Restriction map for
pFN21AE5611
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# NO CUT ENZYMES ( 121 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BbeI BbrPI BcgI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI DraI DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI SacII SalI
SbfI ScaI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tru9I Tsp509I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 610 GACGTC GACGT^C
AccI 937 GTCTAC GT^[AC][GT]AC
AfaI 1140 GTAC GT^AC
AflIII 239 ACGTGT A^C[GA][CT]GT
AocI 510 CCTGAGG CC^T[ACGT]AGG
Asp700I 789 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 709 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 351 TGCGCA TGC^GCA
BalI 806 TGGCCA TGG^CCA
BbsI 307 GTCTTC GAAGAC(2/6)
BbvCI 160 CCTCAGC CCTCAGC(-5/-2)
BciVI 522 GTATCC GTATCC(6/5)
BfaI 568 CTAG C^TAG
BpuAI 307 GTCTTC GAAGAC(2/6)
BsgI 1053 CTGCAC GTGCAG(16/14)
BsiEI 284 CGGCCG CG[GA][CT]^CG
BsmI 789 GAATGC GAATGC(1/-1)
BstYI 1359 AGATCC [GA]^GATC[CT]
Bsu36I 510 CCTGAGG CC^T[ACGT]AGG
CpoI 713 CGGTCCG CG^G[AT]CCG
DraIII 529 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1370 CCGTGG C^C[GA][CT]GG
EagI 284 CGGCCG C^GGCCG
EarI 772 CTCTTC CTCTTC(1/4)
EclXI 284 CGGCCG C^GGCCG
Eco52I 284 CGGCCG C^GGCCG
Eco81I 510 CCTGAGG CC^T[ACGT]AGG
EcoNI 506 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 351 TGCGCA TGC^GCA
Ksp632I 772 CTCTTC CTCTTC(1/4)
MaeI 568 CTAG C^TAG
MflI 1359 AGATCC [GA]^GATC[CT]
MluNI 806 TGGCCA TGG^CCA
MscI 806 TGGCCA TGG^CCA
MslI 988 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 709 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 990 CAGCTG CAG^CTG
RsaI 1140 GTAC GT^AC
RsrII 713 CGGTCCG CG^G[AT]CCG
SacI 1126 GAGCTC GAGCT^C
SapI 771 GCTCTTC GCTCTTC(1/4)
SexAI 530 ACCTGGT A^CC[AT]GGT
SfcI 694 CTACAG C^T[GA][CT]AG
SfiI 66 GGCCCGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 1272 GATTC G^A[AT]TC
Tth111I 709 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1359 AGATCC [GA]^GATC[CT]
XmnI 789 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 610 GACGTC G[GA]^CG[CT]C
1027 GACGCC
AlwI 1360 GATCC GGATC(4/5)
905 GGATC
BanI 730 GGCACC G^G[CT][GA]CC
1015 GGCACC
BglI 67 GCCCGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1173 GCCCGACTGGC
BpmI 55 CTCCAG CTGGAG(16/14)
1390 CTGGAG
BsaHI 610 GACGTC G[GA]^CG[CT]C
1027 GACGCC
BspMI 733 ACCTGC ACCTGC(4/8)
1109 ACCTGC
BsrBI 8 CCGCTC CCGCTC(-3/-3)
289 GAGCGG
BsrFI 977 ACCGGC [GA]^CCGG[CT]
1018 ACCGGC
BssHII 428 GCGCGC G^CGCGC
557 GCGCGC
Cfr10I 977 ACCGGC [GA]^CCGG[CT]
1018 ACCGGC
Hin1I 610 GACGTC G[GA]^CG[CT]C
1027 GACGCC
MaeII 239 ACGT A^CGT
611 ACGT
NlaIII 777 CATG CATG^
810 CATG
PpuMI 758 AGGACCT [GA]G^G[AT]CC[CT]
843 GGGTCCC
SfaNI 457 GATGC GCATC(5/9)
495 GATGC
StuI 579 AGGCCT AGG^CCT
1049 AGGCCT
TaqI 134 TCGA T^CGA
1334 TCGA
TthHB8I 134 TCGA T^CGA
1334 TCGA
XcmI 830 CCACTGACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1238 CCAACGGGAACCTGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AluI AlwNI Aor51HI
ApaI AspHI AvaI AvaII BanII
BbvI BcnI BmyI BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI Eco47III EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PflMI PleI Sau3AI Sau96I ScrFI
TseI Tsp45I TspRI Van91I