Restriction map for
pFN21AE3943
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraI DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HincII
HindII HindIII HinfI HpaI KpnI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
Sau3AI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI TfiI
Tru9I Tth111I Van91I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 364 GACGTC GACGT^C
AcsI 229 GAATTT [GA]^AATT[CT]
AfaI 515 GTAC GT^AC
Alw44I 379 GTGCAC G^TGCAC
ApaLI 379 GTGCAC G^TGCAC
ApoI 229 GAATTT [GA]^AATT[CT]
AvaII 535 GGTCC G^G[AT]CC
AviII 1061 TGCGCA TGC^GCA
BanI 636 GGCGCC G^G[CT][GA]CC
BbeI 636 GGCGCC GGCGC^C
BcgI 928 GCAGCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 931 GCCGCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 68 GAGACC GGTCTC(1/5)
BsgI 592 CTGCAC GTGCAG(16/14)
BsiEI 938 CGGCCG CG[GA][CT]^CG
BsmAI 68 GAGAC GTCTC(1/5)
BsrBI 343 CCGCTC CCGCTC(-3/-3)
BssSI 1114 CTCGTG CACGAG(-5/-1)
DraIII 1021 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 938 CGGCCG C^GGCCG
EarI 346 CTCTTC CTCTTC(1/4)
EclXI 938 CGGCCG C^GGCCG
Eco52I 938 CGGCCG C^GGCCG
EcoNI 987 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1061 TGCGCA TGC^GCA
HphI 1014 GGTGA GGTGA(8/7)
KasI 636 GGCGCC G^GCGCC
Ksp632I 346 CTCTTC CTCTTC(1/4)
KspI 330 CCGCGG CCGC^GG
MaeII 365 ACGT A^CGT
MaeIII 799 GTGAC ^GT[ACGT]AC
NarI 636 GGCGCC GG^CGCC
NlaIII 174 CATG CATG^
NspI 173 GCATGC [GA]CATG^[CT]
PpuMI 534 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 801 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 477 CAGCTG CAG^CTG
RsaI 515 GTAC GT^AC
SacI 487 GAGCTC GAGCT^C
SacII 330 CCGCGG CCGC^GG
SapI 345 GCTCTTC GCTCTTC(1/4)
SfoI 636 GGCGCC GGC^GCC
SmaI 300 CCCGGG CCC^GGG
SphI 173 GCATGC GCATG^C
Tsp45I 799 GTGAC ^GT[GC]AC
Tsp509I 230 AATT ^AATT
XmaCI 300 CCCGGG C^CCGGG
XmaI 300 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 87 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
96 CAGGGCCTG
BanII 487 GAGCTC G[GA]GC[CT]^C
775 GAGCCC
BfaI 520 CTAG C^TAG
598 CTAG
BseRI 28 GAGGAG GAGGAG(10/8)
961 GAGGAG
BspMI 118 ACCTGC ACCTGC(4/8)
422 ACCTGC
BstF5I 618 CATCC GGATG(2/0)
1096 CATCC
DraII 97 AGGGCCT [GA]G^G[ACGT]CC[CT]
534 GGGTCCC
DsaI 330 CCGCGG C^C[GA][CT]GG
827 CCGTGG
EcoO109I 97 AGGGCCT [GA]G^G[ACGT]CC[CT]
534 GGGTCCC
FokI 618 CATCC GGATG(9/13)
1096 CATCC
MaeI 520 CTAG C^TAG
598 CTAG
PaeR7I 550 CTCGAG C^TCGAG
916 CTCGAG
SfaNI 691 GATGC GCATC(5/9)
1095 GCATC
XhoI 550 CTCGAG C^TCGAG
916 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI BbvI BcnI BmyI BpmI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV PstI
Sau96I ScrFI SfcI SmlI StuI
TaqI TseI TspRI TthHB8I