Restriction map for
pFN21AE2221
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI Alw44I AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BanI BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI Bsu36I
CelII Cfr10I CpoI DraI DraII
DraIII DrdI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp509I Tth111I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 669 GTATAC GT^[AC][GT]AC
AccII 902 CGCG CG^CG
AflIII 701 ACATGT A^C[GA][CT]GT
AhdI 549 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 384 AGCGCT AGC^GCT
Asp700I 774 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 549 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 800 GAGCTC G[AT]GC[AT]^C
AvaI 641 CTCGAG C^[CT]CG[GA]G
BbsI 132 GTCTTC GAAGAC(2/6)
BbvCI 100 CCTCAGC CCTCAGC(-5/-2)
BfaI 952 CTAG C^TAG
BpmI 406 CTCCAG CTGGAG(16/14)
BpuAI 132 GTCTTC GAAGAC(2/6)
BsgI 85 CTGCAC GTGCAG(16/14)
BsiHKAI 800 GAGCTC G[AT]GC[AT]^C
BsiYI 655 CCAGTCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 655 CCAGTCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 975 GAATGC GAATGC(1/-1)
BsoBI 641 CTCGAG C^[CT]CG[GA]G
Bsp1407I 884 TGTACA T^GTACA
BspDI 488 ATCGAT AT^CGAT
BspHI 435 TCATGA T^CATGA
BspLU11I 701 ACATGT A^CATGT
BspMI 83 ACCTGC ACCTGC(4/8)
BsrGI 884 TGTACA T^GTACA
BssKI 45 CCAGG ^CC[ACGT]GG
Bst1107I 669 GTATAC GTA^TAC
BstNI 45 CCAGG CC^[AT]GG
BstUI 902 CGCG CG^CG
BstXI 371 CCAGCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 669 GTATAC GTA^TAC
ClaI 488 ATCGAT AT^CGAT
EaeI 59 TGGCCG [CT]^GGCC[GA]
Eam1105I 549 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 384 AGCGCT AGC^GCT
EcoRII 45 CCAGG ^CC[AT]GG
EcoT14I 962 CCATGG C^C[AT][AT]GG
EcoT22I 977 ATGCAT ATGCA^T
KspI 901 CCGCGG CCGC^GG
MaeI 952 CTAG C^TAG
MslI 436 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 901 CCGCGG C[AC]G^C[GT]G
MvaI 45 CCAGG CC^[AT]GG
MvnI 902 CGCG CG^CG
NcoI 962 CCATGG C^CATGG
NsiI 977 ATGCAT ATGCA^T
NspI 701 ACATGT [GA]CATG^[CT]
PaeR7I 641 CTCGAG C^TCGAG
PflMI 655 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 435 TCATGA T^CATGA
SacI 800 GAGCTC GAGCT^C
SacII 901 CCGCGG CCGC^GG
ScrFI 45 CCAGG CC^[ACGT]GG
SfcI 471 CTACAG C^T[GA][CT]AG
SspBI 884 TGTACA T^GTACA
StyI 962 CCATGG C^C[AT][AT]GG
Van91I 655 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 641 CTCGAG C^TCGAG
XmnI 774 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AvaII 42 GGTCC G^G[AT]CC
905 GGTCC
BanII 403 GGGCTC G[GA]GC[CT]^C
800 GAGCTC
BsaI 339 GAGACC GGTCTC(1/5)
646 GGTCTC
BsaJI 901 CCGCGG C^C[ACGT][ACGT]GG
962 CCATGG
BsmFI 159 GGGAC GGGAC(10/14)
698 GGGAC
BsrI 251 ACTGG ACTGG(1/-1)
655 CCAGT
DsaI 901 CCGCGG C^C[GA][CT]GG
962 CCATGG
HaeII 384 AGCGCT [GA]GCGC^[CT]
788 GGCGCT
SfaNI 374 GCATC GCATC(5/9)
396 GCATC
SmlI 279 CTTGAG C^T[CT][GA]AG
641 CTCGAG
TfiI 486 GAATC G^A[AT]TC
918 GATTC
Tsp45I 329 GTGAC ^GT[GC]AC
600 GTCAC
TspRI 327 CAGTG CA[GC]TG[ACGT][ACGT]^
911 CAGTG
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI BamHI
BbvI BmyI BsmAI Bsp1286I BstF5I
BstYI Cac8I CfoI Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeIII
MboI MboII MflI MnlI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I TaqI TseI TthHB8I
XhoII