Restriction map for
pFN21AE5307
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI KpnI MaeI MamI
MluI MluNI MroI MscI NciI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuII RcaI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TspRI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 520 AAATTT [GA]^AATT[CT]
AfaI 702 GTAC GT^AC
AlwNI 111 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 520 AAATTT [GA]^AATT[CT]
AspHI 145 GAGCAC G[AT]GC[AT]^C
AvaI 169 CCCGAG C^[CT]CG[GA]G
BamHI 591 GGATCC G^GATCC
BanI 154 GGCGCC G^G[CT][GA]CC
BanII 667 GAGCCC G[GA]GC[CT]^C
BbeI 154 GGCGCC GGCGC^C
BbsI 127 GAAGAC GAAGAC(2/6)
BglI 133 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 127 GAAGAC GAAGAC(2/6)
BsgI 230 GTGCAG GTGCAG(16/14)
BsiEI 453 CGATCG CG[GA][CT]^CG
BsiHKAI 145 GAGCAC G[AT]GC[AT]^C
BsmI 497 GCATTC GAATGC(1/-1)
BsoBI 169 CCCGAG C^[CT]CG[GA]G
BsrFI 372 GCCGGC [GA]^CCGG[CT]
BsrI 221 ACTGG ACTGG(1/-1)
BstYI 591 GGATCC [GA]^GATC[CT]
Cfr10I 372 GCCGGC [GA]^CCGG[CT]
DraII 192 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 150 CCGCGG C^C[GA][CT]GG
EarI 314 GAAGAG CTCTTC(1/4)
EcoO109I 192 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 719 CCAAGG C^C[AT][AT]GG
HapII 373 CCGG C^CGG
HindIII 767 AAGCTT A^AGCTT
HpaI 262 GTTAAC GTT^AAC
HpaII 373 CCGG C^CGG
KasI 154 GGCGCC G^GCGCC
Ksp632I 314 GAAGAG CTCTTC(1/4)
KspI 150 CCGCGG CCGC^GG
MaeII 540 ACGT A^CGT
MaeIII 50 GTGAC ^GT[ACGT]AC
MfeI 443 CAATTG C^AATTG
MflI 591 GGATCC [GA]^GATC[CT]
MslI 717 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 373 CCGG C^CGG
MunI 443 CAATTG C^AATTG
NaeI 372 GCCGGC GCC^GGC
NarI 154 GGCGCC GG^CGCC
NgoMIV 372 GCCGGC G^CCGGC
PleI 355 GAGTC GAGTC(4/5)
PstI 650 CTGCAG CTGCA^G
PvuI 453 CGATCG CGAT^CG
RsaI 702 GTAC GT^AC
SacII 150 CCGCGG CCGC^GG
SalI 536 GTCGAC G^TCGAC
SfoI 154 GGCGCC GGC^GCC
SmlI 786 CTTGAG C^T[CT][GA]AG
StyI 719 CCAAGG C^C[AT][AT]GG
Tsp45I 50 GTGAC ^GT[GC]AC
XhoII 591 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 592 GATCC GGATC(4/5)
591 GGATC
AvaII 254 GGACC G^G[AT]CC
549 GGACC
BbvI 659 GCAGC GCAGC(8/12)
41 GCTGC
BmyI 145 GAGCAC G[AGT]GC[ACT]^C
667 GAGCCC
BpmI 596 CTCCAG CTGGAG(16/14)
117 CTGGAG
BsiYI 71 CCGCGCGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
104 CCGACGGCAGG
BslI 71 CCGCGCGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
104 CCGACGGCAGG
Bsp1286I 145 GAGCAC G[AGT]GC[ACT]^C
667 GAGCCC
BssKI 84 CCTGG ^CC[ACGT]GG
493 CCTGG
BstNI 84 CCTGG CC^[AT]GG
493 CCTGG
DdeI 683 CTCAG C^T[ACGT]AG
798 CTCAG
EcoRII 84 CCTGG ^CC[AT]GG
493 CCTGG
HaeII 113 GGCGCT [GA]GCGC^[CT]
154 GGCGCC
HgaI 52 GACGC GACGC(5/10)
130 GACGC
HincII 262 GTTAAC GT[CT]^[GA]AC
536 GTCGAC
HindII 262 GTTAAC GT[CT]^[GA]AC
536 GTCGAC
HphI 49 GGTGA GGTGA(8/7)
723 GGTGA
MvaI 84 CCTGG CC^[AT]GG
493 CCTGG
ScrFI 84 CCTGG CC^[ACGT]GG
493 CCTGG
SfaNI 212 GATGC GCATC(5/9)
30 GCATC
SfcI 635 CTGTAG C^T[GA][CT]AG
650 CTGCAG
TaqI 486 TCGA T^CGA
537 TCGA
TfiI 346 GATTC G^A[AT]TC
646 GAATC
TseI 41 GCTGC G^C[AT]GC
659 GCAGC
Tsp509I 444 AATT ^AATT
521 AATT
TthHB8I 486 TCGA T^CGA
537 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccI AccII AciI AcyI AluI
BsaHI BsaJI BseRI BsmAI BsmFI
BstF5I BstUI Cac8I CfoI Cfr13I
DpnII FauI Fnu4HI FokI HaeIII
HhaI Hin1I HinP1I HinfI ItaI
MboI MboII MnlI MseI MspA1I
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I Tru9I