Restriction map for
pFN21AE2270
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbsI BbvCI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I MaeI MaeIII MamI
MboII MfeI MluI MroI MseI
MslI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfaNI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Alw44I 152 GTGCAC G^TGCAC
ApaI 7 GGGCCC GGGCC^C
ApaLI 152 GTGCAC G^TGCAC
AspHI 152 GTGCAC G[AT]GC[AT]^C
AspI 408 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 35 TGGCCA TGG^CCA
BbrPI 291 CACGTG CAC^GTG
BcgI 236 GCAAGCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 37 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 377 AGATCT A^GATCT
BpmI 78 CTCCAG CTGGAG(16/14)
BsaAI 291 CACGTG [CT]AC^GT[GA]
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsiHKAI 152 GTGCAC G[AT]GC[AT]^C
BsmFI 83 GTCCC GGGAC(10/14)
BsmI 361 GCATTC GAATGC(1/-1)
BspLU11I 197 ACATGT A^CATGT
BspMI 219 GCAGGT ACCTGC(4/8)
BsrFI 73 GCCGGC [GA]^CCGG[CT]
BstAPI 219 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 39 CATCC GGATG(2/0)
BstYI 377 AGATCT [GA]^GATC[CT]
Cfr10I 73 GCCGGC [GA]^CCGG[CT]
DrdI 160 GACTTTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
FokI 39 CATCC GGATG(9/13)
HphI 255 GGTGA GGTGA(8/7)
KspI 68 CCGCGG CCGC^GG
MflI 377 AGATCT [GA]^GATC[CT]
MluNI 35 TGGCCA TGG^CCA
MscI 35 TGGCCA TGG^CCA
NaeI 73 GCCGGC GCC^GGC
NcoI 116 CCATGG C^CATGG
NgoMIV 73 GCCGGC G^CCGGC
NspI 197 ACATGT [GA]CATG^[CT]
PflFI 408 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 291 CACGTG CAC^GTG
PmlI 291 CACGTG CAC^GTG
PstI 217 CTGCAG CTGCA^G
SacII 68 CCGCGG CCGC^GG
SfcI 217 CTGCAG C^T[GA][CT]AG
SfiI 36 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 415 TCGA T^CGA
TspRI 155 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 408 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 415 TCGA T^CGA
XcmI 38 CCATCCTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 377 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 121 GACGCC G[GA]^CG[CT]C
433 GACGCC
AflIII 197 ACATGT A^C[GA][CT]GT
292 ACGTGT
AluI 249 AGCT AG^CT
278 AGCT
Asp700I 258 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
357 GAAAGCATTC
BanII 7 GGGCCC G[GA]GC[CT]^C
63 GAGCCC
BbvI 24 GCTGC GCAGC(8/12)
216 GCTGC
BcnI 5 CCGGG CC^[GC]GG
85 CCCGG
BsaHI 121 GACGCC G[GA]^CG[CT]C
433 GACGCC
BseRI 133 GAGGAG GAGGAG(10/8)
136 GAGGAG
BsrI 156 ACTGG ACTGG(1/-1)
80 CCAGT
DpnII 243 GATC ^GATC
378 GATC
DraII 7 GGGCCCC [GA]G^G[ACGT]CC[CT]
111 AGGCCCC
EcoO109I 7 GGGCCCC [GA]G^G[ACGT]CC[CT]
111 AGGCCCC
EcoT14I 116 CCATGG C^C[AT][AT]GG
391 CCTTGG
HgaI 121 GACGC GACGC(5/10)
433 GACGC
Hin1I 121 GACGCC G[GA]^CG[CT]C
433 GACGCC
MaeII 272 ACGT A^CGT
292 ACGT
MboI 243 GATC ^GATC
378 GATC
MspA1I 22 CCGCTG C[AC]G^C[GT]G
68 CCGCGG
NciI 5 CCGGG CC^[GC]GG
85 CCCGG
NdeII 243 GATC ^GATC
378 GATC
Sau3AI 243 GATC ^GATC
378 GATC
StyI 116 CCATGG C^C[AT][AT]GG
391 CCTTGG
TseI 24 GCTGC G^C[AT]GC
216 GCTGC
XmnI 258 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
357 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AfaI BmyI BsaJI
BsiYI BslI Bsp1286I BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DsaI EaeI EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspI MvaI MvnI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI