Restriction map for
pFN21AE4545
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AcsI AflII AgeI
AlwI ApoI AscI AseI AsnI
Asp700I AspI AviII AvrII BamHI
BbrPI BbvCI BciVI BclI BfaI
BfrI BlnI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CpoI DraI
DraIII EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI MaeI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1535 GGTACC G^GTACC
AclI 1612 AACGTT AA^CGTT
Alw44I 1114 GTGCAC G^TGCAC
AlwNI 1774 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1766 AGCGCT AGC^GCT
ApaLI 1114 GTGCAC G^TGCAC
Asp718I 1535 GGTACC G^GTACC
AvaI 73 CCCGGG C^[CT]CG[GA]G
BbeI 850 GGCGCC GGCGC^C
BlpI 1762 GCTGAGC GC^T[ACGT]AGC
BpmI 673 CTGGAG CTGGAG(16/14)
Bpu1102I 1762 GCTGAGC GC^T[ACGT]AGC
BsgI 1720 CTGCAC GTGCAG(16/14)
BsiEI 132 CGGCCG CG[GA][CT]^CG
BsoBI 73 CCCGGG C^[CT]CG[GA]G
BspDI 1369 ATCGAT AT^CGAT
BspHI 344 TCATGA T^CATGA
BspLU11I 511 ACATGT A^CATGT
BsrDI 1518 CATTGC GCAATG(2/0)
BstAPI 1107 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 702 GGTGACC G^GT[ACGT]ACC
BstPI 702 GGTGACC G^GT[ACGT]ACC
CelII 1762 GCTGAGC GC^T[ACGT]AGC
ClaI 1369 ATCGAT AT^CGAT
EagI 132 CGGCCG C^GGCCG
EarI 9 GAAGAG CTCTTC(1/4)
EclXI 132 CGGCCG C^GGCCG
Eco47III 1766 AGCGCT AGC^GCT
Eco52I 132 CGGCCG C^GGCCG
EcoO65I 702 GGTGACC G^GT[ACGT]ACC
KasI 850 GGCGCC G^GCGCC
KpnI 1535 GGTACC GGTAC^C
Ksp632I 9 GAAGAG CTCTTC(1/4)
KspI 1168 CCGCGG CCGC^GG
NaeI 352 GCCGGC GCC^GGC
NarI 850 GGCGCC GG^CGCC
NgoMIV 352 GCCGGC G^CCGGC
NotI 131 GCGGCCGC GC^GGCCGC
NspI 511 ACATGT [GA]CATG^[CT]
PleI 1135 GAGTC GAGTC(4/5)
Psp1406I 1612 AACGTT AA^CGTT
PstI 1100 CTGCAG CTGCA^G
PvuII 1411 CAGCTG CAG^CTG
RcaI 344 TCATGA T^CATGA
SacI 1820 GAGCTC GAGCT^C
SacII 1168 CCGCGG CCGC^GG
SapI 9 GAAGAGC GCTCTTC(1/4)
SexAI 473 ACCTGGT A^CC[AT]GGT
SfiI 106 GGCCCCGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 850 GGCGCC GGC^GCC
SmaI 73 CCCGGG CCC^GGG
TfiI 305 GAATC G^A[AT]TC
XmaCI 73 CCCGGG C^CCGGG
XmaI 73 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 736 GTCTAC GT^[AC][GT]AC
1636 GTCTAC
AcyI 850 GGCGCC G[GA]^CG[CT]C
1018 GACGCC
AhdI 613 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
705 GACCCTTTGTC
AocI 907 CCTCAGG CC^T[ACGT]AGG
1740 CCTCAGG
AspEI 613 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
705 GACCCTTTGTC
AspHI 1114 GTGCAC G[AT]GC[AT]^C
1820 GAGCTC
BalI 516 TGGCCA TGG^CCA
584 TGGCCA
BcgI 1874 GCAGCAAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1877 GCAAGGTCGTCG
BglI 107 GCCCCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
577 GCCAGCCTGGC
BsaAI 805 TACGTG [CT]AC^GT[GA]
1651 TACGTG
BsaHI 850 GGCGCC G[GA]^CG[CT]C
1018 GACGCC
BsaI 157 GAGACC GGTCTC(1/5)
1240 GGTCTC
BsiHKAI 1114 GTGCAC G[AT]GC[AT]^C
1820 GAGCTC
BsmAI 157 GAGAC GTCTC(1/5)
1241 GTCTC
BstXI 953 CCATTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1022 CCATCATCCTGG
Bsu36I 907 CCTCAGG CC^T[ACGT]AGG
1740 CCTCAGG
DrdI 1828 GACGCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1857 GACCCCAGTGTC
Eam1105I 613 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
705 GACCCTTTGTC
Eco81I 907 CCTCAGG CC^T[ACGT]AGG
1740 CCTCAGG
Hin1I 850 GGCGCC G[GA]^CG[CT]C
1018 GACGCC
HinfI 305 GAATC G^A[ACGT]TC
1135 GAGTC
MluNI 516 TGGCCA TGG^CCA
584 TGGCCA
MscI 516 TGGCCA TGG^CCA
584 TGGCCA
PflMI 1676 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1803 CCACCACTTGG
PpuMI 901 GGGTCCC [GA]G^G[AT]CC[CT]
928 GGGACCC
SfcI 30 CTATAG C^T[GA][CT]AG
1100 CTGCAG
SmlI 654 CTTGAG C^T[CT][GA]AG
877 CTCAAG
StuI 393 AGGCCT AGG^CCT
1457 AGGCCT
Van91I 1676 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1803 CCACCACTTGG
XcmI 572 CCAATGCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1748 CCAAGGGCACCATGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
ApaI AvaII BanI BanII BbsI
BbvI BcnI BglII BmyI BpuAI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII