Restriction map for
pFN21AE1546
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr13I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 673 CGCG CG^CG
AcsI 574 AAATTC [GA]^AATT[CT]
AcyI 199 GGCGCC G[GA]^CG[CT]C
AfaI 514 GTAC GT^AC
ApoI 574 AAATTC [GA]^AATT[CT]
BanII 409 GAGCCC G[GA]GC[CT]^C
BbeI 199 GGCGCC GGCGC^C
BbsI 562 GTCTTC GAAGAC(2/6)
BcnI 71 CCGGG CC^[GC]GG
BmyI 409 GAGCCC G[AGT]GC[ACT]^C
BpuAI 562 GTCTTC GAAGAC(2/6)
BsaHI 199 GGCGCC G[GA]^CG[CT]C
BsgI 244 GTGCAG GTGCAG(16/14)
Bsp1286I 409 GAGCCC G[AGT]GC[ACT]^C
BspMI 35 ACCTGC ACCTGC(4/8)
BsrDI 606 CATTGC GCAATG(2/0)
BsrI 255 CCAGT ACTGG(1/-1)
BstAPI 17 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 673 CGCG CG^CG
DraIII 18 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 611 CCGTGG C^C[GA][CT]GG
EaeI 317 CGGCCA [CT]^GGCC[GA]
EcoT14I 401 CCAAGG C^C[AT][AT]GG
HaeII 199 GGCGCC [GA]GCGC^[CT]
HaeIII 318 GGCC GG^CC
Hin1I 199 GGCGCC G[GA]^CG[CT]C
KasI 199 GGCGCC G^GCGCC
MaeII 512 ACGT A^CGT
MspA1I 415 CAGCGG C[AC]G^C[GT]G
MvnI 673 CGCG CG^CG
NaeI 196 GCCGGC GCC^GGC
NarI 199 GGCGCC GG^CGCC
NciI 71 CCGGG CC^[GC]GG
NgoMIV 196 GCCGGC G^CCGGC
NspI 260 GCATGC [GA]CATG^[CT]
RsaI 514 GTAC GT^AC
SfoI 199 GGCGCC GGC^GCC
SphI 260 GCATGC GCATG^C
StyI 401 CCAAGG C^C[AT][AT]GG
TfiI 107 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 16 GGCACC G^G[CT][GA]CC
199 GGCGCC
BsmFI 267 GGGAC GGGAC(10/14)
252 GTCCC
BspHI 206 TCATGA T^CATGA
617 TCATGA
BsrFI 3 GCCGGT [GA]^CCGG[CT]
196 GCCGGC
BstNI 225 CCTGG CC^[AT]GG
363 CCAGG
CfoI 200 GCGC GCG^C
590 GCGC
Cfr10I 3 GCCGGT [GA]^CCGG[CT]
196 GCCGGC
DdeI 116 CTCAG C^T[ACGT]AG
489 CTCAG
EcoRII 225 CCTGG ^CC[AT]GG
363 CCAGG
FauI 236 GCGGG CCCGC(4/6)
417 GCGGG
HhaI 200 GCGC GCG^C
590 GCGC
HinP1I 200 GCGC G^CGC
590 GCGC
MseI 50 TTAA T^TAA
353 TTAA
MslI 337 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
606 CATTGCCGTG
MvaI 225 CCTGG CC^[AT]GG
363 CCAGG
RcaI 206 TCATGA T^CATGA
617 TCATGA
Tru9I 50 TTAA T^TAA
353 TTAA
Tsp509I 157 AATT ^AATT
575 AATT
TspRI 256 CAGTG CA[GC]TG[ACGT][ACGT]^
593 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BssKI BstF5I Cac8I DpnII
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MspI MwoI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI SfaNI TaqI
TseI TthHB8I