Restriction map for
pFN21AE4559
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbeI
BbrPI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsgI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III EcoNI EcoRI EcoRV
EcoT22I FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MamI MfeI MluI MroI MseI
MslI MunI NarI NcoI NdeI
NheI NruI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SapI ScaI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI TfiI Tru9I
Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AspI 851 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 767 GGATCC G^GATCC
BbsI 1343 GTCTTC GAAGAC(2/6)
BbvCI 1991 CCTCAGC CCTCAGC(-5/-2)
BcgI 1602 CGAACAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1741 GGATAC GTATCC(6/5)
BclI 1595 TGATCA T^GATCA
BlpI 1154 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1154 GCTCAGC GC^T[ACGT]AGC
BpuAI 1343 GTCTTC GAAGAC(2/6)
BseRI 1802 GAGGAG GAGGAG(10/8)
BsmI 560 GAATGC GAATGC(1/-1)
BspMI 1330 GCAGGT ACCTGC(4/8)
BssHII 12 GCGCGC G^CGCGC
BstXI 1373 CCAGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1154 GCTCAGC GC^T[ACGT]AGC
EagI 62 CGGCCG C^GGCCG
EclXI 62 CGGCCG C^GGCCG
Eco52I 62 CGGCCG C^GGCCG
FbaI 1595 TGATCA T^GATCA
FseI 1077 GGCCGGCC GGCCGG^CC
MaeII 1274 ACGT A^CGT
MboII 1344 TCTTC GAAGA(8/7)
NotI 61 GCGGCCGC GC^GGCCGC
PaeR7I 1727 CTCGAG C^TCGAG
PflFI 851 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1673 CAGCTG CAG^CTG
SbfI 1497 CCTGCAGG CCTGCA^GG
SexAI 852 ACCTGGT A^CC[AT]GGT
SmlI 1727 CTCGAG C^T[CT][GA]AG
Sse8387I 1497 CCTGCAGG CCTGCA^GG
StuI 351 AGGCCT AGG^CCT
Tsp509I 1608 AATT ^AATT
Tth111I 851 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1754 CCACTGGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1727 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 1099 GGCGTC G[GA]^CG[CT]C
1504 GGCGTC
AocI 1309 CCTAAGG CC^T[ACGT]AGG
1629 CCTAAGG
AspHI 974 GTGCTC G[AT]GC[AT]^C
1907 GTGCTC
BalI 945 TGGCCA TGG^CCA
1143 TGGCCA
BsaHI 1099 GGCGTC G[GA]^CG[CT]C
1504 GGCGTC
BsiEI 62 CGGCCG CG[GA][CT]^CG
1281 CGACCG
BsiHKAI 974 GTGCTC G[AT]GC[AT]^C
1907 GTGCTC
BsrFI 655 GCCGGC [GA]^CCGG[CT]
1078 GCCGGC
BstF5I 252 GGATG GGATG(2/0)
1592 GGATG
Bsu36I 1309 CCTAAGG CC^T[ACGT]AGG
1629 CCTAAGG
Cfr10I 655 GCCGGC [GA]^CCGG[CT]
1078 GCCGGC
Eco81I 1309 CCTAAGG CC^T[ACGT]AGG
1629 CCTAAGG
FokI 252 GGATG GGATG(9/13)
1592 GGATG
HgaI 1100 GCGTC GACGC(5/10)
1505 GCGTC
Hin1I 1099 GGCGTC G[GA]^CG[CT]C
1504 GGCGTC
MluNI 945 TGGCCA TGG^CCA
1143 TGGCCA
MscI 945 TGGCCA TGG^CCA
1143 TGGCCA
NaeI 655 GCCGGC GCC^GGC
1078 GCCGGC
NgoMIV 655 GCCGGC G^CCGGC
1078 GCCGGC
NspI 902 GCATGC [GA]CATG^[CT]
1118 GCATGC
PshAI 733 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1282 GACCGGGGTC
PstI 679 CTGCAG CTGCA^G
1498 CTGCAG
SfcI 679 CTGCAG C^T[GA][CT]AG
1498 CTGCAG
SphI 902 GCATGC GCATG^C
1118 GCATGC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstEII BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KspI MaeIII
MboI MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI SacII
Sau3AI Sau96I ScrFI SfaNI SfiI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII XmaCI XmaI