Restriction map for
pFN21AE4594
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AclI AcyI AflII
AflIII AgeI ApaI AscI AseI
AsnI Asp718I AspI AvrII BbeI
BcgI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsiWI
BsmBI BspHI BspLU11I BssHII Bst1107I
BstAPI BstZ17I CelII CpoI DraI
EagI EclXI Eco52I EcoRI EcoT22I
FseI Hin1I HpaI KasI KpnI
KspI MamI MluI NarI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SbfI ScaI SfiI
SfoI SgrAI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 2744 TCCGGA T^CCGGA
Alw44I 1650 GTGCAC G^TGCAC
AocI 449 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3607 AGCGCT AGC^GCT
ApaLI 1650 GTGCAC G^TGCAC
BbrPI 3487 CACGTG CAC^GTG
BciVI 149 GTATCC GTATCC(6/5)
BclI 2756 TGATCA T^GATCA
BsaI 3289 GGTCTC GGTCTC(1/5)
BseAI 2744 TCCGGA T^CCGGA
BsiEI 1489 CGACCG CG[GA][CT]^CG
BspEI 2744 TCCGGA T^CCGGA
BsrBI 3184 GAGCGG CCGCTC(-3/-3)
BsrDI 2658 CATTGC GCAATG(2/0)
BssSI 2953 CACGAG CACGAG(-5/-1)
BstEII 2400 GGTCACC G^GT[ACGT]ACC
BstPI 2400 GGTCACC G^GT[ACGT]ACC
BstXI 2774 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 449 CCTCAGG CC^T[ACGT]AGG
DrdI 2056 GACCGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3607 AGCGCT AGC^GCT
Eco81I 449 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2400 GGTCACC G^GT[ACGT]ACC
EcoRV 1937 GATATC GAT^ATC
FbaI 2756 TGATCA T^GATCA
HaeII 3607 AGCGCT [GA]GCGC^[CT]
HincII 747 GTCGAC GT[CT]^[GA]AC
HindII 747 GTCGAC GT[CT]^[GA]AC
HindIII 1453 AAGCTT A^AGCTT
MroI 2744 TCCGGA T^CCGGA
NaeI 2440 GCCGGC GCC^GGC
NgoMIV 2440 GCCGGC G^CCGGC
PflMI 2952 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3487 CACGTG CAC^GTG
PmlI 3487 CACGTG CAC^GTG
PshAI 668 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 747 GTCGAC G^TCGAC
SapI 957 GCTCTTC GCTCTTC(1/4)
SexAI 3533 ACCAGGT A^CC[AT]GGT
SmaI 2576 CCCGGG CCC^GGG
SnaBI 458 TACGTA TAC^GTA
Van91I 2952 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2576 CCCGGG C^CCGGG
XmaI 2576 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccI 747 GTCGAC GT^[AC][GT]AC
1168 GTAGAC
AcsI 1476 GAATTT [GA]^AATT[CT]
1816 GAATTT
AhdI 1902 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3532 GACCAGGTGTC
AlwNI 3196 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3412 CAGCTGCTG
ApoI 1476 GAATTT [GA]^AATT[CT]
1816 GAATTT
Asp700I 1283 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2221 GAAAAGGTTC
AspEI 1902 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3532 GACCAGGTGTC
AviII 3090 TGCGCA TGC^GCA
3203 TGCGCA
BamHI 1094 GGATCC G^GATCC
1857 GGATCC
BsaAI 458 TACGTA [CT]AC^GT[GA]
3487 CACGTG
BsaWI 2744 TCCGGA [AT]^CCGG[AT]
2968 ACCGGA
Bsp1407I 1217 TGTACA T^GTACA
2339 TGTACA
BspDI 1267 ATCGAT AT^CGAT
3298 ATCGAT
BspMI 1654 ACCTGC ACCTGC(4/8)
2528 ACCTGC
BsrFI 2440 GCCGGC [GA]^CCGG[CT]
3407 ACCGGC
BsrGI 1217 TGTACA T^GTACA
2339 TGTACA
BstBI 961 TTCGAA TT^CGAA
2310 TTCGAA
Cfr10I 2440 GCCGGC [GA]^CCGG[CT]
3407 ACCGGC
ClaI 1267 ATCGAT AT^CGAT
3298 ATCGAT
DraIII 1372 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2953 CACGAGGTG
Eam1105I 1902 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3532 GACCAGGTGTC
FspI 3090 TGCGCA TGC^GCA
3203 TGCGCA
MfeI 632 CAATTG C^AATTG
3542 CAATTG
MunI 632 CAATTG C^AATTG
3542 CAATTG
NcoI 660 CCATGG C^CATGG
3551 CCATGG
NspV 961 TTCGAA TT^CGAA
2310 TTCGAA
SacI 2425 GAGCTC GAGCT^C
2614 GAGCTC
SfcI 144 CTACAG C^T[GA][CT]AG
2317 CTACAG
SfuI 961 TTCGAA TT^CGAA
2310 TTCGAA
SmlI 625 CTTGAG C^T[CT][GA]AG
952 CTCAAG
SspBI 1217 TGTACA T^GTACA
2339 TGTACA
XmnI 1283 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2221 GAAAAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII