Restriction map for
pFN21AE5506
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmFI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DdeI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MluI MroI MspA1I
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 82 CGCG CG^CG
Asp700I 72 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1132 GAGCTC G[AT]GC[AT]^C
AvaII 621 GGACC G^G[AT]CC
BanI 163 GGTGCC G^G[CT][GA]CC
BbvI 181 GCTGC GCAGC(8/12)
BcnI 1175 CCCGG CC^[GC]GG
BfaI 1207 CTAG C^TAG
BsaAI 175 CACGTA [CT]AC^GT[GA]
BsiHKAI 1132 GAGCTC G[AT]GC[AT]^C
BsmBI 844 GAGACG CGTCTC(1/5)
BspHI 279 TCATGA T^CATGA
BssSI 530 CACGAG CACGAG(-5/-1)
BstUI 82 CGCG CG^CG
BstXI 611 CCAAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 436 GGATCT [GA]^GATC[CT]
EarI 292 GAAGAG CTCTTC(1/4)
EcoT14I 242 CCTTGG C^C[AT][AT]GG
EcoT22I 732 ATGCAT ATGCA^T
FauI 1031 CCCGC CCCGC(4/6)
Fnu4HI 181 GCTGC GC^[ACGT]GC
HapII 1176 CCGG C^CGG
HpaII 1176 CCGG C^CGG
HphI 902 TCACC GGTGA(8/7)
ItaI 181 GCTGC GC^[ACGT]GC
Ksp632I 292 GAAGAG CTCTTC(1/4)
MaeI 1207 CTAG C^TAG
MaeIII 856 GTCAC ^GT[ACGT]AC
MfeI 44 CAATTG C^AATTG
MflI 436 GGATCT [GA]^GATC[CT]
MspI 1176 CCGG C^CGG
MunI 44 CAATTG C^AATTG
MvnI 82 CGCG CG^CG
NciI 1175 CCCGG CC^[GC]GG
NheI 1206 GCTAGC G^CTAGC
NlaIV 163 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 732 ATGCAT ATGCA^T
NspI 692 GCATGT [GA]CATG^[CT]
PleI 832 GAGTC GAGTC(4/5)
RcaI 279 TCATGA T^CATGA
SacI 1132 GAGCTC GAGCT^C
SmlI 1237 CTCAAG C^T[CT][GA]AG
StyI 242 CCTTGG C^C[AT][AT]GG
TaqI 1020 TCGA T^CGA
TseI 181 GCTGC G^C[AT]GC
Tsp45I 856 GTCAC ^GT[GC]AC
TthHB8I 1020 TCGA T^CGA
XhoII 436 GGATCT [GA]^GATC[CT]
XmnI 72 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 246 GGATC GGATC(4/5)
436 GGATC
BalI 128 TGGCCA TGG^CCA
980 TGGCCA
BanII 819 GGGCTC G[GA]GC[CT]^C
1132 GAGCTC
BciVI 156 GTATCC GTATCC(6/5)
405 GTATCC
BmyI 819 GGGCTC G[AGT]GC[ACT]^C
1132 GAGCTC
BsiYI 12 CCTGCCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1069 CCGATATCAGG
BslI 12 CCTGCCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1069 CCGATATCAGG
BsmI 521 GCATTC GAATGC(1/-1)
550 GCATTC
Bsp1286I 819 GGGCTC G[AGT]GC[ACT]^C
1132 GAGCTC
Cac8I 335 GCAGGC GC[ACGT]^[ACGT]GC
1206 GCTAGC
Cfr13I 621 GGACC G^G[ACGT]CC
1045 GGCCC
EaeI 128 TGGCCA [CT]^GGCC[GA]
980 TGGCCA
EcoRV 325 GATATC GAT^ATC
1071 GATATC
MaeII 176 ACGT A^CGT
1189 ACGT
MluNI 128 TGGCCA TGG^CCA
980 TGGCCA
MscI 128 TGGCCA TGG^CCA
980 TGGCCA
MslI 530 CACGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
877 CACCTGAATG
Sau96I 621 GGACC G^G[ACGT]CC
1045 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AcsI AluI ApoI BsaBI
BsaI BsaJI BsgI BsmAI BsrI
BssKI BstF5I BstNI DpnII EcoRII
FokI HaeIII HinfI MamI MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII Sau3AI ScrFI SfaNI
TfiI Tru9I Tsp509I TspRI