Restriction map for
pFN21AE3906
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI AspEI AspI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BseRI
BsiEI BsiWI BsmBI BsoBI BspDI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KspI
MamI MluI MluNI MscI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 1184 GGTACC G^GTACC
AccI 853 GTAGAC GT^[AC][GT]AC
AccIII 1113 TCCGGA T^CCGGA
AcsI 593 AAATTC [GA]^AATT[CT]
AcyI 1297 GACGCC G[GA]^CG[CT]C
Alw44I 1022 GTGCAC G^TGCAC
AlwNI 1330 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1022 GTGCAC G^TGCAC
ApoI 593 AAATTC [GA]^AATT[CT]
Asp718I 1184 GGTACC G^GTACC
BamHI 1110 GGATCC G^GATCC
BanI 1184 GGTACC G^G[CT][GA]CC
BbsI 1363 GAAGAC GAAGAC(2/6)
BbvCI 196 GCTGAGG CCTCAGC(-5/-2)
BciVI 439 GGATAC GTATCC(6/5)
BcnI 216 CCGGG CC^[GC]GG
BglII 1282 AGATCT A^GATCT
BpuAI 1363 GAAGAC GAAGAC(2/6)
BsaAI 433 CACGTA [CT]AC^GT[GA]
BsaHI 1297 GACGCC G[GA]^CG[CT]C
BsaI 682 GGTCTC GGTCTC(1/5)
BseAI 1113 TCCGGA T^CCGGA
BsgI 707 GTGCAG GTGCAG(16/14)
BsmI 1239 GAATGC GAATGC(1/-1)
Bsp1407I 794 TGTACA T^GTACA
BspEI 1113 TCCGGA T^CCGGA
BspHI 1137 TCATGA T^CATGA
BspMI 1161 ACCTGC ACCTGC(4/8)
BsrGI 794 TGTACA T^GTACA
BssSI 817 CTCGTG CACGAG(-5/-1)
BstXI 1100 CCAAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 238 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1301 CCATGG C^C[GA][CT]GG
EaeI 841 CGGCCA [CT]^GGCC[GA]
EcoO109I 238 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1003 GATATC GAT^ATC
Hin1I 1297 GACGCC G[GA]^CG[CT]C
HincII 549 GTTGAC GT[CT]^[GA]AC
HindII 549 GTTGAC GT[CT]^[GA]AC
KpnI 1184 GGTACC GGTAC^C
MaeII 434 ACGT A^CGT
MfeI 1123 CAATTG C^AATTG
MroI 1113 TCCGGA T^CCGGA
MunI 1123 CAATTG C^AATTG
MwoI 1300 GCCATGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 216 CCGGG CC^[GC]GG
NcoI 1301 CCATGG C^CATGG
PflMI 339 CCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 238 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 798 CAGCTG CAG^CTG
RcaI 1137 TCATGA T^CATGA
SapI 566 GAAGAGC GCTCTTC(1/4)
SfcI 518 CTACAG C^T[GA][CT]AG
SspBI 794 TGTACA T^GTACA
Van91I 339 CCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 880 TCTAGA T^CTAGA
XcmI 335 CCATCCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 228 GCGG CCGC(-3/-1)
840 GCGG
AflIII 857 ACATGT A^C[GA][CT]GT
992 ACATGT
AlwI 1111 GATCC GGATC(4/5)
1110 GGATC
Asp700I 607 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1129 GAAACCCTTC
AspHI 430 GAGCAC G[AT]GC[AT]^C
1022 GTGCAC
BfaI 881 CTAG C^TAG
1294 CTAG
BmyI 430 GAGCAC G[AGT]GC[ACT]^C
1022 GTGCAC
BpmI 162 CTCCAG CTGGAG(16/14)
1286 CTCCAG
BsaWI 1113 TCCGGA [AT]^CCGG[AT]
1181 TCCGGT
BsiHKAI 430 GAGCAC G[AT]GC[AT]^C
1022 GTGCAC
BsmFI 238 GGGAC GGGAC(10/14)
751 GTCCC
Bsp1286I 430 GAGCAC G[AGT]GC[ACT]^C
1022 GTGCAC
BspLU11I 857 ACATGT A^CATGT
992 ACATGT
BsrDI 399 CATTGC GCAATG(2/0)
687 CATTGC
BstNI 235 CCAGG CC^[AT]GG
525 CCAGG
BstYI 1110 GGATCC [GA]^GATC[CT]
1282 AGATCT
EarI 140 GAAGAG CTCTTC(1/4)
566 GAAGAG
EcoRII 235 CCAGG ^CC[AT]GG
525 CCAGG
HgaI 813 GACGC GACGC(5/10)
1297 GACGC
HphI 348 GGTGA GGTGA(8/7)
967 GGTGA
Ksp632I 140 GAAGAG CTCTTC(1/4)
566 GAAGAG
MaeI 881 CTAG C^TAG
1294 CTAG
MflI 1110 GGATCC [GA]^GATC[CT]
1282 AGATCT
MspA1I 226 CAGCGG C[AC]G^C[GT]G
798 CAGCTG
MvaI 235 CCAGG CC^[AT]GG
525 CCAGG
NdeI 478 CATATG CA^TATG
1067 CATATG
PleI 331 GAGTC GAGTC(4/5)
877 GAGTC
SfaNI 1072 GATGC GCATC(5/9)
863 GCATC
TaqI 90 TCGA T^CGA
956 TCGA
TthHB8I 90 TCGA T^CGA
956 TCGA
XhoII 1110 GGATCC [GA]^GATC[CT]
1282 AGATCT
XmnI 607 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1129 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I Cac8I Cfr13I DdeI DpnII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MseI MslI MspI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI