Restriction map for
pFN21AE4537
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BbvCI BcgI
BclI BglII BlnI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HindIII HpaI KasI KpnI MamI
MluI MroI NarI NdeI NheI
NotI NsiI NspI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 241 GACGCC G[GA]^CG[CT]C
AflII 1662 CTTAAG C^TTAAG
Asp700I 381 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 616 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1272 GGATCC G^GATCC
BanI 258 GGCACC G^G[CT][GA]CC
BanII 88 GAGCCC G[GA]GC[CT]^C
BciVI 1454 GGATAC GTATCC(6/5)
BfrI 1662 CTTAAG C^TTAAG
BlpI 1681 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1681 GCTCAGC GC^T[ACGT]AGC
BsaAI 565 CACGTA [CT]AC^GT[GA]
BsaHI 241 GACGCC G[GA]^CG[CT]C
BsmFI 514 GGGAC GGGAC(10/14)
BspMI 1308 ACCTGC ACCTGC(4/8)
BsrDI 1325 GCAATG GCAATG(2/0)
CelII 1681 GCTCAGC GC^T[ACGT]AGC
DraI 1600 TTTAAA TTT^AAA
DraII 247 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 821 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 883 GACACAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1615 GAAGAG CTCTTC(1/4)
EcoO109I 247 GGGGCCT [GA]G^G[ACGT]CC[CT]
Hin1I 241 GACGCC G[GA]^CG[CT]C
HincII 1545 GTCAAC GT[CT]^[GA]AC
HindII 1545 GTCAAC GT[CT]^[GA]AC
Ksp632I 1615 GAAGAG CTCTTC(1/4)
MfeI 1484 CAATTG C^AATTG
MunI 1484 CAATTG C^AATTG
NcoI 1276 CCATGG C^CATGG
NruI 225 TCGCGA TCG^CGA
PaeR7I 1370 CTCGAG C^TCGAG
PflFI 616 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1358 CAGCTG CAG^CTG
SmaI 347 CCCGGG CCC^GGG
SpeI 1102 ACTAGT A^CTAGT
Tth111I 616 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1758 CCAGAAGAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1370 CTCGAG C^TCGAG
XmaCI 347 CCCGGG C^CCGGG
XmaI 347 CCCGGG C^CCGGG
XmnI 381 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 12 GTAC GT^AC
57 GTAC
BbsI 31 GTCTTC GAAGAC(2/6)
497 GTCTTC
BpuAI 31 GTCTTC GAAGAC(2/6)
497 GTCTTC
BseRI 997 GAGGAG GAGGAG(10/8)
1267 GAGGAG
BstAPI 337 GCACAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1077 GCAGCGTATGC
BstYI 1272 GGATCC [GA]^GATC[CT]
1407 AGATCC
CfoI 416 GCGC GCG^C
1433 GCGC
DpnII 1273 GATC ^GATC
1408 GATC
EagI 154 CGGCCG C^GGCCG
164 CGGCCG
EclXI 154 CGGCCG C^GGCCG
164 CGGCCG
Eco52I 154 CGGCCG C^GGCCG
164 CGGCCG
EcoRI 593 GAATTC G^AATTC
1290 GAATTC
HhaI 416 GCGC GCG^C
1433 GCGC
HinP1I 416 GCGC G^CGC
1433 GCGC
KspI 121 CCGCGG CCGC^GG
151 CCGCGG
MaeII 68 ACGT A^CGT
566 ACGT
MboI 1273 GATC ^GATC
1408 GATC
MflI 1272 GGATCC [GA]^GATC[CT]
1407 AGATCC
NdeII 1273 GATC ^GATC
1408 GATC
RsaI 12 GTAC GT^AC
57 GTAC
SacII 121 CCGCGG CCGC^GG
151 CCGCGG
Sau3AI 1273 GATC ^GATC
1408 GATC
SfaNI 970 GATGC GCATC(5/9)
1121 GCATC
SmlI 1370 CTCGAG C^T[CT][GA]AG
1662 CTTAAG
XhoII 1272 GGATCC [GA]^GATC[CT]
1407 AGATCC
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AspHI AvaI AvaII BalI
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BsiEI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr10I Cfr13I DdeI DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MaeI MaeIII
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIII
NlaIV PleI Sau96I ScrFI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I