Restriction map for
pFN21AE3941
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I EarI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HindIII HpaI
KpnI Ksp632I MaeI MaeIII MboII
MfeI MluI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I Tsp45I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 1060 GTCTAC GT^[AC][GT]AC
AflIII 1316 ACATGT A^C[GA][CT]GT
Alw44I 1320 GTGCAC G^TGCAC
Aor51HI 1346 AGCGCT AGC^GCT
ApaI 586 GGGCCC GGGCC^C
ApaLI 1320 GTGCAC G^TGCAC
BbeI 748 GGCGCC GGCGC^C
BciVI 985 GGATAC GTATCC(6/5)
BsaBI 855 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 580 GAGACG CGTCTC(1/5)
BspLU11I 1316 ACATGT A^CATGT
BspMI 960 ACCTGC ACCTGC(4/8)
BstAPI 66 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1511 CCACCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 331 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1228 GACAGCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1346 AGCGCT AGC^GCT
FseI 546 GGCCGGCC GGCCGG^CC
HincII 1546 GTTGAC GT[CT]^[GA]AC
HindII 1546 GTTGAC GT[CT]^[GA]AC
HinfI 385 GACTC G^A[ACGT]TC
KasI 748 GGCGCC G^GCGCC
MamI 855 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 748 GGCGCC GG^CGCC
NotI 1717 GCGGCCGC GC^GGCCGC
NspI 1316 ACATGT [GA]CATG^[CT]
PflMI 1617 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 385 GACTC GAGTC(4/5)
SacI 369 GAGCTC GAGCT^C
SfiI 117 GGCCTTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 748 GGCGCC GGC^GCC
Tsp509I 1253 AATT ^AATT
Van91I 1617 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 566 CCAAGCGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AspHI 369 GAGCTC G[AT]GC[AT]^C
1320 GTGCAC
BsgI 13 CTGCAC GTGCAG(16/14)
1100 GTGCAG
BsiHKAI 369 GAGCTC G[AT]GC[AT]^C
1320 GTGCAC
Bsp1407I 302 TGTACA T^GTACA
857 TGTACA
BsrGI 302 TGTACA T^GTACA
857 TGTACA
BssSI 292 CTCGTG CACGAG(-5/-1)
949 CTCGTG
EcoT14I 530 CCATGG C^C[AT][AT]GG
830 CCATGG
MaeII 476 ACGT A^CGT
1301 ACGT
NcoI 530 CCATGG C^CATGG
830 CCATGG
PstI 319 CTGCAG CTGCA^G
445 CTGCAG
SfcI 319 CTGCAG C^T[GA][CT]AG
445 CTGCAG
SgrAI 1581 CGCCGGCG C[GA]^CCGG[CT]G
1712 CGCCGGCG
SmaI 590 CCCGGG CCC^GGG
1024 CCCGGG
SspBI 302 TGTACA T^GTACA
857 TGTACA
StyI 530 CCATGG C^C[AT][AT]GG
830 CCATGG
XmaCI 590 CCCGGG C^CCGGG
1024 CCCGGG
XmaI 590 CCCGGG C^CCGGG
1024 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BalI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KspI MboI MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PpuMI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI StuI TaqI
TseI TspRI TthHB8I XhoII