Restriction map for
pFN21AE5717
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AccI AclI AgeI AscI
AseI AsnI AviII AvrII BbrPI
BciVI BclI BfaI BglII BlnI
BsaAI BsaBI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI MaeI MaeII
MaeIII MamI MfeI MluI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfiI SfuI
SnaBI SpeI SspBI SspI StuI
SwaI TfiI Tsp45I XbaI
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# ONE CUT ENZYMES ( 68 enzymes) #
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Acc65I 1537 GGTACC G^GTACC
AccIII 237 TCCGGA T^CCGGA
AcsI 1236 GAATTT [GA]^AATT[CT]
AcyI 687 GGCGCC G[GA]^CG[CT]C
AfaI 1538 GTAC GT^AC
AflII 1128 CTTAAG C^TTAAG
AflIII 212 ACATGT A^C[GA][CT]GT
AhdI 124 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 811 GTGCAC G^TGCAC
AocI 1269 CCTCAGG CC^T[ACGT]AGG
Aor51HI 593 AGCGCT AGC^GCT
ApaLI 811 GTGCAC G^TGCAC
ApoI 1236 GAATTT [GA]^AATT[CT]
Asp700I 591 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1537 GGTACC G^GTACC
AspEI 124 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 180 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1412 TGGCCA TGG^CCA
BamHI 536 GGATCC G^GATCC
BbeI 687 GGCGCC GGCGC^C
BbsI 1947 GTCTTC GAAGAC(2/6)
BbvCI 1254 CCTCAGC CCTCAGC(-5/-2)
BcgI 39 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1128 CTTAAG C^TTAAG
BglI 1345 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1164 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1164 GCTGAGC GC^T[ACGT]AGC
BpuAI 1947 GTCTTC GAAGAC(2/6)
BsaHI 687 GGCGCC G[GA]^CG[CT]C
BsaWI 237 TCCGGA [AT]^CCGG[AT]
BseAI 237 TCCGGA T^CCGGA
BsgI 1318 GTGCAG GTGCAG(16/14)
BsmBI 715 GAGACG CGTCTC(1/5)
BspEI 237 TCCGGA T^CCGGA
BspLU11I 212 ACATGT A^CATGT
BsrDI 260 GCAATG GCAATG(2/0)
BstXI 920 CCAGGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1269 CCTCAGG CC^T[ACGT]AGG
CelII 1164 GCTGAGC GC^T[ACGT]AGC
DraI 469 TTTAAA TTT^AAA
Eam1105I 124 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1149 GAAGAG CTCTTC(1/4)
Eco47III 593 AGCGCT AGC^GCT
Eco81I 1269 CCTCAGG CC^T[ACGT]AGG
Hin1I 687 GGCGCC G[GA]^CG[CT]C
KasI 687 GGCGCC G^GCGCC
KpnI 1537 GGTACC GGTAC^C
Ksp632I 1149 GAAGAG CTCTTC(1/4)
KspI 142 CCGCGG CCGC^GG
MluNI 1412 TGGCCA TGG^CCA
MroI 237 TCCGGA T^CCGGA
MscI 1412 TGGCCA TGG^CCA
NarI 687 GGCGCC GG^CGCC
PflFI 180 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 805 CAGCTG CAG^CTG
RsaI 1538 GTAC GT^AC
SacI 514 GAGCTC GAGCT^C
SacII 142 CCGCGG CCGC^GG
SfoI 687 GGCGCC GGC^GCC
SgrAI 90 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 946 CCCGGG CCC^GGG
SphI 432 GCATGC GCATG^C
Tsp509I 1237 AATT ^AATT
Tth111I 180 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 418 CCAGGAGTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 946 CCCGGG C^CCGGG
XmaI 946 CCCGGG C^CCGGG
XmnI 591 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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BsrBI 907 CCGCTC CCGCTC(-3/-3)
493 GAGCGG
BsrFI 91 GCCGGC [GA]^CCGG[CT]
1169 GCCGGC
BssSI 204 CACGAG CACGAG(-5/-1)
961 CACGAG
Cfr10I 91 GCCGGC [GA]^CCGG[CT]
1169 GCCGGC
EaeI 1352 TGGCCG [CT]^GGCC[GA]
1412 TGGCCA
EcoNI 366 CCTCCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1254 CCTCAGCCAGG
HphI 661 GGTGA GGTGA(8/7)
1362 GGTGA
MseI 470 TTAA T^TAA
1129 TTAA
NaeI 91 GCCGGC GCC^GGC
1169 GCCGGC
NgoMIV 91 GCCGGC G^CCGGC
1169 GCCGGC
NspI 212 ACATGT [GA]CATG^[CT]
432 GCATGC
PaeR7I 673 CTCGAG C^TCGAG
910 CTCGAG
Tru9I 470 TTAA T^TAA
1129 TTAA
XhoI 673 CTCGAG C^TCGAG
910 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmlI Sse8387I
StyI TaqI TseI TspRI TthHB8I
Van91I XhoII