Restriction map for
pFN21AE1409
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AocI ApoI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BbrPI BbsI
BcgI BciVI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsiEI
BsiWI BsmI BspLU11I BsrBI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI DraI DraIII DrdI EagI
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HindIII HpaI KpnI MaeII MamI
MluI MluNI MscI MseI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 584 TCCGGA T^CCGGA
AcyI 3 GGCGCC G[GA]^CG[CT]C
AgeI 437 ACCGGT A^CCGGT
AhdI 910 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 441 GTGCAC G^TGCAC
Aor51HI 159 AGCGCT AGC^GCT
ApaI 200 GGGCCC GGGCC^C
ApaLI 441 GTGCAC G^TGCAC
AscI 212 GGCGCGCC GG^CGCGCC
AspEI 910 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 441 GTGCAC G[AT]GC[AT]^C
BbeI 3 GGCGCC GGCGC^C
BbvCI 871 GCTGAGG CCTCAGC(-5/-2)
BclI 342 TGATCA T^GATCA
BlpI 317 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 317 GCTCAGC GC^T[ACGT]AGC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BseAI 584 TCCGGA T^CCGGA
BsiHKAI 441 GTGCAC G[AT]GC[AT]^C
BsmBI 927 CGTCTC CGTCTC(1/5)
BspDI 1180 ATCGAT AT^CGAT
BspEI 584 TCCGGA T^CCGGA
BspMI 303 GCAGGT ACCTGC(4/8)
BssHII 213 GCGCGC G^CGCGC
BssSI 140 CACGAG CACGAG(-5/-1)
BstYI 403 GGATCT [GA]^GATC[CT]
CelII 317 GCTCAGC GC^T[ACGT]AGC
ClaI 1180 ATCGAT AT^CGAT
EaeI 92 CGGCCA [CT]^GGCC[GA]
Eam1105I 910 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 159 AGCGCT AGC^GCT
FbaI 342 TGATCA T^GATCA
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HincII 1246 GTCAAC GT[CT]^[GA]AC
HindII 1246 GTCAAC GT[CT]^[GA]AC
HphI 723 GGTGA GGTGA(8/7)
KasI 3 GGCGCC G^GCGCC
MfeI 350 CAATTG C^AATTG
MflI 403 GGATCT [GA]^GATC[CT]
MroI 584 TCCGGA T^CCGGA
MunI 350 CAATTG C^AATTG
NarI 3 GGCGCC GG^CGCC
NcoI 173 CCATGG C^CATGG
PflMI 1060 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 437 ACCGGT A^CCGGT
PshAI 969 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 301 CTGCAG CTGCA^G
SapI 116 GCTCTTC GCTCTTC(1/4)
SfcI 301 CTGCAG C^T[GA][CT]AG
SfoI 3 GGCGCC GGC^GCC
Van91I 1060 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 403 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 1272 GATCC GGATC(4/5)
403 GGATC
BanI 3 GGCGCC G^G[CT][GA]CC
629 GGTGCC
BanII 114 GGGCTC G[GA]GC[CT]^C
200 GGGCCC
BpmI 425 CTGGAG CTGGAG(16/14)
669 CTGGAG
BsaI 850 GGTCTC GGTCTC(1/5)
1034 GGTCTC
BsaWI 437 ACCGGT [AT]^CCGG[AT]
584 TCCGGA
BsgI 537 CTGCAC GTGCAG(16/14)
618 GTGCAG
Bsp1407I 499 TGTACA T^GTACA
981 TGTACA
BspHI 339 TCATGA T^CATGA
1148 TCATGA
BsrGI 499 TGTACA T^GTACA
981 TGTACA
BsrI 1255 ACTGG ACTGG(1/-1)
191 CCAGT
BstAPI 623 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1063 GCAAATGGTGC
BstXI 635 CCAGAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
815 CCAAGTGCATGG
EcoNI 297 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1292 CCTTGGAGAGG
KspI 89 CCGCGG CCGC^GG
122 CCGCGG
NaeI 231 GCCGGC GCC^GGC
957 GCCGGC
NgoMIV 231 GCCGGC G^CCGGC
957 GCCGGC
NspI 559 GCATGT [GA]CATG^[CT]
1314 ACATGC
PvuII 320 CAGCTG CAG^CTG
1156 CAGCTG
RcaI 339 TCATGA T^CATGA
1148 TCATGA
SacII 89 CCGCGG CCGC^GG
122 CCGCGG
SspBI 499 TGTACA T^GTACA
981 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaI AvaII BbvI BcnI BfaI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XmaCI XmaI