Restriction map for
pFN21AE1347
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HapII Hin1I HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Asp700I 238 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 332 CCCGAG C^[CT]CG[GA]G
AvrII 757 CCTAGG C^CTAGG
BanII 833 GAGCCC G[GA]GC[CT]^C
BbsI 1021 GAAGAC GAAGAC(2/6)
BciVI 1093 GTATCC GTATCC(6/5)
BlnI 757 CCTAGG C^CTAGG
BpuAI 1021 GAAGAC GAAGAC(2/6)
BsaI 307 GGTCTC GGTCTC(1/5)
BsgI 61 GTGCAG GTGCAG(16/14)
BsoBI 332 CCCGAG C^[CT]CG[GA]G
Bsp1407I 459 TGTACA T^GTACA
BspMI 53 ACCTGC ACCTGC(4/8)
BsrGI 459 TGTACA T^GTACA
BssKI 825 CCTGG ^CC[ACGT]GG
BstF5I 792 GGATG GGATG(2/0)
BstNI 825 CCTGG CC^[AT]GG
CfoI 399 GCGC GCG^C
EagI 14 CGGCCG C^GGCCG
EarI 1009 GAAGAG CTCTTC(1/4)
EclXI 14 CGGCCG C^GGCCG
Eco52I 14 CGGCCG C^GGCCG
EcoRII 825 CCTGG ^CC[AT]GG
EcoT14I 757 CCTAGG C^C[AT][AT]GG
FokI 792 GGATG GGATG(9/13)
HaeII 398 GGCGCT [GA]GCGC^[CT]
HgaI 66 GACGC GACGC(5/10)
HhaI 399 GCGC GCG^C
HinP1I 399 GCGC G^CGC
HincII 404 GTCAAC GT[CT]^[GA]AC
HindII 404 GTCAAC GT[CT]^[GA]AC
HindIII 1063 AAGCTT A^AGCTT
Ksp632I 1009 GAAGAG CTCTTC(1/4)
MaeIII 477 GTCAC ^GT[ACGT]AC
MvaI 825 CCTGG CC^[AT]GG
NlaIII 604 CATG CATG^
NotI 13 GCGGCCGC GC^GGCCGC
PflMI 424 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 116 CAGCTG CAG^CTG
ScrFI 825 CCTGG CC^[ACGT]GG
SspBI 459 TGTACA T^GTACA
StyI 757 CCTAGG C^C[AT][AT]GG
TfiI 697 GATTC G^A[AT]TC
Tsp45I 477 GTCAC ^GT[GC]AC
Van91I 424 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 238 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcsI 238 GAATTT [GA]^AATT[CT]
324 GAATTT
Alw44I 440 GTGCAC G^TGCAC
497 GTGCAC
AlwNI 64 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
396 CAGGCGCTG
ApaLI 440 GTGCAC G^TGCAC
497 GTGCAC
ApoI 238 GAATTT [GA]^AATT[CT]
324 GAATTT
AspHI 440 GTGCAC G[AT]GC[AT]^C
497 GTGCAC
BclI 392 TGATCA T^GATCA
896 TGATCA
BglII 885 AGATCT A^GATCT
1083 AGATCT
BpmI 70 CTGGAG CTGGAG(16/14)
932 CTGGAG
BseRI 31 GAGGAG GAGGAG(10/8)
136 GAGGAG
BsiEI 14 CGGCCG CG[GA][CT]^CG
210 CGGTCG
BsiHKAI 440 GTGCAC G[AT]GC[AT]^C
497 GTGCAC
BsmAI 308 GTCTC GTCTC(1/5)
556 GTCTC
BsmFI 50 GGGAC GGGAC(10/14)
600 GTCCC
Cac8I 661 GCTGGC GC[ACGT]^[ACGT]GC
765 GCAAGC
DraI 195 TTTAAA TTT^AAA
843 TTTAAA
DraII 50 GGGACCT [GA]G^G[ACGT]CC[CT]
83 GGGTCCT
EaeI 14 CGGCCG [CT]^GGCC[GA]
26 TGGCCG
EcoO109I 50 GGGACCT [GA]G^G[ACGT]CC[CT]
83 GGGTCCT
FbaI 392 TGATCA T^GATCA
896 TGATCA
HphI 1006 GGTGA GGTGA(8/7)
478 TCACC
MspA1I 78 CAGCGG C[AC]G^C[GT]G
116 CAGCTG
PleI 815 GACTC GAGTC(4/5)
850 GACTC
PpuMI 50 GGGACCT [GA]G^G[AT]CC[CT]
83 GGGTCCT
SfaNI 793 GATGC GCATC(5/9)
95 GCATC
TaqI 311 TCGA T^CGA
318 TCGA
TthHB8I 311 TCGA T^CGA
318 TCGA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BstYI Cfr13I
DdeI DpnII FauI Fnu4HI HaeIII
HinfI ItaI MaeI MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIV RsaI Sau3AI Sau96I Tru9I
TseI Tsp509I TspRI XhoII