Restriction map for
pFN21AE4568
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AspI BamHI BciVI BclI BfrI
BglII BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrFI BssSI Bst1107I
BstEII BstPI BstZ17I CelII Cfr10I
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI HgaI HincII HindII
HindIII HpaI KpnI KspI MamI
MluI MroI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PflMI PinAI PmeI PshAI
Psp1406I RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 934 GACGTC GACGT^C
AccI 952 GTCTAC GT^[AC][GT]AC
AcsI 241 GAATTC [GA]^AATT[CT]
AflIII 911 ACATGT A^C[GA][CT]GT
AhdI 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1311 GGGCCC GGGCC^C
ApoI 241 GAATTC [GA]^AATT[CT]
AseI 222 ATTAAT AT^TAAT
AsnI 222 ATTAAT AT^TAAT
Asp700I 2035 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2090 TGCGCA TGC^GCA
AvrII 846 CCTAGG C^CTAGG
BbeI 18 GGCGCC GGCGC^C
BbrPI 1792 CACGTG CAC^GTG
BcgI 1191 GCAGGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 846 CCTAGG C^CTAGG
BsgI 1002 CTGCAC GTGCAG(16/14)
BsiEI 156 CGATCG CG[GA][CT]^CG
BsmBI 510 GAGACG CGTCTC(1/5)
BsmFI 1545 GGGAC GGGAC(10/14)
Bsp1407I 261 TGTACA T^GTACA
BspDI 383 ATCGAT AT^CGAT
BspLU11I 911 ACATGT A^CATGT
BspMI 1779 GCAGGT ACCTGC(4/8)
BsrGI 261 TGTACA T^GTACA
BssHII 1911 GCGCGC G^CGCGC
BstAPI 1106 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 359 TTCGAA TT^CGAA
BstXI 419 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 383 ATCGAT AT^CGAT
DrdI 1714 GACAATCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 651 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 241 GAATTC G^AATTC
FspI 2090 TGCGCA TGC^GCA
HaeII 18 GGCGCC [GA]GCGC^[CT]
KasI 18 GGCGCC G^GCGCC
MfeI 1110 CAATTG C^AATTG
MseI 223 TTAA T^TAA
MunI 1110 CAATTG C^AATTG
NarI 18 GGCGCC GG^CGCC
NspV 359 TTCGAA TT^CGAA
PaeR7I 803 CTCGAG C^TCGAG
PmaCI 1792 CACGTG CAC^GTG
PmlI 1792 CACGTG CAC^GTG
PpuMI 194 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 222 ATTAAT AT^TAAT
PvuI 156 CGATCG CGAT^CG
SacI 376 GAGCTC GAGCT^C
SfoI 18 GGCGCC GGC^GCC
SfuI 359 TTCGAA TT^CGAA
SspBI 261 TGTACA T^GTACA
StuI 1877 AGGCCT AGG^CCT
Tru9I 223 TTAA T^TAA
XcmI 1991 CCAAGGTACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 803 CTCGAG C^TCGAG
XmnI 2035 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 63 CGCG CG^CG
1912 CGCG
AcyI 18 GGCGCC G[GA]^CG[CT]C
934 GACGTC
AvaI 191 CTCGGG C^[CT]CG[GA]G
803 CTCGAG
BalI 209 TGGCCA TGG^CCA
1988 TGGCCA
BbsI 592 GAAGAC GAAGAC(2/6)
648 GAAGAC
BbvCI 1249 GCTGAGG CCTCAGC(-5/-2)
2029 GCTGAGG
BpuAI 592 GAAGAC GAAGAC(2/6)
648 GAAGAC
BsaAI 868 CACGTA [CT]AC^GT[GA]
1792 CACGTG
BsaHI 18 GGCGCC G[GA]^CG[CT]C
934 GACGTC
BsaI 658 GGTCTC GGTCTC(1/5)
823 GGTCTC
BsoBI 191 CTCGGG C^[CT]CG[GA]G
803 CTCGAG
BstUI 63 CGCG CG^CG
1912 CGCG
DraII 194 GGGTCCT [GA]G^G[ACGT]CC[CT]
1032 AGGGCCT
DsaI 1053 CCATGG C^C[GA][CT]GG
1985 CCATGG
EcoO109I 194 GGGTCCT [GA]G^G[ACGT]CC[CT]
1032 AGGGCCT
Hin1I 18 GGCGCC G[GA]^CG[CT]C
934 GACGTC
MluNI 209 TGGCCA TGG^CCA
1988 TGGCCA
MscI 209 TGGCCA TGG^CCA
1988 TGGCCA
MvnI 63 CGCG CG^CG
1912 CGCG
NcoI 1053 CCATGG C^CATGG
1985 CCATGG
NspI 863 ACATGC [GA]CATG^[CT]
911 ACATGT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI Eco81I EcoNI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII