##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       Hin1I       HincII      
HindII      HinfI       HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MboI        
MboII       MfeI        MflI        MluI        MroI        
MslI        MunI        NarI        NcoI        NdeI        
NdeII       NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        StyI        
SwaI        TfiI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          152  GAATTC               [GA]^AATT[CT]
AluI          249  AGCT                 AG^CT
AlwNI         139  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          152  GAATTC               [GA]^AATT[CT]
AvaII         196  GGACC                G^G[AT]CC
BalI          110  TGGCCA               TGG^CCA
BsmFI         189  GTCCC                GGGAC(10/14)
BsrFI          86  GCCGGC               [GA]^CCGG[CT]
BsrI          129  CCAGT                ACTGG(1/-1)
BssHII         90  GCGCGC               G^CGCGC
BstAPI        138  GCAGACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI          72  CCTGG                CC^[AT]GG
Cfr10I         86  GCCGGC               [GA]^CCGG[CT]
DsaI          107  CCGTGG               C^C[GA][CT]GG
EcoRI         152  GAATTC               G^AATTC
EcoRII         72  CCTGG                ^CC[AT]GG
FauI            5  CCCGC                CCCGC(4/6)
HgaI          103  GCGTC                GACGC(5/10)
HindIII       248  AAGCTT               A^AGCTT
HphI          156  TCACC                GGTGA(8/7)
MaeII         182  ACGT                 A^CGT
MaeIII        308  GTGAC                ^GT[ACGT]AC
MluNI         110  TGGCCA               TGG^CCA
MnlI          255  CCTC                 CCTC(7/6)
MscI          110  TGGCCA               TGG^CCA
MseI          278  TTAA                 T^TAA
MspA1I         80  CCGCTG               C[AC]G^C[GT]G
MvaI           72  CCTGG                CC^[AT]GG
NaeI           86  GCCGGC               GCC^GGC
NgoMIV         86  GCCGGC               G^CCGGC
PstI          145  CTGCAG               CTGCA^G
SbfI          144  CCTGCAGG             CCTGCA^GG
SfcI          145  CTGCAG               C^T[GA][CT]AG
SmlI          232  CTCAAG               C^T[CT][GA]AG
Sse8387I      144  CCTGCAGG             CCTGCA^GG
TaqI          290  TCGA                 T^CGA
Tru9I         278  TTAA                 T^TAA
Tsp45I        308  GTGAC                ^GT[GC]AC
Tsp509I       153  AATT                 ^AATT
TthHB8I       290  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AccII           7  CGCG                 CG^CG
               91  CGCG                
BcnI           38  CCCGG                CC^[GC]GG
              193  CCCGG               
BstF5I        168  CATCC                GGATG(2/0)
              266  CATCC               
BstUI           7  CGCG                 CG^CG
               91  CGCG                
Cfr13I          3  GGCCC                G^G[ACGT]CC
              196  GGACC               
EaeI           84  TGGCCG               [CT]^GGCC[GA]
              110  TGGCCA              
FokI          168  CATCC                GGATG(9/13)
              266  CATCC               
MvnI            7  CGCG                 CG^CG
               91  CGCG                
NciI           38  CCCGG                CC^[GC]GG
              193  CCCGG               
NlaIV          41  GGCTCC               GG[ACGT]^[ACGT]CC
              196  GGACCC              
Sau96I          3  GGCCC                G^G[ACGT]CC
              196  GGACC               
SfaNI         297  GATGC                GCATC(5/9)
              265  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsiYI       BslI        
BssKI       Cac8I       CfoI        Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MspI        MwoI        ScrFI       TseI        TspRI