Restriction map for
pFN21AE5204
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbsI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
Eam1105I EarI Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI Ksp632I
MaeI MaeIII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 339 GACGCC G[GA]^CG[CT]C
AocI 415 CCTGAGG CC^T[ACGT]AGG
ApaI 411 GGGCCC GGGCC^C
AscI 253 GGCGCGCC GG^CGCGCC
AspHI 115 GTGCTC G[AT]GC[AT]^C
BanI 348 GGCACC G^G[CT][GA]CC
BbrPI 283 CACGTG CAC^GTG
BbvCI 475 GCTGAGG CCTCAGC(-5/-2)
BcgI 349 GCACCCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 413 GCCCTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 559 CTGGAG CTGGAG(16/14)
BsaAI 283 CACGTG [CT]AC^GT[GA]
BsaHI 339 GACGCC G[GA]^CG[CT]C
BseRI 451 GAGGAG GAGGAG(10/8)
BsgI 193 GTGCAG GTGCAG(16/14)
BsiHKAI 115 GTGCTC G[AT]GC[AT]^C
BsmBI 61 GAGACG CGTCTC(1/5)
BssHII 254 GCGCGC G^CGCGC
BstAPI 139 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 144 GGATG GGATG(2/0)
BstXI 328 CCAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 415 CCTGAGG CC^T[ACGT]AGG
DsaI 248 CCGCGG C^C[GA][CT]GG
EaeI 19 CGGCCG [CT]^GGCC[GA]
EagI 19 CGGCCG C^GGCCG
EclXI 19 CGGCCG C^GGCCG
Eco52I 19 CGGCCG C^GGCCG
Eco81I 415 CCTGAGG CC^T[ACGT]AGG
FokI 144 GGATG GGATG(9/13)
Hin1I 339 GACGCC G[GA]^CG[CT]C
HphI 119 TCACC GGTGA(8/7)
KspI 248 CCGCGG CCGC^GG
MaeII 284 ACGT A^CGT
MboII 616 GAAGA GAAGA(8/7)
MspA1I 248 CCGCGG C[AC]G^C[GT]G
NspI 367 ACATGC [GA]CATG^[CT]
PmaCI 283 CACGTG CAC^GTG
PmlI 283 CACGTG CAC^GTG
PpuMI 442 GGGACCC [GA]G^G[AT]CC[CT]
PstI 67 CTGCAG CTGCA^G
SacII 248 CCGCGG CCGC^GG
SfcI 67 CTGCAG C^T[GA][CT]AG
SmaI 373 CCCGGG CCC^GGG
StuI 479 AGGCCT AGG^CCT
TfiI 619 GATTC G^A[AT]TC
XmaCI 373 CCCGGG C^CCGGG
XmaI 373 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 132 GTAC GT^AC
245 GTAC
AlwNI 220 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
392 CAGACCCTG
BsaWI 498 ACCGGA [AT]^CCGG[AT]
570 ACCGGA
BsiEI 19 CGGCCG CG[GA][CT]^CG
36 CGACCG
BsmAI 28 GAGAC GTCTC(1/5)
61 GAGAC
BsrBI 16 GAGCGG CCGCTC(-3/-3)
43 GAGCGG
DdeI 416 CTGAG C^T[ACGT]AG
476 CTGAG
NlaIII 323 CATG CATG^
368 CATG
PleI 76 GACTC GAGTC(4/5)
610 GAGTC
RsaI 132 GTAC GT^AC
245 GTAC
SfaNI 145 GATGC GCATC(5/9)
157 GATGC
TspRI 149 CACTG CA[GC]TG[ACGT][ACGT]^
334 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DraII EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MnlI MspI MvaI MvnI MwoI
NciI NlaIV Sau96I ScrFI TaqI
TseI TthHB8I