Restriction map for
pFN21AB7594
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII Eam1105I EarI Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MboII
MfeI MluI MluNI MroI MscI
MslI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 567 GTAC GT^AC
AlwNI 814 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 548 AGCGCT AGC^GCT
AscI 199 GGCGCGCC GG^CGCGCC
BfaI 244 CTAG C^TAG
BglII 530 AGATCT A^GATCT
BpmI 351 CTCCAG CTGGAG(16/14)
BsiEI 425 CGGCCG CG[GA][CT]^CG
BsmBI 170 CGTCTC CGTCTC(1/5)
BsmFI 603 GTCCC GGGAC(10/14)
BspMI 624 GCAGGT ACCTGC(4/8)
BssSI 37 CACGAG CACGAG(-5/-1)
BstAPI 813 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 122 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 238 CTGAG C^T[ACGT]AG
DrdI 619 GACGCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 425 CGGCCG C^GGCCG
EclXI 425 CGGCCG C^GGCCG
Eco47III 548 AGCGCT AGC^GCT
Eco52I 425 CGGCCG C^GGCCG
KspI 103 CCGCGG CCGC^GG
MaeI 244 CTAG C^TAG
MaeII 511 ACGT A^CGT
MseI 71 TTAA T^TAA
NotI 424 GCGGCCGC GC^GGCCGC
PleI 760 GACTC GAGTC(4/5)
PvuII 724 CAGCTG CAG^CTG
RsaI 567 GTAC GT^AC
SacII 103 CCGCGG CCGC^GG
SexAI 634 ACCTGGT A^CC[AT]GGT
TfiI 816 GAATC G^A[AT]TC
Tru9I 71 TTAA T^TAA
Tsp509I 370 AATT ^AATT
TspRI 228 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 108 GGGCCC GGGCC^C
136 GGGCCC
AvaII 81 GGACC G^G[AT]CC
653 GGACC
BsaI 348 GGTCTC GGTCTC(1/5)
364 GGTCTC
BsaWI 479 ACCGGA [AT]^CCGG[AT]
647 ACCGGA
BseRI 679 GAGGAG GAGGAG(10/8)
832 GAGGAG
BsgI 787 CTGCAC GTGCAG(16/14)
526 GTGCAG
BsrFI 270 GCCGGC [GA]^CCGG[CT]
710 GCCGGC
BstF5I 765 CATCC GGATG(2/0)
380 GGATG
Cfr10I 270 GCCGGC [GA]^CCGG[CT]
710 GCCGGC
DsaI 103 CCGCGG C^C[GA][CT]GG
344 CCACGG
EaeI 425 CGGCCG [CT]^GGCC[GA]
462 CGGCCA
EcoT14I 357 CCTTGG C^C[AT][AT]GG
845 CCAAGG
FokI 765 CATCC GGATG(9/13)
380 GGATG
HinfI 760 GACTC G^A[ACGT]TC
816 GAATC
HphI 443 TCACC GGTGA(8/7)
860 TCACC
NaeI 270 GCCGGC GCC^GGC
710 GCCGGC
NgoMIV 270 GCCGGC G^CCGGC
710 GCCGGC
NlaIII 330 CATG CATG^
717 CATG
SfaNI 56 GCATC GCATC(5/9)
458 GCATC
StyI 357 CCTTGG C^C[AT][AT]GG
845 CCAAGG
TaqI 59 TCGA T^CGA
209 TCGA
TthHB8I 59 TCGA T^CGA
209 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI BanI BanII BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrBI BssHII BssKI BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI KasI
MaeIII MboI MflI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIV PstI Sau3AI
Sau96I ScrFI SfcI SfoI SmaI
TseI Tsp45I XhoII XmaCI XmaI