Restriction map for
pFN21AE1343
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MaeI MaeII
MamI MluI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 651 GGTACC G^GTACC
AccIII 761 TCCGGA T^CCGGA
AciI 627 CCGC CCGC(-3/-1)
AcsI 250 GAATTT [GA]^AATT[CT]
AlwI 324 GATCC GGATC(4/5)
ApoI 250 GAATTT [GA]^AATT[CT]
Asp718I 651 GGTACC G^GTACC
AvaI 230 CTCGGG C^[CT]CG[GA]G
AvaII 195 GGACC G^G[AT]CC
BalI 103 TGGCCA TGG^CCA
BanII 263 GGGCTC G[GA]GC[CT]^C
BglII 675 AGATCT A^GATCT
BmyI 263 GGGCTC G[AGT]GC[ACT]^C
BsaWI 761 TCCGGA [AT]^CCGG[AT]
BseAI 761 TCCGGA T^CCGGA
BsgI 133 CTGCAC GTGCAG(16/14)
BsmAI 526 GTCTC GTCTC(1/5)
BsoBI 230 CTCGGG C^[CT]CG[GA]G
Bsp1286I 263 GGGCTC G[AGT]GC[ACT]^C
BspEI 761 TCCGGA T^CCGGA
BspHI 6 TCATGA T^CATGA
BspMI 113 ACCTGC ACCTGC(4/8)
BstYI 675 AGATCT [GA]^GATC[CT]
CfoI 28 GCGC GCG^C
DraIII 136 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 103 TGGCCA [CT]^GGCC[GA]
EarI 454 CTCTTC CTCTTC(1/4)
EcoT14I 18 CCAAGG C^C[AT][AT]GG
HhaI 28 GCGC GCG^C
HinP1I 28 GCGC G^CGC
HphI 778 GGTGA GGTGA(8/7)
KpnI 651 GGTACC GGTAC^C
Ksp632I 454 CTCTTC CTCTTC(1/4)
MfeI 638 CAATTG C^AATTG
MflI 675 AGATCT [GA]^GATC[CT]
MluNI 103 TGGCCA TGG^CCA
MroI 761 TCCGGA T^CCGGA
MscI 103 TGGCCA TGG^CCA
MseI 568 TTAA T^TAA
MspA1I 530 CAGCTG C[AC]G^C[GT]G
MunI 638 CAATTG C^AATTG
PvuII 530 CAGCTG CAG^CTG
RcaI 6 TCATGA T^CATGA
SfcI 34 CTACAG C^T[GA][CT]AG
StyI 18 CCAAGG C^C[AT][AT]GG
Tru9I 568 TTAA T^TAA
XhoII 675 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 144 GTAC GT^AC
652 GTAC
BanI 234 GGTGCC G^G[CT][GA]CC
651 GGTACC
BcnI 360 CCCGG CC^[GC]GG
656 CCCGG
BpmI 409 CTGGAG CTGGAG(16/14)
682 CTGGAG
BstF5I 153 GGATG GGATG(2/0)
320 GGATG
BstXI 46 CCACTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
425 CCAACACTTTGG
Cac8I 242 GCCTGC GC[ACGT]^[ACGT]GC
419 GCAAGC
DpnII 324 GATC ^GATC
676 GATC
EcoNI 499 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
769 CCTTGCTCAGG
FokI 153 GGATG GGATG(9/13)
320 GGATG
MboI 324 GATC ^GATC
676 GATC
NciI 360 CCCGG CC^[GC]GG
656 CCCGG
NdeII 324 GATC ^GATC
676 GATC
NlaIII 7 CATG CATG^
465 CATG
NlaIV 234 GGTGCC GG[ACGT]^[ACGT]CC
651 GGTACC
RsaI 144 GTAC GT^AC
652 GTAC
Sau3AI 324 GATC ^GATC
676 GATC
StuI 182 AGGCCT AGG^CCT
554 AGGCCT
TaqI 212 TCGA T^CGA
536 TCGA
Tsp45I 610 GTGAC ^GT[GC]AC
779 GTGAC
TthHB8I 212 TCGA T^CGA
536 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstNI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HapII
HinfI HpaII ItaI MaeIII MboII
MnlI MslI MspI MvaI MwoI
PleI Sau96I ScrFI SfaNI TseI
Tsp509I TspRI