1113
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII ClaI CpoI DraIII
DrdI DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 402 GTAC GT^AC
AhdI 438 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 253 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 291 GGGCCC GGGCC^C
AspEI 438 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 394 GGTGCC G^G[CT][GA]CC
BanII 291 GGGCCC G[GA]GC[CT]^C
BbvCI 145 CCTCAGC CCTCAGC(-5/-2)
BclI 219 TGATCA T^GATCA
BsaI 436 GAGACC GGTCTC(1/5)
BseRI 265 GAGGAG GAGGAG(10/8)
BsgI 405 CTGCAC GTGCAG(16/14)
BsrFI 86 ACCGGC [GA]^CCGG[CT]
BsrI 190 ACTGG ACTGG(1/-1)
Cfr10I 86 ACCGGC [GA]^CCGG[CT]
DpnII 220 GATC ^GATC
DraI 226 TTTAAA TTT^AAA
EaeI 284 TGGCCG [CT]^GGCC[GA]
Eam1105I 438 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 258 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 219 TGATCA T^GATCA
HaeII 338 AGCGCC [GA]GCGC^[CT]
HgaI 75 GCGTC GACGC(5/10)
HinfI 140 GACTC G^A[ACGT]TC
HphI 217 GGTGA GGTGA(8/7)
MboI 220 GATC ^GATC
MseI 227 TTAA T^TAA
NdeII 220 GATC ^GATC
PleI 140 GACTC GAGTC(4/5)
RsaI 402 GTAC GT^AC
Sau3AI 220 GATC ^GATC
SexAI 439 ACCAGGT A^CC[AT]GGT
SmaI 37 CCCGGG CCC^GGG
SmlI 276 CTTGAG C^T[CT][GA]AG
Tru9I 227 TTAA T^TAA
XmaCI 37 CCCGGG C^CCGGG
XmaI 37 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 135 CGCG CG^CG
329 CGCG
AvaI 37 CCCGGG C^[CT]CG[GA]G
212 CCCGAG
BmyI 291 GGGCCC G[AGT]GC[ACT]^C
395 GTGCCC
BpmI 94 CTGGAG CTGGAG(16/14)
103 CTGGAG
BsmAI 159 GAGAC GTCTC(1/5)
436 GAGAC
BsoBI 37 CCCGGG C^[CT]CG[GA]G
212 CCCGAG
Bsp1286I 291 GGGCCC G[AGT]GC[ACT]^C
395 GTGCCC
BstF5I 368 CATCC GGATG(2/0)
123 GGATG
BstUI 135 CGCG CG^CG
329 CGCG
CfoI 339 GCGC GCG^C
414 GCGC
DdeI 146 CTCAG C^T[ACGT]AG
167 CTCAG
FokI 368 CATCC GGATG(9/13)
123 GGATG
HhaI 339 GCGC GCG^C
414 GCGC
HinP1I 339 GCGC G^CGC
414 GCGC
MboII 9 GAAGA GAAGA(8/7)
204 GAAGA
MvnI 135 CGCG CG^CG
329 CGCG
PvuII 91 CAGCTG CAG^CTG
387 CAGCTG
SfaNI 181 GATGC GCATC(5/9)
80 GCATC
TspRI 189 CACTG CA[GC]TG[ACGT][ACGT]^
408 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BsaJI BsiYI BslI BsmFI BssKI
BstNI Cfr13I DraII EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HpaII
ItaI MnlI MspA1I MspI MvaI
MwoI NciI NlaIV PpuMI Sau96I
ScrFI TseI