Restriction map for
pFN21AE9658
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tsp45I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 576 GACGTC GACGT^C
AccI 106 GTCTAC GT^[AC][GT]AC
AcsI 289 AAATTC [GA]^AATT[CT]
AcyI 576 GACGTC G[GA]^CG[CT]C
Alw44I 448 GTGCAC G^TGCAC
ApaLI 448 GTGCAC G^TGCAC
ApoI 289 AAATTC [GA]^AATT[CT]
BalI 33 TGGCCA TGG^CCA
BamHI 512 GGATCC G^GATCC
BsaHI 576 GACGTC G[GA]^CG[CT]C
BsgI 202 CTGCAC GTGCAG(16/14)
BsmAI 348 GTCTC GTCTC(1/5)
BsrFI 150 GCCGGT [GA]^CCGG[CT]
BsrI 280 CCAGT ACTGG(1/-1)
BssHII 615 GCGCGC G^CGCGC
BssSI 453 CACGAG CACGAG(-5/-1)
Cac8I 615 GCGCGC GC[ACGT]^[ACGT]GC
Cfr10I 150 GCCGGT [GA]^CCGG[CT]
DdeI 333 CTCAG C^T[ACGT]AG
DraI 588 TTTAAA TTT^AAA
DsaI 113 CCGTGG C^C[GA][CT]GG
EarI 610 GAAGAG CTCTTC(1/4)
EcoT22I 541 ATGCAT ATGCA^T
HgaI 209 GCGTC GACGC(5/10)
Hin1I 576 GACGTC G[GA]^CG[CT]C
Ksp632I 610 GAAGAG CTCTTC(1/4)
MluNI 33 TGGCCA TGG^CCA
MscI 33 TGGCCA TGG^CCA
MseI 589 TTAA T^TAA
MslI 538 CACATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 361 CAGCTG C[AC]G^C[GT]G
MwoI 312 GCCTCCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 512 GGATCC GG[ACGT]^[ACGT]CC
NsiI 541 ATGCAT ATGCA^T
NspI 539 ACATGC [GA]CATG^[CT]
PflMI 36 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 337 GACTC GAGTC(4/5)
PvuII 361 CAGCTG CAG^CTG
SapI 610 GAAGAGC GCTCTTC(1/4)
TfiI 180 GAATC G^A[AT]TC
Tru9I 589 TTAA T^TAA
Tsp509I 290 AATT ^AATT
Van91I 36 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 467 CCACTCGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
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AccII 100 CGCG CG^CG
616 CGCG
AfaI 141 GTAC GT^AC
431 GTAC
AvaII 153 GGTCC G^G[AT]CC
411 GGACC
BpmI 67 CTGGAG CTGGAG(16/14)
223 CTGGAG
BsaAI 94 TACGTG [CT]AC^GT[GA]
604 TACGTG
BstUI 100 CGCG CG^CG
616 CGCG
BstYI 485 AGATCC [GA]^GATC[CT]
512 GGATCC
EaeI 33 TGGCCA [CT]^GGCC[GA]
44 TGGCCG
HinfI 180 GAATC G^A[ACGT]TC
337 GACTC
HphI 255 GGTGA GGTGA(8/7)
305 TCACC
MflI 485 AGATCC [GA]^GATC[CT]
512 GGATCC
MvnI 100 CGCG CG^CG
616 CGCG
RsaI 141 GTAC GT^AC
431 GTAC
XhoII 485 AGATCC [GA]^GATC[CT]
512 GGATCC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI AspHI BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
CfoI Cfr13I DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MboI MboII
MnlI MspI MvaI NciI NdeII
NlaIII Sau3AI Sau96I ScrFI SfaNI
TseI TspRI