Restriction map for
pFN21AE4536
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
Eam1105I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MaeI MfeI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI TfiI Tsp509I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 463 GTCTAC GT^[AC][GT]AC
AflIII 587 ACGCGT A^C[GA][CT]GT
Aor51HI 1471 AGCGCT AGC^GCT
AspI 778 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 847 CACGTG CAC^GTG
BbvCI 1661 CCTCAGC CCTCAGC(-5/-2)
BglII 252 AGATCT A^GATCT
BsaBI 1167 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 725 GCAATG GCAATG(2/0)
BssSI 256 CTCGTG CACGAG(-5/-1)
BstXI 38 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 445 GACGGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1239 CGGCCG C^GGCCG
EarI 1726 GAAGAG CTCTTC(1/4)
EclXI 1239 CGGCCG C^GGCCG
Eco47III 1471 AGCGCT AGC^GCT
Eco52I 1239 CGGCCG C^GGCCG
EcoRV 935 GATATC GAT^ATC
HincII 1423 GTCAAC GT[CT]^[GA]AC
HindII 1423 GTCAAC GT[CT]^[GA]AC
Ksp632I 1726 GAAGAG CTCTTC(1/4)
MamI 1167 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 587 ACGCGT A^CGCGT
MseI 659 TTAA T^TAA
NcoI 596 CCATGG C^CATGG
NspI 1301 GCATGT [GA]CATG^[CT]
PflFI 778 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 847 CACGTG CAC^GTG
PmlI 847 CACGTG CAC^GTG
SapI 1726 GAAGAGC GCTCTTC(1/4)
ScaI 1499 AGTACT AGT^ACT
SfaNI 1001 GCATC GCATC(5/9)
SfiI 855 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 381 AGGCCT AGG^CCT
Tru9I 659 TTAA T^TAA
Tth111I 778 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AocI 995 CCTCAGG CC^T[ACGT]AGG
1549 CCTCAGG
ApaI 799 GGGCCC GGGCC^C
863 GGGCCC
Asp700I 1497 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1590 GAACTCCTTC
AvaI 513 CTCGGG C^[CT]CG[GA]G
769 CCCGAG
BbsI 1369 GAAGAC GAAGAC(2/6)
754 GTCTTC
BglI 856 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1766 GCCCTGCTGGC
BpuAI 1369 GAAGAC GAAGAC(2/6)
754 GTCTTC
BsaI 637 GAGACC GGTCTC(1/5)
1099 GAGACC
BsaWI 488 TCCGGT [AT]^CCGG[AT]
570 ACCGGA
BsoBI 513 CTCGGG C^[CT]CG[GA]G
769 CCCGAG
BsrFI 901 GCCGGC [GA]^CCGG[CT]
1483 GCCGGC
BstAPI 534 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1695 GCAGCCCCTGC
Bsu36I 995 CCTCAGG CC^T[ACGT]AGG
1549 CCTCAGG
Cfr10I 901 GCCGGC [GA]^CCGG[CT]
1483 GCCGGC
Eco81I 995 CCTCAGG CC^T[ACGT]AGG
1549 CCTCAGG
EcoNI 435 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1701 CCTGCCCAAGG
HaeII 1031 GGCGCT [GA]GCGC^[CT]
1471 AGCGCT
HinfI 808 GACTC G^A[ACGT]TC
991 GACTC
NaeI 901 GCCGGC GCC^GGC
1483 GCCGGC
NgoMIV 901 GCCGGC G^CCGGC
1483 GCCGGC
PleI 808 GACTC GAGTC(4/5)
991 GACTC
SacI 136 GAGCTC GAGCT^C
1459 GAGCTC
SmlI 199 CTCAAG C^T[CT][GA]AG
241 CTTGAG
XmnI 1497 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1590 GAACTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaII BalI BamHI BanI BanII
BbvI BcnI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SspBI StyI TseI Tsp45I TspRI
Van91I XhoII