Restriction map for
pFN21AE0497
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# NO CUT ENZYMES ( 121 enzymes) #
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Acc65I AclI AcsI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BpmI BpuAI BsaBI
BsaI BsiWI BsmBI BsmI BsoBI
BspDI BspHI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 949 GACGTC GACGT^C
AccI 310 GTCTAC GT^[AC][GT]AC
AcyI 949 GACGTC G[GA]^CG[CT]C
AflIII 566 ACATGT A^C[GA][CT]GT
ApaI 360 GGGCCC GGGCC^C
AvrII 1143 CCTAGG C^CTAGG
BalI 275 TGGCCA TGG^CCA
BamHI 1011 GGATCC G^GATCC
BcgI 1031 CGACTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1143 CCTAGG C^CTAGG
BlpI 1125 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1125 GCTTAGC GC^T[ACGT]AGC
BsaAI 610 TACGTG [CT]AC^GT[GA]
BsaHI 949 GACGTC G[GA]^CG[CT]C
BsmAI 495 GTCTC GTCTC(1/5)
Bsp1407I 569 TGTACA T^GTACA
BspLU11I 566 ACATGT A^CATGT
BsrGI 569 TGTACA T^GTACA
BstAPI 1160 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1125 GCTTAGC GC^T[ACGT]AGC
DsaI 558 CCACGG C^C[GA][CT]GG
EagI 868 CGGCCG C^GGCCG
EclXI 868 CGGCCG C^GGCCG
Eco52I 868 CGGCCG C^GGCCG
EcoT14I 1143 CCTAGG C^C[AT][AT]GG
HgaI 340 GCGTC GACGC(5/10)
Hin1I 949 GACGTC G[GA]^CG[CT]C
HindIII 874 AAGCTT A^AGCTT
MluNI 275 TGGCCA TGG^CCA
MscI 275 TGGCCA TGG^CCA
MspA1I 691 CAGCTG C[AC]G^C[GT]G
PleI 1032 GACTC GAGTC(4/5)
PpuMI 685 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 691 CAGCTG CAG^CTG
SacI 553 GAGCTC GAGCT^C
SexAI 167 ACCTGGT A^CC[AT]GGT
SspBI 569 TGTACA T^GTACA
StyI 1143 CCTAGG C^C[AT][AT]GG
TaqI 623 TCGA T^CGA
TfiI 326 GAATC G^A[AT]TC
TthHB8I 623 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 50 TCCGGA T^CCGGA
287 TCCGGA
Alw44I 709 GTGCAC G^TGCAC
1118 GTGCAC
AocI 105 CCTGAGG CC^T[ACGT]AGG
519 CCTGAGG
ApaLI 709 GTGCAC G^TGCAC
1118 GTGCAC
BsaWI 50 TCCGGA [AT]^CCGG[AT]
287 TCCGGA
BseAI 50 TCCGGA T^CCGGA
287 TCCGGA
BsiEI 761 CGACCG CG[GA][CT]^CG
868 CGGCCG
BspEI 50 TCCGGA T^CCGGA
287 TCCGGA
BsrBI 21 CCGCTC CCGCTC(-3/-3)
43 GAGCGG
BstYI 986 AGATCC [GA]^GATC[CT]
1011 GGATCC
Bsu36I 105 CCTGAGG CC^T[ACGT]AGG
519 CCTGAGG
DpnII 987 GATC ^GATC
1012 GATC
Eco81I 105 CCTGAGG CC^T[ACGT]AGG
519 CCTGAGG
HinfI 326 GAATC G^A[ACGT]TC
1032 GACTC
MboI 987 GATC ^GATC
1012 GATC
MboII 1051 GAAGA GAAGA(8/7)
1188 GAAGA
MflI 986 AGATCC [GA]^GATC[CT]
1011 GGATCC
MroI 50 TCCGGA T^CCGGA
287 TCCGGA
NdeII 987 GATC ^GATC
1012 GATC
NspI 486 GCATGT [GA]CATG^[CT]
566 ACATGT
PstI 181 CTGCAG CTGCA^G
892 CTGCAG
Sau3AI 987 GATC ^GATC
1012 GATC
SfaNI 101 GCATC GCATC(5/9)
452 GCATC
XcmI 516 CCACCTGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1016 CCATTTTCTCACTGG
XhoII 986 AGATCC [GA]^GATC[CT]
1011 GGATCC
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BcnI BfaI BmyI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
EaeI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeI MaeII MaeIII MnlI MslI
MspI MvaI MvnI MwoI NaeI
NciI NgoMIV NlaIII NlaIV RsaI
Sau96I ScrFI SfcI TseI Tsp45I
TspRI