Restriction map for
pFN21AE6724
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AcyI AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BssHII BssSI
BstAPI BstEII BstPI BstUI BstXI
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflII 49 CTTAAG C^TTAAG
AhdI 987 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1316 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 987 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 57 TGGCCA TGG^CCA
BanII 1186 GAGCTC G[GA]GC[CT]^C
BciVI 1023 GGATAC GTATCC(6/5)
BfrI 49 CTTAAG C^TTAAG
BglII 1897 AGATCT A^GATCT
BsaAI 1582 TACGTG [CT]AC^GT[GA]
BsaI 44 GAGACC GGTCTC(1/5)
BsmFI 1865 GGGAC GGGAC(10/14)
Bsp1407I 754 TGTACA T^GTACA
BspHI 1934 TCATGA T^CATGA
BspLU11I 406 ACATGT A^CATGT
BspMI 1947 ACCTGC ACCTGC(4/8)
BsrFI 1833 GCCGGT [GA]^CCGG[CT]
BsrGI 754 TGTACA T^GTACA
Bst1107I 1943 GTATAC GTA^TAC
BstBI 1910 TTCGAA TT^CGAA
BstZ17I 1943 GTATAC GTA^TAC
Cfr10I 1833 GCCGGT [GA]^CCGG[CT]
DsaI 955 CCACGG C^C[GA][CT]GG
EaeI 57 TGGCCA [CT]^GGCC[GA]
Eam1105I 987 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 426 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 872 GAATTC G^AATTC
HapII 1834 CCGG C^CGG
HincII 1669 GTTAAC GT[CT]^[GA]AC
HindII 1669 GTTAAC GT[CT]^[GA]AC
HindIII 865 AAGCTT A^AGCTT
HpaI 1669 GTTAAC GTT^AAC
HpaII 1834 CCGG C^CGG
MluNI 57 TGGCCA TGG^CCA
MscI 57 TGGCCA TGG^CCA
MspI 1834 CCGG C^CGG
NspV 1910 TTCGAA TT^CGAA
PflMI 1315 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1934 TCATGA T^CATGA
SacI 1186 GAGCTC GAGCT^C
ScaI 1756 AGTACT AGT^ACT
SfuI 1910 TTCGAA TT^CGAA
SpeI 1924 ACTAGT A^CTAGT
SspBI 754 TGTACA T^GTACA
Van91I 1315 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 567 CCACCCTGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AclI 975 AACGTT AA^CGTT
1666 AACGTT
AflIII 406 ACATGT A^C[GA][CT]GT
1583 ACGTGT
Alw44I 352 GTGCAC G^TGCAC
802 GTGCAC
ApaLI 352 GTGCAC G^TGCAC
802 GTGCAC
Asp700I 609 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1521 GAATGCCTTC
BfaI 1640 CTAG C^TAG
1925 CTAG
BsmAI 17 GAGAC GTCTC(1/5)
44 GAGAC
BsrDI 795 CATTGC GCAATG(2/0)
1961 GCAATG
BstYI 1897 AGATCT [GA]^GATC[CT]
1967 AGATCC
EcoT14I 167 CCTTGG C^C[AT][AT]GG
1095 CCAAGG
MaeI 1640 CTAG C^TAG
1925 CTAG
MflI 1897 AGATCT [GA]^GATC[CT]
1967 AGATCC
MwoI 247 GCTGAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
523 GCAGGCATAGC
NspI 406 ACATGT [GA]CATG^[CT]
1655 GCATGT
Psp1406I 975 AACGTT AA^CGTT
1666 AACGTT
SmlI 49 CTTAAG C^T[CT][GA]AG
670 CTTGAG
SspI 276 AATATT AAT^ATT
1390 AATATT
StyI 167 CCTTGG C^C[AT][AT]GG
1095 CCAAGG
XhoII 1897 AGATCT [GA]^GATC[CT]
1967 AGATCC
XmnI 609 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1521 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I