Restriction map for
pFN21AE3589
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MflI MluI MluNI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 589 TCCGGA T^CCGGA
AcsI 421 GAATTC [GA]^AATT[CT]
AcyI 6 GGCGTC G[GA]^CG[CT]C
ApaI 41 GGGCCC GGGCC^C
ApoI 421 GAATTC [GA]^AATT[CT]
AvaII 117 GGACC G^G[AT]CC
BanI 61 GGCACC G^G[CT][GA]CC
BbsI 529 GAAGAC GAAGAC(2/6)
BclI 32 TGATCA T^GATCA
BfaI 304 CTAG C^TAG
BpmI 108 CTCCAG CTGGAG(16/14)
BpuAI 529 GAAGAC GAAGAC(2/6)
BsaHI 6 GGCGTC G[GA]^CG[CT]C
BsaWI 589 TCCGGA [AT]^CCGG[AT]
BseAI 589 TCCGGA T^CCGGA
BsmBI 499 GAGACG CGTCTC(1/5)
BspEI 589 TCCGGA T^CCGGA
BspMI 119 ACCTGC ACCTGC(4/8)
BsrBI 26 GAGCGG CCGCTC(-3/-3)
BstNI 549 CCTGG CC^[AT]GG
BstXI 434 CCAAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 402 TGGCCG [CT]^GGCC[GA]
EcoRI 421 GAATTC G^AATTC
EcoRII 549 CCTGG ^CC[AT]GG
FbaI 32 TGATCA T^GATCA
HgaI 7 GCGTC GACGC(5/10)
Hin1I 6 GGCGTC G[GA]^CG[CT]C
HindIII 665 AAGCTT A^AGCTT
MaeI 304 CTAG C^TAG
MaeIII 673 GTTAC ^GT[ACGT]AC
MboII 529 GAAGA GAAGA(8/7)
MroI 589 TCCGGA T^CCGGA
MspA1I 111 CAGCGG C[AC]G^C[GT]G
MvaI 549 CCTGG CC^[AT]GG
MwoI 15 GCGGCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 134 ACATGC [GA]CATG^[CT]
PpuMI 116 GGGACCT [GA]G^G[AT]CC[CT]
SfaNI 77 GCATC GCATC(5/9)
SmaI 45 CCCGGG CCC^GGG
SspI 554 AATATT AAT^ATT
TfiI 387 GATTC G^A[AT]TC
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 45 CCCGGG C^[CT]CG[GA]G
51 CTCGGG
BanII 41 GGGCCC G[GA]GC[CT]^C
48 GGGCTC
BsmAI 499 GAGAC GTCTC(1/5)
513 GAGAC
BsmFI 116 GGGAC GGGAC(10/14)
128 GGGAC
BsoBI 45 CCCGGG C^[CT]CG[GA]G
51 CTCGGG
BspHI 209 TCATGA T^CATGA
228 TCATGA
CfoI 13 GCGC GCG^C
75 GCGC
DpnII 33 GATC ^GATC
321 GATC
HhaI 13 GCGC GCG^C
75 GCGC
HinP1I 13 GCGC G^CGC
75 GCGC
HphI 30 GGTGA GGTGA(8/7)
245 TCACC
MboI 33 GATC ^GATC
321 GATC
MseI 335 TTAA T^TAA
362 TTAA
NdeII 33 GATC ^GATC
321 GATC
PleI 213 GACTC GAGTC(4/5)
407 GAGTC
RcaI 209 TCATGA T^CATGA
228 TCATGA
Sau3AI 33 GATC ^GATC
321 GATC
TaqI 92 TCGA T^CGA
323 TCGA
Tru9I 335 TTAA T^TAA
362 TTAA
Tsp509I 153 AATT ^AATT
422 AATT
TspRI 329 CAGTG CA[GC]TG[ACGT][ACGT]^
399 CAGTG
TthHB8I 92 TCGA T^CGA
323 TCGA
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstUI Cac8I
Cfr13I DraII EcoO109I EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MnlI MspI MvnI
NciI NlaIII NlaIV Sau96I ScrFI
StyI TseI