Restriction map for
pFN21AB5859
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaJI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HapII HgaI Hin1I HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MluI MluNI MroI MscI
MspI MvaI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcsI 274 GAATTT [GA]^AATT[CT]
AflIII 487 ACACGT A^C[GA][CT]GT
ApoI 274 GAATTT [GA]^AATT[CT]
BclI 690 TGATCA T^GATCA
BmyI 222 GGGCAC G[AGT]GC[ACT]^C
BsaAI 610 TACGTA [CT]AC^GT[GA]
BsaI 1329 GAGACC GGTCTC(1/5)
BseRI 447 CTCCTC GAGGAG(10/8)
BsmAI 1329 GAGAC GTCTC(1/5)
BsmFI 194 GTCCC GGGAC(10/14)
Bsp1286I 222 GGGCAC G[AGT]GC[ACT]^C
BspHI 1028 TCATGA T^CATGA
BstXI 844 CCACTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 255 GGATCT [GA]^GATC[CT]
Cac8I 642 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 33 GCGC GCG^C
EcoNI 82 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 690 TGATCA T^GATCA
HaeIII 991 GGCC GG^CC
HhaI 33 GCGC GCG^C
HinP1I 33 GCGC G^CGC
MfeI 1226 CAATTG C^AATTG
MflI 255 GGATCT [GA]^GATC[CT]
MslI 621 CACAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1226 CAATTG C^AATTG
NdeI 26 CATATG CA^TATG
NspI 791 ACATGC [GA]CATG^[CT]
RcaI 1028 TCATGA T^CATGA
SfcI 107 CTGTAG C^T[GA][CT]AG
SnaBI 610 TACGTA TAC^GTA
TspRI 632 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 841 CCACCACTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 255 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 836 GTAC GT^AC
1287 GTAC
AlwNI 305 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1354 CAGCTGCTG
BanI 223 GGCACC G^G[CT][GA]CC
1065 GGCACC
BsgI 412 GTGCAG GTGCAG(16/14)
881 GTGCAG
BsiYI 82 CCTCAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
993 CCTTGCACGGG
BslI 82 CCTCAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
993 CCTTGCACGGG
BsrDI 806 CATTGC GCAATG(2/0)
649 GCAATG
HincII 655 GTTGAC GT[CT]^[GA]AC
719 GTCAAC
HindII 655 GTTGAC GT[CT]^[GA]AC
719 GTCAAC
HphI 130 GGTGA GGTGA(8/7)
244 GGTGA
MaeII 489 ACGT A^CGT
611 ACGT
MaeIII 383 GTGAC ^GT[ACGT]AC
526 GTCAC
MspA1I 1154 CAGCTG C[AC]G^C[GT]G
1354 CAGCTG
NlaIV 223 GGCACC GG[ACGT]^[ACGT]CC
1065 GGCACC
PleI 445 GACTC GAGTC(4/5)
818 GACTC
PvuII 1154 CAGCTG CAG^CTG
1354 CAGCTG
RsaI 836 GTAC GT^AC
1287 GTAC
TfiI 417 GAATC G^A[AT]TC
1264 GATTC
Tsp45I 383 GTGAC ^GT[GC]AC
526 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI AlwI BbvI BfaI BsrI
BstF5I DdeI DpnII Fnu4HI FokI
HindIII HinfI ItaI MaeI MboI
MboII MnlI MseI MwoI NdeII
NlaIII Sau3AI SfaNI SmlI Tru9I
TseI Tsp509I