Restriction map for
pFN21AE1516
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# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI AcsI AflII AhdI AocI
Aor51HI ApoI AscI AseI AsnI
AspEI AvrII BamHI BciVI BfrI
BlnI BsiWI BspDI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI DraI
Eam1105I Eco47III Eco81I EcoRI EcoRV
EcoT22I FseI HpaI MfeI MluI
MunI NdeI NruI NsiI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 3676 GACGTC GACGT^C
Acc65I 1139 GGTACC G^GTACC
AccIII 4142 TCCGGA T^CCGGA
AgeI 34 ACCGGT A^CCGGT
Alw44I 751 GTGCAC G^TGCAC
ApaLI 751 GTGCAC G^TGCAC
Asp718I 1139 GGTACC G^GTACC
BbeI 1927 GGCGCC GGCGC^C
BcgI 198 CGAGCTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 4142 TCCGGA T^CCGGA
BsmBI 4303 GAGACG CGTCTC(1/5)
BsmI 4176 GAATGC GAATGC(1/-1)
BspEI 4142 TCCGGA T^CCGGA
BsrBI 3190 GAGCGG CCGCTC(-3/-3)
BsrDI 3533 GCAATG GCAATG(2/0)
CpoI 4307 CGGACCG CG^G[AT]CCG
HindIII 288 AAGCTT A^AGCTT
KasI 1927 GGCGCC G^GCGCC
KpnI 1139 GGTACC GGTAC^C
KspI 2952 CCGCGG CCGC^GG
MroI 4142 TCCGGA T^CCGGA
MseI 2675 TTAA T^TAA
NarI 1927 GGCGCC GG^CGCC
NheI 4251 GCTAGC G^CTAGC
NotI 1093 GCGGCCGC GC^GGCCGC
PaeR7I 196 CTCGAG C^TCGAG
PinAI 34 ACCGGT A^CCGGT
RsrII 4307 CGGACCG CG^G[AT]CCG
SacI 2158 GAGCTC GAGCT^C
SacII 2952 CCGCGG CCGC^GG
SalI 2914 GTCGAC G^TCGAC
SfiI 2167 GGCCGCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1927 GGCGCC GGC^GCC
Tru9I 2675 TTAA T^TAA
Tsp509I 1168 AATT ^AATT
XhoI 196 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 460 GTCTAC GT^[AC][GT]AC
2914 GTCGAC
AlwNI 619 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
799 CAGGCTCTG
BclI 3419 TGATCA T^GATCA
3644 TGATCA
BglI 2168 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3347 GCCTCAAAGGC
BglII 1028 AGATCT A^GATCT
2384 AGATCT
BlpI 3726 GCTGAGC GC^T[ACGT]AGC
3966 GCTCAGC
Bpu1102I 3726 GCTGAGC GC^T[ACGT]AGC
3966 GCTCAGC
BsaBI 1002 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3414 GATCTTGATC
BsaWI 34 ACCGGT [AT]^CCGG[AT]
4142 TCCGGA
BspHI 1973 TCATGA T^CATGA
3680 TCATGA
BspLU11I 1601 ACATGT A^CATGT
3842 ACATGT
BssSI 517 CACGAG CACGAG(-5/-1)
526 CTCGTG
BstAPI 618 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
744 GCAGAAGGTGC
BstEII 1122 GGTCACC G^GT[ACGT]ACC
2862 GGTGACC
BstPI 1122 GGTCACC G^GT[ACGT]ACC
2862 GGTGACC
CelII 3726 GCTGAGC GC^T[ACGT]AGC
3966 GCTCAGC
DrdI 997 GACAAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2905 GACGGCCCGGTC
EcoO65I 1122 GGTCACC G^GT[ACGT]ACC
2862 GGTGACC
FbaI 3419 TGATCA T^GATCA
3644 TGATCA
MamI 1002 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3414 GATCTTGATC
NaeI 1282 GCCGGC GCC^GGC
4120 GCCGGC
NgoMIV 1282 GCCGGC G^CCGGC
4120 GCCGGC
RcaI 1973 TCATGA T^CATGA
3680 TCATGA
SexAI 86 ACCTGGT A^CC[AT]GGT
3542 ACCTGGT
SphI 1058 GCATGC GCATG^C
3878 GCATGC
StuI 2624 AGGCCT AGG^CCT
3215 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI Asp700I AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StyI TaqI TfiI TseI
Tsp45I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI