Restriction map for
pFN21AE3607
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BlnI BsaAI BsaBI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI HaeII HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AcsI 595 GAATTC [GA]^AATT[CT]
AcyI 520 GACGCC G[GA]^CG[CT]C
AfaI 555 GTAC GT^AC
AflIII 742 ACATGT A^C[GA][CT]GT
AlwI 27 GGATC GGATC(4/5)
AocI 57 CCTGAGG CC^T[ACGT]AGG
ApoI 595 GAATTC [GA]^AATT[CT]
AseI 205 ATTAAT AT^TAAT
AsnI 205 ATTAAT AT^TAAT
AvaII 177 GGACC G^G[AT]CC
BciVI 318 GGATAC GTATCC(6/5)
BglII 152 AGATCT A^GATCT
BlpI 643 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 643 GCTGAGC GC^T[ACGT]AGC
BsaHI 520 GACGCC G[GA]^CG[CT]C
BsaWI 128 ACCGGA [AT]^CCGG[AT]
BseRI 368 GAGGAG GAGGAG(10/8)
BsmAI 565 GTCTC GTCTC(1/5)
BsmFI 176 GGGAC GGGAC(10/14)
BsmI 293 GCATTC GAATGC(1/-1)
BspLU11I 742 ACATGT A^CATGT
BspMI 109 ACCTGC ACCTGC(4/8)
BsrDI 348 CATTGC GCAATG(2/0)
BstF5I 678 GGATG GGATG(2/0)
BstYI 152 AGATCT [GA]^GATC[CT]
Bsu36I 57 CCTGAGG CC^T[ACGT]AGG
CelII 643 GCTGAGC GC^T[ACGT]AGC
DraII 176 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 140 CCGTGG C^C[GA][CT]GG
EaeI 440 TGGCCG [CT]^GGCC[GA]
EarI 481 CTCTTC CTCTTC(1/4)
Eco81I 57 CCTGAGG CC^T[ACGT]AGG
EcoNI 57 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 176 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 595 GAATTC G^AATTC
EcoT14I 158 CCAAGG C^C[AT][AT]GG
EcoT22I 345 ATGCAT ATGCA^T
FauI 101 CCCGC CCCGC(4/6)
FokI 678 GGATG GGATG(9/13)
HapII 129 CCGG C^CGG
Hin1I 520 GACGCC G[GA]^CG[CT]C
HpaII 129 CCGG C^CGG
Ksp632I 481 CTCTTC CTCTTC(1/4)
MaeIII 106 GTCAC ^GT[ACGT]AC
MflI 152 AGATCT [GA]^GATC[CT]
MspA1I 323 CCGCTG C[AC]G^C[GT]G
MspI 129 CCGG C^CGG
NlaIII 743 CATG CATG^
NsiI 345 ATGCAT ATGCA^T
NspI 742 ACATGT [GA]CATG^[CT]
PflMI 428 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 176 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 205 ATTAAT AT^TAAT
RsaI 555 GTAC GT^AC
SacI 371 GAGCTC GAGCT^C
ScaI 554 AGTACT AGT^ACT
SfcI 363 CTATAG C^T[GA][CT]AG
SmlI 374 CTCAAG C^T[CT][GA]AG
StyI 158 CCAAGG C^C[AT][AT]GG
Tsp45I 106 GTCAC ^GT[GC]AC
Van91I 428 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 152 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 31 CGCG CG^CG
103 CGCG
AlwNI 52 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
433 CAGTGGCTG
AspHI 124 GAGCAC G[AT]GC[AT]^C
371 GAGCTC
BanI 12 GGCACC G^G[CT][GA]CC
136 GGCACC
BanII 371 GAGCTC G[GA]GC[CT]^C
662 GGGCTC
BbsI 192 GAAGAC GAAGAC(2/6)
391 GTCTTC
BpmI 526 CTCCAG CTGGAG(16/14)
649 CTGGAG
BpuAI 192 GAAGAC GAAGAC(2/6)
391 GTCTTC
BsiHKAI 124 GAGCAC G[AT]GC[AT]^C
371 GAGCTC
BstUI 31 CGCG CG^CG
103 CGCG
BstXI 251 CCATTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
632 CCAATGTGCTGG
CfoI 113 GCGC GCG^C
505 GCGC
Cfr13I 177 GGACC G^G[ACGT]CC
534 GGCCC
DpnII 28 GATC ^GATC
153 GATC
HhaI 113 GCGC GCG^C
505 GCGC
HinP1I 113 GCGC G^CGC
505 GCGC
HphI 580 GGTGA GGTGA(8/7)
107 TCACC
MboI 28 GATC ^GATC
153 GATC
MseI 149 TTAA T^TAA
206 TTAA
MslI 165 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
252 CATTGGAGTG
MvnI 31 CGCG CG^CG
103 CGCG
NdeII 28 GATC ^GATC
153 GATC
Sau3AI 28 GATC ^GATC
153 GATC
Sau96I 177 GGACC G^G[ACGT]CC
534 GGCCC
TaqI 266 TCGA T^CGA
666 TCGA
Tru9I 149 TTAA T^TAA
206 TTAA
Tsp509I 238 AATT ^AATT
596 AATT
TthHB8I 266 TCGA T^CGA
666 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I DdeI EcoRII Fnu4HI
HaeIII HgaI ItaI MboII MnlI
MvaI MwoI NlaIV ScrFI SfaNI
TseI TspRI XcmI