Restriction map for
pFN21AE1466
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvrII
BalI BamHI BanII BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiHKAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
DraI DraII Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MluNI MroI MscI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 299 GGCGCC G[GA]^CG[CT]C
AflIII 1583 ACGCGT A^C[GA][CT]GT
AgeI 1272 ACCGGT A^CCGGT
AvaI 1254 CCCGAG C^[CT]CG[GA]G
AviII 1566 TGCGCA TGC^GCA
BbeI 299 GGCGCC GGCGC^C
BcnI 1657 CCGGG CC^[GC]GG
BglII 1356 AGATCT A^GATCT
BsaHI 299 GGCGCC G[GA]^CG[CT]C
BsgI 220 CTGCAC GTGCAG(16/14)
BsiEI 1820 CGGCCG CG[GA][CT]^CG
BsmBI 1763 CGTCTC CGTCTC(1/5)
BsmI 316 GCATTC GAATGC(1/-1)
BsoBI 1254 CCCGAG C^[CT]CG[GA]G
BsrBI 1031 CCGCTC CCGCTC(-3/-3)
BsrDI 1373 GCAATG GCAATG(2/0)
BstAPI 222 GCACGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1356 AGATCT [GA]^GATC[CT]
CpoI 1364 CGGACCG CG^G[AT]CCG
DraIII 223 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1271 GACCGGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1820 CGGCCG C^GGCCG
EclXI 1820 CGGCCG C^GGCCG
Eco52I 1820 CGGCCG C^GGCCG
EcoRV 1513 GATATC GAT^ATC
FspI 1566 TGCGCA TGC^GCA
HaeII 299 GGCGCC [GA]GCGC^[CT]
Hin1I 299 GGCGCC G[GA]^CG[CT]C
KasI 299 GGCGCC G^GCGCC
MaeII 908 ACGT A^CGT
MfeI 689 CAATTG C^AATTG
MflI 1356 AGATCT [GA]^GATC[CT]
MluI 1583 ACGCGT A^CGCGT
MunI 689 CAATTG C^AATTG
NaeI 1324 GCCGGC GCC^GGC
NarI 299 GGCGCC GG^CGCC
NciI 1657 CCGGG CC^[GC]GG
NcoI 1664 CCATGG C^CATGG
NdeI 830 CATATG CA^TATG
NgoMIV 1324 GCCGGC G^CCGGC
PinAI 1272 ACCGGT A^CCGGT
PstI 605 CTGCAG CTGCA^G
PvuII 1739 CAGCTG CAG^CTG
RsrII 1364 CGGACCG CG^G[AT]CCG
SfoI 299 GGCGCC GGC^GCC
SgrAI 301 CGCCGGTG C[GA]^CCGG[CT]G
SspI 822 AATATT AAT^ATT
XcmI 587 CCAACGGGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1356 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 1584 CGCG CG^CG
1849 CGCG
Asp700I 409 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1108 GAAATGCTTC
BbsI 426 GAAGAC GAAGAC(2/6)
252 GTCTTC
BbvCI 1700 CCTCAGC CCTCAGC(-5/-2)
1854 GCTGAGG
BmyI 229 GTGCCC G[AGT]GC[ACT]^C
1708 GTGCCC
BpmI 206 CTCCAG CTGGAG(16/14)
761 CTCCAG
BpuAI 426 GAAGAC GAAGAC(2/6)
252 GTCTTC
BsaI 1269 GAGACC GGTCTC(1/5)
1870 GAGACC
BsaWI 981 ACCGGA [AT]^CCGG[AT]
1272 ACCGGT
Bsp1286I 229 GTGCCC G[AGT]GC[ACT]^C
1708 GTGCCC
BstUI 1584 CGCG CG^CG
1849 CGCG
EarI 1537 GAAGAG CTCTTC(1/4)
1927 GAAGAG
EcoT14I 682 CCAAGG C^C[AT][AT]GG
1664 CCATGG
HgaI 1582 GACGC GACGC(5/10)
1585 GCGTC
Ksp632I 1537 GAAGAG CTCTTC(1/4)
1927 GAAGAG
MslI 40 CACGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
372 CATAGAGGTG
MvnI 1584 CGCG CG^CG
1849 CGCG
PshAI 680 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1839 GACACAGGTC
SmlI 349 CTTGAG C^T[CT][GA]AG
561 CTCAAG
StyI 682 CCAAGG C^C[AT][AT]GG
1664 CCATGG
XmnI 409 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1108 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BspMI BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I