Restriction map for
pFN21AE6312
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstYI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I Tth111I XbaI XcmI
XhoII
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwNI 118 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 228 CCTGAGG CC^T[ACGT]AGG
ApaI 867 GGGCCC GGGCC^C
Asp700I 837 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 126 GGACC G^G[AT]CC
BanII 867 GGGCCC G[GA]GC[CT]^C
BciVI 209 GTATCC GTATCC(6/5)
BclI 680 TGATCA T^GATCA
BmyI 867 GGGCCC G[AGT]GC[ACT]^C
BsaBI 504 GATTCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 140 ACCGGA [AT]^CCGG[AT]
BsmAI 926 GTCTC GTCTC(1/5)
BsmBI 925 CGTCTC CGTCTC(1/5)
Bsp1286I 867 GGGCCC G[AGT]GC[ACT]^C
BspMI 385 GCAGGT ACCTGC(4/8)
BsrBI 153 GAGCGG CCGCTC(-3/-3)
BsrDI 723 CATTGC GCAATG(2/0)
BstBI 506 TTCGAA TT^CGAA
Bsu36I 228 CCTGAGG CC^T[ACGT]AGG
DpnII 681 GATC ^GATC
DraIII 534 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 228 CCTGAGG CC^T[ACGT]AGG
EcoNI 831 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 680 TGATCA T^GATCA
MaeII 791 ACGT A^CGT
MaeIII 694 GTCAC ^GT[ACGT]AC
MamI 504 GATTCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 681 GATC ^GATC
MseI 494 TTAA T^TAA
NcoI 560 CCATGG C^CATGG
NdeII 681 GATC ^GATC
NspI 746 ACATGC [GA]CATG^[CT]
NspV 506 TTCGAA TT^CGAA
PaeR7I 173 CTCGAG C^TCGAG
PflMI 117 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 461 CAGCTG CAG^CTG
Sau3AI 681 GATC ^GATC
SexAI 128 ACCTGGT A^CC[AT]GGT
SfcI 104 CTACAG C^T[GA][CT]AG
SfuI 506 TTCGAA TT^CGAA
SmaI 515 CCCGGG CCC^GGG
SmlI 173 CTCGAG C^T[CT][GA]AG
Tru9I 494 TTAA T^TAA
Tsp45I 694 GTCAC ^GT[GC]AC
Van91I 117 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 173 CTCGAG C^TCGAG
XmaCI 515 CCCGGG C^CCGGG
XmaI 515 CCCGGG C^CCGGG
XmnI 837 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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BanI 388 GGTGCC G^G[CT][GA]CC
397 GGTGCC
BglI 227 GCCTGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
418 GCCGAAGTGGC
BsrFI 25 GCCGGC [GA]^CCGG[CT]
452 GCCGGC
BstXI 468 CCAGCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
710 CCATGCCTGTGG
CfoI 321 GCGC GCG^C
471 GCGC
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
452 GCCGGC
DraII 83 GGGGCCT [GA]G^G[ACGT]CC[CT]
867 GGGCCCC
DsaI 474 CCGTGG C^C[GA][CT]GG
560 CCATGG
EaeI 89 TGGCCG [CT]^GGCC[GA]
450 TGGCCG
EarI 928 CTCTTC CTCTTC(1/4)
520 GAAGAG
EcoO109I 83 GGGGCCT [GA]G^G[ACGT]CC[CT]
867 GGGCCCC
EcoT14I 294 CCTTGG C^C[AT][AT]GG
560 CCATGG
HaeII 320 AGCGCC [GA]GCGC^[CT]
470 AGCGCC
HgaI 267 GCGTC GACGC(5/10)
924 GCGTC
HhaI 321 GCGC GCG^C
471 GCGC
HinP1I 321 GCGC G^CGC
471 GCGC
Ksp632I 928 CTCTTC CTCTTC(1/4)
520 GAAGAG
MspA1I 461 CAGCTG C[AC]G^C[GT]G
684 CAGCGG
NaeI 25 GCCGGC GCC^GGC
452 GCCGGC
NgoMIV 25 GCCGGC G^CCGGC
452 GCCGGC
PleI 171 GACTC GAGTC(4/5)
631 GACTC
StuI 225 AGGCCT AGG^CCT
914 AGGCCT
StyI 294 CCTTGG C^C[AT][AT]GG
560 CCATGG
TaqI 174 TCGA T^CGA
507 TCGA
TthHB8I 174 TCGA T^CGA
507 TCGA
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AvaI BbvI
BcnI BsaJI BsiYI BslI BsoBI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboII MnlI MslI
MspI MvaI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TfiI TseI TspRI