Restriction map for
pFN21AE0018
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BsaAI
BsaBI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PvuI RcaI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 280 GTCTAC GT^[AC][GT]AC
AccII 203 CGCG CG^CG
AcyI 1411 GGCGTC G[GA]^CG[CT]C
AflIII 2450 ACGTGT A^C[GA][CT]GT
AlwNI 1653 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 424 GGGCCC GGGCC^C
AseI 1732 ATTAAT AT^TAAT
AsnI 1732 ATTAAT AT^TAAT
AspHI 2049 GAGCAC G[AT]GC[AT]^C
AvaI 1385 CCCGAG C^[CT]CG[GA]G
BalI 2285 TGGCCA TGG^CCA
BanII 424 GGGCCC G[GA]GC[CT]^C
BciVI 1557 GGATAC GTATCC(6/5)
BclI 1466 TGATCA T^GATCA
BglII 657 AGATCT A^GATCT
BlpI 831 GCTTAGC GC^T[ACGT]AGC
BpmI 596 CTCCAG CTGGAG(16/14)
Bpu1102I 831 GCTTAGC GC^T[ACGT]AGC
BsaHI 1411 GGCGTC G[GA]^CG[CT]C
BsaWI 1405 ACCGGA [AT]^CCGG[AT]
BsgI 1805 GTGCAG GTGCAG(16/14)
BsiHKAI 2049 GAGCAC G[AT]GC[AT]^C
BsoBI 1385 CCCGAG C^[CT]CG[GA]G
BsrBI 2291 CCGCTC CCGCTC(-3/-3)
BssSI 1530 CACGAG CACGAG(-5/-1)
BstUI 203 CGCG CG^CG
BstXI 138 CCATAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 657 AGATCT [GA]^GATC[CT]
CelII 831 GCTTAGC GC^T[ACGT]AGC
DraII 424 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 271 GACAAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2285 TGGCCA [CT]^GGCC[GA]
EcoNI 428 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 424 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1147 GATATC GAT^ATC
FbaI 1466 TGATCA T^GATCA
Hin1I 1411 GGCGTC G[GA]^CG[CT]C
MflI 657 AGATCT [GA]^GATC[CT]
MluNI 2285 TGGCCA TGG^CCA
MscI 2285 TGGCCA TGG^CCA
MspA1I 946 CAGCTG C[AC]G^C[GT]G
MvnI 203 CGCG CG^CG
NcoI 746 CCATGG C^CATGG
NspI 1368 GCATGC [GA]CATG^[CT]
PshBI 1732 ATTAAT AT^TAAT
PvuII 946 CAGCTG CAG^CTG
SapI 804 GAAGAGC GCTCTTC(1/4)
ScaI 2423 AGTACT AGT^ACT
SexAI 515 ACCAGGT A^CC[AT]GGT
SpeI 171 ACTAGT A^CTAGT
SphI 1368 GCATGC GCATG^C
Tsp45I 1655 GTGAC ^GT[GC]AC
XhoII 657 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AclI 1003 AACGTT AA^CGTT
1012 AACGTT
AlwI 530 GGATC GGATC(4/5)
2240 GGATC
AocI 85 CCTAAGG CC^T[ACGT]AGG
432 CCTGAGG
Asp700I 1701 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2435 GAATGGATTC
AvaII 148 GGACC G^G[AT]CC
1041 GGTCC
BcnI 527 CCCGG CC^[GC]GG
1548 CCGGG
BmyI 424 GGGCCC G[AGT]GC[ACT]^C
2049 GAGCAC
BsmI 158 GCATTC GAATGC(1/-1)
1195 GCATTC
Bsp1286I 424 GGGCCC G[AGT]GC[ACT]^C
2049 GAGCAC
Bsu36I 85 CCTAAGG CC^T[ACGT]AGG
432 CCTGAGG
DsaI 746 CCATGG C^C[GA][CT]GG
983 CCGTGG
Eco81I 85 CCTAAGG CC^T[ACGT]AGG
432 CCTGAGG
EcoRI 692 GAATTC G^AATTC
1131 GAATTC
EcoT14I 638 CCAAGG C^C[AT][AT]GG
746 CCATGG
FauI 1897 CCCGC CCCGC(4/6)
41 GCGGG
MslI 1268 CATACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2521 CATGTATATG
NciI 527 CCCGG CC^[GC]GG
1548 CCGGG
NdeI 248 CATATG CA^TATG
1925 CATATG
Psp1406I 1003 AACGTT AA^CGTT
1012 AACGTT
PstI 1696 CTGCAG CTGCA^G
2233 CTGCAG
StyI 638 CCAAGG C^C[AT][AT]GG
746 CCATGG
XbaI 1932 TCTAGA T^CTAGA
2295 TCTAGA
XcmI 770 CCAAAGAGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2477 CCATGAAAAATATGG
XmnI 1701 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2435 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BfaI BpuAI BsaI
BsaJI BsiYI BslI BsmAI BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I