##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BanII       BbeI        BbrPI       BbsI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BmyI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AciI          251  CCGC                 CCGC(-3/-1)
AfaI          435  GTAC                 GT^AC
AlwNI         394  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         113  GGACC                G^G[AT]CC
BamHI         297  GGATCC               G^GATCC
BbvCI          16  GCTGAGG              CCTCAGC(-5/-2)
BcgI           89  GCAGACTCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI           38  CTAG                 C^TAG
BlpI          259  GCTAAGC              GC^T[ACGT]AGC
BpmI           71  CTGGAG               CTGGAG(16/14)
Bpu1102I      259  GCTAAGC              GC^T[ACGT]AGC
BsmAI         150  GTCTC                GTCTC(1/5)
BsmBI         149  CGTCTC               CGTCTC(1/5)
BsrDI         417  CATTGC               GCAATG(2/0)
BstF5I        162  CATCC                GGATG(2/0)
BstXI         416  CCATTGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         297  GGATCC               [GA]^GATC[CT]
CelII         259  GCTAAGC              GC^T[ACGT]AGC
DpnII         298  GATC                 ^GATC
DsaI          189  CCATGG               C^C[GA][CT]GG
EarI           64  GAAGAG               CTCTTC(1/4)
FauI          250  CCCGC                CCCGC(4/6)
FokI          162  CATCC                GGATG(9/13)
HinfI          92  GACTC                G^A[ACGT]TC
Ksp632I        64  GAAGAG               CTCTTC(1/4)
MaeI           38  CTAG                 C^TAG
MaeII         326  ACGT                 A^CGT
MboI          298  GATC                 ^GATC
MboII          64  GAAGA                GAAGA(8/7)
MflI          297  GGATCC               [GA]^GATC[CT]
MseI          227  TTAA                 T^TAA
NcoI          189  CCATGG               C^CATGG
NdeII         298  GATC                 ^GATC
NspI          291  GCATGT               [GA]CATG^[CT]
PleI           92  GACTC                GAGTC(4/5)
RsaI          435  GTAC                 GT^AC
Sau3AI        298  GATC                 ^GATC
SfaNI         220  GATGC                GCATC(5/9)
SmlI          244  CTCAAG               C^T[CT][GA]AG
Tru9I         227  TTAA                 T^TAA
TspRI         277  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         297  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AlwI          298  GATCC                GGATC(4/5)
              297  GGATC               
BseRI          94  CTCCTC               GAGGAG(10/8)
               19  GAGGAG              
BsgI          274  CTGCAC               GTGCAG(16/14)
              379  GTGCAG              
Cac8I         249  GCCCGC               GC[ACGT]^[ACGT]GC
              381  GCAGGC              
HaeIII        186  GGCC                 GG^CC
              426  GGCC                
MwoI           41  GCCTGTGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              264  GCTGAGTGAGC         
NlaIV         113  GGACCC               GG[ACGT]^[ACGT]CC
              297  GGATCC              
Tsp45I         34  GTCAC                ^GT[GC]AC
              281  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AluI        BbvI        BsaJI       BssKI       BstNI       
Cfr13I      DdeI        EcoRII      EcoT14I     Fnu4HI      
HphI        ItaI        MaeIII      MnlI        MvaI        
NlaIII      Sau96I      ScrFI       StyI        TseI