Restriction map for
pFN21AE6405
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglI BlnI BpuAI BsaAI BsaWI
BseAI BsiWI BspEI BspHI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 31 GGCGCC G[GA]^CG[CT]C
AflIII 1058 ACATGT A^C[GA][CT]GT
BanI 31 GGCGCC G^G[CT][GA]CC
BbeI 31 GGCGCC GGCGC^C
BclI 297 TGATCA T^GATCA
BsaBI 1800 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 31 GGCGCC G[GA]^CG[CT]C
BsgI 1333 GTGCAG GTGCAG(16/14)
BsiEI 5 CGGTCG CG[GA][CT]^CG
BsmBI 1045 GAGACG CGTCTC(1/5)
Bsp1407I 809 TGTACA T^GTACA
BspDI 415 ATCGAT AT^CGAT
BspLU11I 1058 ACATGT A^CATGT
BsrBI 1168 GAGCGG CCGCTC(-3/-3)
BsrFI 461 GCCGGC [GA]^CCGG[CT]
BsrGI 809 TGTACA T^GTACA
BstXI 1508 CCAACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 461 GCCGGC [GA]^CCGG[CT]
ClaI 415 ATCGAT AT^CGAT
FbaI 297 TGATCA T^GATCA
HaeII 31 GGCGCC [GA]GCGC^[CT]
Hin1I 31 GGCGCC G[GA]^CG[CT]C
KasI 31 GGCGCC G^GCGCC
MamI 1800 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 236 TTAA T^TAA
NaeI 461 GCCGGC GCC^GGC
NarI 31 GGCGCC GG^CGCC
NcoI 1359 CCATGG C^CATGG
NgoMIV 461 GCCGGC G^CCGGC
PshAI 898 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 288 GAAGAGC GCTCTTC(1/4)
ScaI 197 AGTACT AGT^ACT
SexAI 173 ACCAGGT A^CC[AT]GGT
SfoI 31 GGCGCC GGC^GCC
SmaI 1575 CCCGGG CCC^GGG
SspBI 809 TGTACA T^GTACA
StuI 1500 AGGCCT AGG^CCT
Tru9I 236 TTAA T^TAA
Tsp45I 1071 GTCAC ^GT[GC]AC
XmaCI 1575 CCCGGG C^CCGGG
XmaI 1575 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 514 GTCTAC GT^[AC][GT]AC
1441 GTCTAC
AccII 9 CGCG CG^CG
18 CGCG
AvaI 553 CTCGGG C^[CT]CG[GA]G
1575 CCCGGG
BalI 479 TGGCCA TGG^CCA
1129 TGGCCA
BglII 48 AGATCT A^GATCT
1229 AGATCT
BlpI 468 GCTCAGC GC^T[ACGT]AGC
915 GCTTAGC
Bpu1102I 468 GCTCAGC GC^T[ACGT]AGC
915 GCTTAGC
BsaI 896 GAGACC GGTCTC(1/5)
1173 GGTCTC
BseRI 1191 CTCCTC GAGGAG(10/8)
1781 GAGGAG
BsmI 693 GAATGC GAATGC(1/-1)
936 GAATGC
BsoBI 553 CTCGGG C^[CT]CG[GA]G
1575 CCCGGG
BstUI 9 CGCG CG^CG
18 CGCG
CelII 468 GCTCAGC GC^T[ACGT]AGC
915 GCTTAGC
DsaI 593 CCGTGG C^C[GA][CT]GG
1359 CCATGG
EarI 1609 CTCTTC CTCTTC(1/4)
288 GAAGAG
HgaI 996 GACGC GACGC(5/10)
1047 GACGC
Ksp632I 1609 CTCTTC CTCTTC(1/4)
288 GAAGAG
MaeIII 854 GTTAC ^GT[ACGT]AC
1071 GTCAC
MluNI 479 TGGCCA TGG^CCA
1129 TGGCCA
MscI 479 TGGCCA TGG^CCA
1129 TGGCCA
MslI 621 CATTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1719 CATCAGTGTG
MvnI 9 CGCG CG^CG
18 CGCG
PvuII 963 CAGCTG CAG^CTG
1702 CAGCTG
SacI 799 GAGCTC GAGCT^C
1681 GAGCTC
TaqI 416 TCGA T^CGA
983 TCGA
TthHB8I 416 TCGA T^CGA
983 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
TseI Tsp509I TspRI Van91I XhoII