Restriction map for
pFN21AE1349
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BalI BamHI BbeI
BbvCI BfrI BglI BglII BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SspBI SspI SwaI Tru9I
Tsp509I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 299 GGTACC G^GTACC
AccI 1364 GTCTAC GT^[AC][GT]AC
AccII 1036 CGCG CG^CG
AflIII 1368 ACACGT A^C[GA][CT]GT
Alw44I 741 GTGCAC G^TGCAC
AlwI 1138 GGATC GGATC(4/5)
ApaLI 741 GTGCAC G^TGCAC
Asp718I 299 GGTACC G^GTACC
AspI 1358 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1369 CACGTG CAC^GTG
BbsI 160 GAAGAC GAAGAC(2/6)
BcgI 18 GCAGGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 980 GTATCC GTATCC(6/5)
BclI 2 TGATCA T^GATCA
BfaI 721 CTAG C^TAG
BlpI 614 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 614 GCTCAGC GC^T[ACGT]AGC
BpuAI 160 GAAGAC GAAGAC(2/6)
BsaAI 1369 CACGTG [CT]AC^GT[GA]
BsaI 1299 GAGACC GGTCTC(1/5)
BspDI 1073 ATCGAT AT^CGAT
BssSI 957 CTCGTG CACGAG(-5/-1)
BstUI 1036 CGCG CG^CG
CelII 614 GCTCAGC GC^T[ACGT]AGC
ClaI 1073 ATCGAT AT^CGAT
EarI 1396 GAAGAG CTCTTC(1/4)
EcoRV 1096 GATATC GAT^ATC
FbaI 2 TGATCA T^GATCA
KpnI 299 GGTACC GGTAC^C
Ksp632I 1396 GAAGAG CTCTTC(1/4)
MaeI 721 CTAG C^TAG
MvnI 1036 CGCG CG^CG
NheI 720 GCTAGC G^CTAGC
NspI 1172 GCATGC [GA]CATG^[CT]
PflFI 1358 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 955 GACTC GAGTC(4/5)
PmaCI 1369 CACGTG CAC^GTG
PmlI 1369 CACGTG CAC^GTG
PshAI 163 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1047 CTGCAG CTGCA^G
SbfI 1046 CCTGCAGG CCTGCA^GG
SphI 1172 GCATGC GCATG^C
Sse8387I 1046 CCTGCAGG CCTGCA^GG
Tth111I 1358 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AlwNI 764 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1333 CAGAAGCTG
AocI 646 CCTAAGG CC^T[ACGT]AGG
1390 CCTGAGG
AvaI 714 CCCGGG C^[CT]CG[GA]G
1020 CCCGGG
BanI 299 GGTACC G^G[CT][GA]CC
345 GGCACC
BpmI 370 CTCCAG CTGGAG(16/14)
358 CTGGAG
BseRI 1318 GAGGAG GAGGAG(10/8)
1348 GAGGAG
BsgI 674 CTGCAC GTGCAG(16/14)
1218 CTGCAC
BsoBI 714 CCCGGG C^[CT]CG[GA]G
1020 CCCGGG
BsrFI 302 ACCGGC [GA]^CCGG[CT]
745 ACCGGC
BsrI 102 CCAGT ACTGG(1/-1)
763 CCAGT
BstF5I 1116 CATCC GGATG(2/0)
942 GGATG
Bsu36I 646 CCTAAGG CC^T[ACGT]AGG
1390 CCTGAGG
Cfr10I 302 ACCGGC [GA]^CCGG[CT]
745 ACCGGC
DpnII 3 GATC ^GATC
1139 GATC
DraIII 856 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1310 CACGGTGTG
Eco81I 646 CCTAAGG CC^T[ACGT]AGG
1390 CCTGAGG
FokI 1116 CATCC GGATG(9/13)
942 GGATG
HaeII 305 GGCGCT [GA]GCGC^[CT]
1211 AGCGCC
MboI 3 GATC ^GATC
1139 GATC
MslI 921 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1072 CATCGATGTG
NcoI 833 CCATGG C^CATGG
1256 CCATGG
NdeII 3 GATC ^GATC
1139 GATC
PflMI 763 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
828 CCAGACCATGG
Sau3AI 3 GATC ^GATC
1139 GATC
SfaNI 895 GATGC GCATC(5/9)
1115 GCATC
SfcI 1047 CTGCAG C^T[GA][CT]AG
1110 CTACAG
SmaI 714 CCCGGG CCC^GGG
1020 CCCGGG
StuI 374 AGGCCT AGG^CCT
545 AGGCCT
TfiI 112 GAATC G^A[AT]TC
538 GAATC
Van91I 763 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
828 CCAGACCATGG
XmaCI 714 CCCGGG C^CCGGG
1020 CCCGGG
XmaI 714 CCCGGG C^CCGGG
1020 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI ApaI AspHI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PpuMI PvuII RsaI Sau96I ScrFI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI