Restriction map for
pFN21AE1540
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 328 AAATTC [GA]^AATT[CT]
AlwNI 34 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 328 AAATTC [GA]^AATT[CT]
AvaI 155 CCCGGG C^[CT]CG[GA]G
BamHI 66 GGATCC G^GATCC
BbvCI 24 GCTGAGG CCTCAGC(-5/-2)
BbvI 48 GCTGC GCAGC(8/12)
BsaJI 155 CCCGGG C^C[ACGT][ACGT]GG
BsmAI 30 GAGAC GTCTC(1/5)
BsoBI 155 CCCGGG C^[CT]CG[GA]G
BstYI 66 GGATCC [GA]^GATC[CT]
CfoI 22 GCGC GCG^C
EaeI 41 TGGCCG [CT]^GGCC[GA]
HaeII 21 GGCGCT [GA]GCGC^[CT]
HapII 156 CCGG C^CGG
HhaI 22 GCGC GCG^C
HinP1I 22 GCGC G^CGC
HinfI 352 GACTC G^A[ACGT]TC
HpaII 156 CCGG C^CGG
HphI 73 GGTGA GGTGA(8/7)
MaeIII 170 GTAAC ^GT[ACGT]AC
MflI 66 GGATCC [GA]^GATC[CT]
MspI 156 CCGG C^CGG
NlaIV 66 GGATCC GG[ACGT]^[ACGT]CC
PflMI 135 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 352 GACTC GAGTC(4/5)
PstI 49 CTGCAG CTGCA^G
SfaNI 76 GATGC GCATC(5/9)
SfcI 49 CTGCAG C^T[GA][CT]AG
SmaI 155 CCCGGG CCC^GGG
SmlI 16 CTTGAG C^T[CT][GA]AG
TseI 48 GCTGC G^C[AT]GC
TspRI 323 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 135 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 66 GGATCC [GA]^GATC[CT]
XmaCI 155 CCCGGG C^CCGGG
XmaI 155 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 4 GCGG CCGC(-3/-1)
79 GCGG
AluI 14 AGCT AG^CT
47 AGCT
BcnI 155 CCCGG CC^[GC]GG
156 CCGGG
BseRI 10 GAGGAG GAGGAG(10/8)
27 GAGGAG
BstNI 70 CCTGG CC^[AT]GG
161 CCAGG
Cac8I 39 GCTGGC GC[ACGT]^[ACGT]GC
51 GCAGGC
DpnII 67 GATC ^GATC
145 GATC
EcoRII 70 CCTGG ^CC[AT]GG
161 CCAGG
Fnu4HI 48 GCTGC GC^[ACGT]GC
79 GCGGC
ItaI 48 GCTGC GC^[ACGT]GC
79 GCGGC
MboI 67 GATC ^GATC
145 GATC
MboII 338 GAAGA GAAGA(8/7)
361 GAAGA
MvaI 70 CCTGG CC^[AT]GG
161 CCAGG
MwoI 15 GCTTGAGGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
39 GCTGGCCGAGC
NciI 155 CCCGG CC^[GC]GG
156 CCGGG
NdeII 67 GATC ^GATC
145 GATC
Sau3AI 67 GATC ^GATC
145 GATC
Tsp509I 214 AATT ^AATT
329 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AlwI BsiYI BslI BssKI Cfr13I
DdeI HaeIII MnlI MseI Sau96I
ScrFI Tru9I