Restriction map for
pFN21AE1422
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwNI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BsrI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 558 TCCGGA T^CCGGA
Alw44I 1261 GTGCAC G^TGCAC
AocI 848 CCTCAGG CC^T[ACGT]AGG
ApaLI 1261 GTGCAC G^TGCAC
AspI 936 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 406 TGCGCA TGC^GCA
AvrII 117 CCTAGG C^CTAGG
BanI 532 GGTGCC G^G[CT][GA]CC
BcnI 141 CCGGG CC^[GC]GG
BlnI 117 CCTAGG C^CTAGG
BsaI 934 GAGACC GGTCTC(1/5)
BseAI 558 TCCGGA T^CCGGA
BsmAI 934 GAGAC GTCTC(1/5)
BspEI 558 TCCGGA T^CCGGA
BspMI 950 ACCTGC ACCTGC(4/8)
BssHII 375 GCGCGC G^CGCGC
Bsu36I 848 CCTCAGG CC^T[ACGT]AGG
DraIII 592 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 848 CCTCAGG CC^T[ACGT]AGG
EcoNI 1158 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 117 CCTAGG C^C[AT][AT]GG
FspI 406 TGCGCA TGC^GCA
MaeII 826 ACGT A^CGT
MroI 558 TCCGGA T^CCGGA
MseI 812 TTAA T^TAA
NciI 141 CCGGG CC^[GC]GG
PaeR7I 69 CTCGAG C^TCGAG
PflFI 936 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1232 GGGACCC [GA]G^G[AT]CC[CT]
SfcI 791 CTGTAG C^T[GA][CT]AG
StuI 311 AGGCCT AGG^CCT
StyI 117 CCTAGG C^C[AT][AT]GG
Tru9I 812 TTAA T^TAA
Tsp45I 166 GTCAC ^GT[GC]AC
Tsp509I 1050 AATT ^AATT
Tth111I 936 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1028 CCAGCGCTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 69 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 306 GTAC GT^AC
1177 GTAC
AlwI 495 GATCC GGATC(4/5)
698 GATCC
Aor51HI 1030 AGCGCT AGC^GCT
1220 AGCGCT
AvaI 69 CTCGAG C^[CT]CG[GA]G
1095 CTCGGG
AvaII 600 GGACC G^G[AT]CC
1233 GGACC
BanII 72 GAGCCC G[GA]GC[CT]^C
261 GGGCTC
BbsI 646 GAAGAC GAAGAC(2/6)
946 GAAGAC
BpuAI 646 GAAGAC GAAGAC(2/6)
946 GAAGAC
BsaWI 529 TCCGGT [AT]^CCGG[AT]
558 TCCGGA
BsoBI 69 CTCGAG C^[CT]CG[GA]G
1095 CTCGGG
BstXI 33 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1028 CCAGCGCTCTGG
BstYI 494 AGATCC [GA]^GATC[CT]
697 AGATCC
DraII 638 AGGCCCT [GA]G^G[ACGT]CC[CT]
1232 GGGACCC
DsaI 536 CCGTGG C^C[GA][CT]GG
596 CCGTGG
EarI 985 GAAGAG CTCTTC(1/4)
1138 GAAGAG
Eco47III 1030 AGCGCT AGC^GCT
1220 AGCGCT
EcoO109I 638 AGGCCCT [GA]G^G[ACGT]CC[CT]
1232 GGGACCC
FauI 353 CCCGC CCCGC(4/6)
1246 CCCGC
HinfI 991 GAGTC G^A[ACGT]TC
1147 GAGTC
Ksp632I 985 GAAGAG CTCTTC(1/4)
1138 GAAGAG
MaeIII 166 GTCAC ^GT[ACGT]AC
1091 GTAAC
MflI 494 AGATCC [GA]^GATC[CT]
697 AGATCC
NspI 50 ACATGC [GA]CATG^[CT]
200 ACATGC
PleI 991 GAGTC GAGTC(4/5)
1147 GAGTC
RsaI 306 GTAC GT^AC
1177 GTAC
SmlI 69 CTCGAG C^T[CT][GA]AG
724 CTCAAG
XhoII 494 AGATCC [GA]^GATC[CT]
697 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI AspHI BbvI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeI MboI MboII
MnlI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I