##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanII       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeIII      HgaI        
HincII      HindII      HindIII     HpaI        HphI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        MvaI        MvnI        
NaeI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp45I      TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI           14  AAATTT               [GA]^AATT[CT]
AcyI          209  GGCGCC               G[GA]^CG[CT]C
AflIII         60  ACATGT               A^C[GA][CT]GT
AluI           89  AGCT                 AG^CT
AlwI          174  GATCC                GGATC(4/5)
ApoI           14  AAATTT               [GA]^AATT[CT]
BanI          209  GGCGCC               G^G[CT][GA]CC
BbeI          209  GGCGCC               GGCGC^C
BbvI           69  GCTGC                GCAGC(8/12)
BsaHI         209  GGCGCC               G[GA]^CG[CT]C
BsaJI          74  CCAAGG               C^C[ACGT][ACGT]GG
BseRI          43  GAGGAG               GAGGAG(10/8)
BsmFI          94  GTCCC                GGGAC(10/14)
BspLU11I       60  ACATGT               A^CATGT
BstF5I        157  GGATG                GGATG(2/0)
CfoI          210  GCGC                 GCG^C
DpnII         174  GATC                 ^GATC
EcoT14I        74  CCAAGG               C^C[AT][AT]GG
Fnu4HI         69  GCTGC                GC^[ACGT]GC
FokI          157  GGATG                GGATG(9/13)
HaeII         209  GGCGCC               [GA]GCGC^[CT]
HapII         207  CCGG                 C^CGG
HhaI          210  GCGC                 GCG^C
Hin1I         209  GGCGCC               G[GA]^CG[CT]C
HinP1I        210  GCGC                 G^CGC
HpaII         207  CCGG                 C^CGG
ItaI           69  GCTGC                GC^[ACGT]GC
KasI          209  GGCGCC               G^GCGCC
MboI          174  GATC                 ^GATC
MspI          207  CCGG                 C^CGG
MwoI          201  GCTGTTCCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI          209  GGCGCC               GG^CGCC
NdeII         174  GATC                 ^GATC
NspI           60  ACATGT               [GA]CATG^[CT]
PleI          150  GAGTC                GAGTC(4/5)
Sau3AI        174  GATC                 ^GATC
SfoI          209  GGCGCC               GGC^GCC
StyI           74  CCAAGG               C^C[AT][AT]GG
TaqI           57  TCGA                 T^CGA
TseI           69  GCTGC                G^C[AT]GC
Tsp509I        15  AATT                 ^AATT
TthHB8I        57  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

AvaII          93  GGTCC                G^G[AT]CC
              162  GGTCC               
Cfr13I         93  GGTCC                G^G[ACGT]CC
              162  GGTCC               
Sau96I         93  GGTCC                G^G[ACGT]CC
              162  GGTCC               
TfiI          118  GAATC                G^A[AT]TC
              132  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

HinfI       MnlI        NlaIII      NlaIV