Restriction map for
pFN21AE6342
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1286I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MroI MspA1I
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI TspRI
Tth111I Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 817 GACGTC GACGT^C
AccII 1066 CGCG CG^CG
AciI 1065 CCGC CCGC(-3/-1)
Asp700I 802 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 5 CCCGGG C^[CT]CG[GA]G
BalI 751 TGGCCA TGG^CCA
BanI 892 GGTGCC G^G[CT][GA]CC
BfaI 82 CTAG C^TAG
BglI 1033 GCCATACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 1075 GAGGAG GAGGAG(10/8)
BsgI 233 CTGCAC GTGCAG(16/14)
BsoBI 5 CCCGGG C^[CT]CG[GA]G
Bsp1407I 512 TGTACA T^GTACA
BspMI 915 GCAGGT ACCTGC(4/8)
BsrGI 512 TGTACA T^GTACA
BstEII 733 GGTGACC G^GT[ACGT]ACC
BstPI 733 GGTGACC G^GT[ACGT]ACC
BstUI 1066 CGCG CG^CG
BstXI 896 CCATTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 733 GGTGACC G^GT[ACGT]ACC
EcoRV 418 GATATC GAT^ATC
EcoT22I 33 ATGCAT ATGCA^T
FauI 1064 CCCGC CCCGC(4/6)
MaeI 82 CTAG C^TAG
MaeIII 734 GTGAC ^GT[ACGT]AC
MfeI 569 CAATTG C^AATTG
MluNI 751 TGGCCA TGG^CCA
MscI 751 TGGCCA TGG^CCA
MslI 30 CATATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 569 CAATTG C^AATTG
MvnI 1066 CGCG CG^CG
NdeI 30 CATATG CA^TATG
NsiI 33 ATGCAT ATGCA^T
NspI 35 GCATGC [GA]CATG^[CT]
PleI 127 GACTC GAGTC(4/5)
PshAI 149 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 341 CTGCAG CTGCA^G
SapI 1081 GAAGAGC GCTCTTC(1/4)
SfcI 341 CTGCAG C^T[GA][CT]AG
SmaI 5 CCCGGG CCC^GGG
SmlI 613 CTCAAG C^T[CT][GA]AG
SphI 35 GCATGC GCATG^C
SspBI 512 TGTACA T^GTACA
TaqI 640 TCGA T^CGA
Tsp45I 734 GTGAC ^GT[GC]AC
TthHB8I 640 TCGA T^CGA
XbaI 81 TCTAGA T^CTAGA
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
XmnI 802 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 76 GAATTT [GA]^AATT[CT]
877 GAATTT
AcyI 700 GACGCC G[GA]^CG[CT]C
817 GACGTC
AluI 908 AGCT AG^CT
1085 AGCT
ApoI 76 GAATTT [GA]^AATT[CT]
877 GAATTT
BclI 479 TGATCA T^GATCA
950 TGATCA
BglII 534 AGATCT A^GATCT
922 AGATCT
BpmI 766 CTCCAG CTGGAG(16/14)
904 CTGGAG
BsaHI 700 GACGCC G[GA]^CG[CT]C
817 GACGTC
BsmFI 746 GGGAC GGGAC(10/14)
627 GTCCC
BstYI 534 AGATCT [GA]^GATC[CT]
922 AGATCT
CfoI 49 GCGC GCG^C
757 GCGC
DdeI 206 CTCAG C^T[ACGT]AG
873 CTCAG
EaeI 213 TGGCCG [CT]^GGCC[GA]
751 TGGCCA
EcoT14I 1007 CCAAGG C^C[AT][AT]GG
1043 CCAAGG
FbaI 479 TGATCA T^GATCA
950 TGATCA
HaeII 48 GGCGCT [GA]GCGC^[CT]
756 AGCGCC
HgaI 700 GACGC GACGC(5/10)
858 GACGC
HhaI 49 GCGC GCG^C
757 GCGC
Hin1I 700 GACGCC G[GA]^CG[CT]C
817 GACGTC
HinP1I 49 GCGC G^CGC
757 GCGC
HphI 733 GGTGA GGTGA(8/7)
918 GGTGA
MaeII 625 ACGT A^CGT
818 ACGT
MflI 534 AGATCT [GA]^GATC[CT]
922 AGATCT
SfaNI 762 GATGC GCATC(5/9)
1030 GATGC
StyI 1007 CCAAGG C^C[AT][AT]GG
1043 CCAAGG
XhoII 534 AGATCT [GA]^GATC[CT]
922 AGATCT
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AvaII BbvI BcnI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI Ksp632I MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TfiI
Tru9I TseI Tsp509I