Restriction map for
pFN21AB8921
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I CelII
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I EarI Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MaeII
MaeIII MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NdeII NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI Sau3AI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tru9I Tsp45I Tth111I
Van91I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 321 AAATTC [GA]^AATT[CT]
AocI 405 CCTGAGG CC^T[ACGT]AGG
ApaI 48 GGGCCC GGGCC^C
ApoI 321 AAATTC [GA]^AATT[CT]
Asp700I 288 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 67 GGACC G^G[AT]CC
BanI 88 GGCGCC G^G[CT][GA]CC
BbeI 88 GGCGCC GGCGC^C
BglI 91 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BspMI 397 ACCTGC ACCTGC(4/8)
BsrBI 335 GAGCGG CCGCTC(-3/-3)
BsrFI 195 GCCGGC [GA]^CCGG[CT]
BstAPI 508 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 767 CCACGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 405 CCTGAGG CC^T[ACGT]AGG
Cfr10I 195 GCCGGC [GA]^CCGG[CT]
DdeI 406 CTGAG C^T[ACGT]AG
DsaI 101 CCGCGG C^C[GA][CT]GG
Eco81I 405 CCTGAGG CC^T[ACGT]AGG
HinfI 758 GACTC G^A[ACGT]TC
HphI 285 GGTGA GGTGA(8/7)
KasI 88 GGCGCC G^GCGCC
KspI 101 CCGCGG CCGC^GG
MboII 328 GAAGA GAAGA(8/7)
MslI 768 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 195 GCCGGC GCC^GGC
NarI 88 GGCGCC GG^CGCC
NgoMIV 195 GCCGGC G^CCGGC
NspI 785 GCATGT [GA]CATG^[CT]
PleI 758 GACTC GAGTC(4/5)
PshAI 549 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 101 CCGCGG CCGC^GG
SbfI 687 CCTGCAGG CCTGCA^GG
SfoI 88 GGCGCC GGC^GCC
Sse8387I 687 CCTGCAGG CCTGCA^GG
TaqI 296 TCGA T^CGA
Tsp509I 322 AATT ^AATT
TthHB8I 296 TCGA T^CGA
XcmI 743 CCATCCCGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 288 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 88 GGCGCC G[GA]^CG[CT]C
183 GGCGTC
AfaI 495 GTAC GT^AC
789 GTAC
BanII 48 GGGCCC G[GA]GC[CT]^C
387 GAGCCC
BcgI 590 CGAAAAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
249 GCACGGCGCTCG
BsaHI 88 GGCGCC G[GA]^CG[CT]C
183 GGCGTC
BsgI 399 CTGCAC GTGCAG(16/14)
515 GTGCAG
BsmFI 756 GGGAC GGGAC(10/14)
186 GTCCC
BsrDI 504 CATTGC GCAATG(2/0)
20 GCAATG
BsrI 612 ACTGG ACTGG(1/-1)
772 CCAGT
DraII 47 AGGGCCC [GA]G^G[ACGT]CC[CT]
78 GGGGCCT
EcoO109I 47 AGGGCCC [GA]G^G[ACGT]CC[CT]
78 GGGGCCT
EcoT14I 176 CCAAGG C^C[AT][AT]GG
584 CCAAGG
FauI 189 CCCGC CCCGC(4/6)
747 CCCGC
Hin1I 88 GGCGCC G[GA]^CG[CT]C
183 GGCGTC
NlaIII 156 CATG CATG^
786 CATG
PstI 598 CTGCAG CTGCA^G
688 CTGCAG
RsaI 495 GTAC GT^AC
789 GTAC
SfaNI 345 GATGC GCATC(5/9)
712 GCATC
SfcI 598 CTGCAG C^T[GA][CT]AG
688 CTGCAG
SmaI 75 CCCGGG CCC^GGG
274 CCCGGG
StyI 176 CCAAGG C^C[AT][AT]GG
584 CCAAGG
XmaCI 75 CCCGGG C^CCGGG
274 CCCGGG
XmaI 75 CCCGGG C^CCGGG
274 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AvaI BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I EaeI
EagI EclXI Eco52I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV Sau96I ScrFI TseI
TspRI