Restriction map for
pFN21AE1123
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AlwI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanII BbeI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstYI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoT22I FauI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MflI MluI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1095 GTATAC GT^[AC][GT]AC
AccIII 1082 TCCGGA T^CCGGA
AcsI 634 GAATTC [GA]^AATT[CT]
AcyI 148 GGCGTC G[GA]^CG[CT]C
Alw44I 682 GTGCAC G^TGCAC
AocI 107 CCTCAGG CC^T[ACGT]AGG
ApaLI 682 GTGCAC G^TGCAC
ApoI 634 GAATTC [GA]^AATT[CT]
AvaI 885 CCCGGG C^[CT]CG[GA]G
BalI 92 TGGCCA TGG^CCA
BanI 1192 GGCACC G^G[CT][GA]CC
BbrPI 58 CACGTG CAC^GTG
BclI 470 TGATCA T^GATCA
BglI 94 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 874 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 874 GCTGAGC GC^T[ACGT]AGC
BsaAI 58 CACGTG [CT]AC^GT[GA]
BsaBI 1074 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 148 GGCGTC G[GA]^CG[CT]C
BsaI 376 GAGACC GGTCTC(1/5)
BsaWI 1082 TCCGGA [AT]^CCGG[AT]
BseAI 1082 TCCGGA T^CCGGA
BsoBI 885 CCCGGG C^[CT]CG[GA]G
BspEI 1082 TCCGGA T^CCGGA
BsrBI 84 GAGCGG CCGCTC(-3/-3)
BsrDI 870 CATTGC GCAATG(2/0)
Bst1107I 1095 GTATAC GTA^TAC
BstXI 1331 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1095 GTATAC GTA^TAC
Bsu36I 107 CCTCAGG CC^T[ACGT]AGG
CelII 874 GCTGAGC GC^T[ACGT]AGC
DsaI 437 CCATGG C^C[GA][CT]GG
Eco81I 107 CCTCAGG CC^T[ACGT]AGG
EcoRI 634 GAATTC G^AATTC
EcoRV 763 GATATC GAT^ATC
FbaI 470 TGATCA T^GATCA
Hin1I 148 GGCGTC G[GA]^CG[CT]C
MaeII 59 ACGT A^CGT
MamI 1074 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 92 TGGCCA TGG^CCA
MroI 1082 TCCGGA T^CCGGA
MscI 92 TGGCCA TGG^CCA
MspA1I 1222 CAGCTG C[AC]G^C[GT]G
NcoI 437 CCATGG C^CATGG
PflMI 912 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 58 CACGTG CAC^GTG
PmlI 58 CACGTG CAC^GTG
PvuII 1222 CAGCTG CAG^CTG
SmaI 885 CCCGGG CCC^GGG
Van91I 912 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 885 CCCGGG C^CCGGG
XmaI 885 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 253 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1222 CAGCTGCTG
AseI 162 ATTAAT AT^TAAT
835 ATTAAT
AsnI 162 ATTAAT AT^TAAT
835 ATTAAT
AspHI 21 GAGCAC G[AT]GC[AT]^C
682 GTGCAC
BpmI 560 CTGGAG CTGGAG(16/14)
955 CTGGAG
BseRI 10 GAGGAG GAGGAG(10/8)
1162 GAGGAG
BsgI 943 CTGCAC GTGCAG(16/14)
1219 GTGCAG
BsiHKAI 21 GAGCAC G[AT]GC[AT]^C
682 GTGCAC
BsmAI 376 GAGAC GTCTC(1/5)
424 GAGAC
BspMI 941 ACCTGC ACCTGC(4/8)
611 GCAGGT
BsrI 220 ACTGG ACTGG(1/-1)
513 ACTGG
DraII 207 AGGACCT [GA]G^G[ACGT]CC[CT]
269 AGGTCCC
EaeI 92 TGGCCA [CT]^GGCC[GA]
1235 CGGCCA
EcoO109I 207 AGGACCT [GA]G^G[ACGT]CC[CT]
269 AGGTCCC
EcoT14I 380 CCAAGG C^C[AT][AT]GG
437 CCATGG
HgaI 33 GACGC GACGC(5/10)
149 GCGTC
MaeIII 403 GTCAC ^GT[ACGT]AC
712 GTGAC
PleI 479 GACTC GAGTC(4/5)
958 GAGTC
PpuMI 207 AGGACCT [GA]G^G[AT]CC[CT]
269 AGGTCCC
PshBI 162 ATTAAT AT^TAAT
835 ATTAAT
SapI 18 GAAGAGC GCTCTTC(1/4)
188 GAAGAGC
StyI 380 CCAAGG C^C[AT][AT]GG
437 CCATGG
TfiI 432 GAATC G^A[AT]TC
1148 GAATC
Tsp45I 403 GTCAC ^GT[GC]AC
712 GTGAC
Tsp509I 161 AATT ^AATT
635 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I TseI TspRI TthHB8I