Restriction map for
pFN21AB6530
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI AlwI
AocI ApaI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeIII HgaI Hin1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MaeI MaeII MamI
MboI MflI MluI MluNI MroI
MscI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI Sau3AI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 331 GGTACC G^GTACC
AccII 607 CGCG CG^CG
AcsI 441 GAATTT [GA]^AATT[CT]
AlwNI 357 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 441 GAATTT [GA]^AATT[CT]
Asp700I 630 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 331 GGTACC G^GTACC
BanI 331 GGTACC G^G[CT][GA]CC
BanII 72 GAGCCC G[GA]GC[CT]^C
BbsI 315 GAAGAC GAAGAC(2/6)
BcgI 609 CGATGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 528 CCGGG CC^[GC]GG
BpuAI 315 GAAGAC GAAGAC(2/6)
BsgI 210 GTGCAG GTGCAG(16/14)
BsmFI 800 GGGAC GGGAC(10/14)
BsmI 307 GAATGC GAATGC(1/-1)
Bsp1407I 265 TGTACA T^GTACA
BsrDI 25 CATTGC GCAATG(2/0)
BsrFI 173 ACCGGC [GA]^CCGG[CT]
BsrGI 265 TGTACA T^GTACA
BstAPI 563 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 607 CGCG CG^CG
Cfr10I 173 ACCGGC [GA]^CCGG[CT]
DdeI 5 CTGAG C^T[ACGT]AG
DsaI 509 CCACGG C^C[GA][CT]GG
EcoNI 60 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 245 CCAAGG C^C[AT][AT]GG
HinfI 34 GAATC G^A[ACGT]TC
HphI 506 TCACC GGTGA(8/7)
KpnI 331 GGTACC GGTAC^C
MboII 315 GAAGA GAAGA(8/7)
MfeI 404 CAATTG C^AATTG
MseI 746 TTAA T^TAA
MunI 404 CAATTG C^AATTG
MvnI 607 CGCG CG^CG
NciI 528 CCGGG CC^[GC]GG
NruI 606 TCGCGA TCG^CGA
NspI 471 ACATGC [GA]CATG^[CT]
PstI 570 CTGCAG CTGCA^G
SbfI 569 CCTGCAGG CCTGCA^GG
ScaI 287 AGTACT AGT^ACT
SfcI 570 CTGCAG C^T[GA][CT]AG
Sse8387I 569 CCTGCAGG CCTGCA^GG
SspBI 265 TGTACA T^GTACA
StyI 245 CCAAGG C^C[AT][AT]GG
TfiI 34 GAATC G^A[AT]TC
Tru9I 746 TTAA T^TAA
XcmI 396 CCAGCAGCCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 630 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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Alw44I 561 GTGCAC G^TGCAC
669 GTGCAC
Aor51HI 8 AGCGCT AGC^GCT
593 AGCGCT
ApaLI 561 GTGCAC G^TGCAC
669 GTGCAC
AvaII 171 GGACC G^G[AT]CC
252 GGACC
BsaI 97 GAGACC GGTCTC(1/5)
370 GAGACC
BseRI 67 GAGGAG GAGGAG(10/8)
121 GAGGAG
BsmAI 97 GAGAC GTCTC(1/5)
370 GAGAC
BspMI 115 ACCTGC ACCTGC(4/8)
373 ACCTGC
CfoI 9 GCGC GCG^C
594 GCGC
Cfr13I 171 GGACC G^G[ACGT]CC
252 GGACC
Eco47III 8 AGCGCT AGC^GCT
593 AGCGCT
FauI 335 CCCGC CCCGC(4/6)
63 GCGGG
HaeII 8 AGCGCT [GA]GCGC^[CT]
593 AGCGCT
HapII 174 CCGG C^CGG
528 CCGG
HhaI 9 GCGC GCG^C
594 GCGC
HinP1I 9 GCGC G^CGC
594 GCGC
HpaII 174 CCGG C^CGG
528 CCGG
MslI 178 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
293 CATCCAAGTG
MspI 174 CCGG C^CGG
528 CCGG
NlaIV 331 GGTACC GG[ACGT]^[ACGT]CC
409 GGAACC
Sau96I 171 GGACC G^G[ACGT]CC
252 GGACC
Tsp509I 405 AATT ^AATT
442 AATT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I EcoRII Fnu4HI FokI ItaI
MaeIII MnlI MspA1I MvaI MwoI
NlaIII PvuII RsaI ScrFI TseI
Tsp45I TspRI