Restriction map for
pFN21AE1484
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BfaI
BfrI BlnI BsaBI BsaWI BseAI
BsiWI BsmFI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
StuI SwaI Tsp45I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1956 GACGTC GACGT^C
AccI 753 GTCTAC GT^[AC][GT]AC
AflIII 1086 ACATGT A^C[GA][CT]GT
Alw44I 967 GTGCAC G^TGCAC
AlwI 1464 GATCC GGATC(4/5)
ApaLI 967 GTGCAC G^TGCAC
BcgI 1924 GCAAAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1830 GCCTGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1242 AGATCT A^GATCT
BlpI 1035 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1035 GCTCAGC GC^T[ACGT]AGC
BsaAI 655 TACGTA [CT]AC^GT[GA]
BsiEI 1590 CGGCCG CG[GA][CT]^CG
BsmI 617 GCATTC GAATGC(1/-1)
BspHI 1731 TCATGA T^CATGA
BspLU11I 1086 ACATGT A^CATGT
BsrDI 984 CATTGC GCAATG(2/0)
CelII 1035 GCTCAGC GC^T[ACGT]AGC
DraI 373 TTTAAA TTT^AAA
EagI 1590 CGGCCG C^GGCCG
EclXI 1590 CGGCCG C^GGCCG
Eco52I 1590 CGGCCG C^GGCCG
EcoNI 1446 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1871 GCGTC GACGC(5/10)
HphI 142 TCACC GGTGA(8/7)
MaeIII 938 GTAAC ^GT[ACGT]AC
MslI 1083 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1493 CCATGG C^CATGG
NspI 1086 ACATGT [GA]CATG^[CT]
RcaI 1731 TCATGA T^CATGA
SacI 1483 GAGCTC GAGCT^C
SapI 1690 GAAGAGC GCTCTTC(1/4)
ScaI 1610 AGTACT AGT^ACT
SmlI 1876 CTTGAG C^T[CT][GA]AG
SnaBI 655 TACGTA TAC^GTA
SspI 1438 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 1870 GGCGTC G[GA]^CG[CT]C
1956 GACGTC
AlwNI 464 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1501 CAGTTTCTG
AspI 976 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1867 GACGGCGTC
BalI 1154 TGGCCA TGG^CCA
1490 TGGCCA
BanI 531 GGTGCC G^G[CT][GA]CC
1846 GGTGCC
BanII 136 GAGCCC G[GA]GC[CT]^C
1483 GAGCTC
BbsI 766 GAAGAC GAAGAC(2/6)
1669 GAAGAC
BcnI 74 CCCGG CC^[GC]GG
744 CCGGG
BpmI 1780 CTCCAG CTGGAG(16/14)
278 CTGGAG
BpuAI 766 GAAGAC GAAGAC(2/6)
1669 GAAGAC
BsaHI 1870 GGCGTC G[GA]^CG[CT]C
1956 GACGTC
BsaI 815 GAGACC GGTCTC(1/5)
1177 GAGACC
BsmBI 1865 GAGACG CGTCTC(1/5)
1954 GAGACG
BsrFI 1511 GCCGGC [GA]^CCGG[CT]
1920 GCCGGC
BstAPI 804 GCAGTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1500 GCAGTTTCTGC
BstYI 1242 AGATCT [GA]^GATC[CT]
1463 AGATCC
Cfr10I 1511 GCCGGC [GA]^CCGG[CT]
1920 GCCGGC
DsaI 881 CCACGG C^C[GA][CT]GG
1493 CCATGG
EcoRI 572 GAATTC G^AATTC
904 GAATTC
Hin1I 1870 GGCGTC G[GA]^CG[CT]C
1956 GACGTC
MaeII 656 ACGT A^CGT
1957 ACGT
MflI 1242 AGATCT [GA]^GATC[CT]
1463 AGATCC
MluNI 1154 TGGCCA TGG^CCA
1490 TGGCCA
MscI 1154 TGGCCA TGG^CCA
1490 TGGCCA
NaeI 1511 GCCGGC GCC^GGC
1920 GCCGGC
NciI 74 CCCGG CC^[GC]GG
744 CCGGG
NgoMIV 1511 GCCGGC G^CCGGC
1920 GCCGGC
PflFI 976 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1867 GACGGCGTC
PstI 1246 CTGCAG CTGCA^G
1654 CTGCAG
PvuII 467 CAGCTG CAG^CTG
1768 CAGCTG
TfiI 323 GAATC G^A[AT]TC
1719 GAATC
Tth111I 976 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1867 GACGGCGTC
XhoII 1242 AGATCT [GA]^GATC[CT]
1463 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaII BbvI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I