Restriction map for
pFN21AE6115
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MluI
MroI MseI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 103 GTCTAC GT^[AC][GT]AC
AcsI 364 GAATTC [GA]^AATT[CT]
AocI 373 CCTGAGG CC^T[ACGT]AGG
ApoI 364 GAATTC [GA]^AATT[CT]
AvaII 113 GGACC G^G[AT]CC
BalI 413 TGGCCA TGG^CCA
BamHI 297 GGATCC G^GATCC
BanI 250 GGCACC G^G[CT][GA]CC
BbvI 288 GCTGC GCAGC(8/12)
BglI 41 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiYI 83 CCCTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 83 CCCTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrI 416 CCAGT ACTGG(1/-1)
BstEII 333 GGTGACC G^GT[ACGT]ACC
BstF5I 237 GGATG GGATG(2/0)
BstPI 333 GGTGACC G^GT[ACGT]ACC
BstYI 297 GGATCC [GA]^GATC[CT]
Bsu36I 373 CCTGAGG CC^T[ACGT]AGG
Cac8I 381 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 113 GGACC G^G[ACGT]CC
DsaI 109 CCATGG C^C[GA][CT]GG
EaeI 413 TGGCCA [CT]^GGCC[GA]
Eco81I 373 CCTGAGG CC^T[ACGT]AGG
EcoO65I 333 GGTGACC G^GT[ACGT]ACC
EcoRI 364 GAATTC G^AATTC
Fnu4HI 288 GCTGC GC^[ACGT]GC
FokI 237 GGATG GGATG(9/13)
HaeIII 414 GGCC GG^CC
ItaI 288 GCTGC GC^[ACGT]GC
MflI 297 GGATCC [GA]^GATC[CT]
MluNI 413 TGGCCA TGG^CCA
MscI 413 TGGCCA TGG^CCA
MslI 232 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 262 CAGCTG C[AC]G^C[GT]G
MwoI 41 GCCTGTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 109 CCATGG C^CATGG
NspI 291 GCATGT [GA]CATG^[CT]
PstI 407 CTGCAG CTGCA^G
PvuII 262 CAGCTG CAG^CTG
Sau96I 113 GGACC G^G[ACGT]CC
SfcI 407 CTGCAG C^T[GA][CT]AG
SmlI 244 CTCAAG C^T[CT][GA]AG
TseI 288 GCTGC G^C[AT]GC
Tsp509I 365 AATT ^AATT
XcmI 224 CCACAAAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 297 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwI 298 GATCC GGATC(4/5)
297 GGATC
BmyI 249 GGGCAC G[AGT]GC[ACT]^C
419 GTGCCC
BpmI 429 CTCCAG CTGGAG(16/14)
71 CTGGAG
BseRI 16 GAGGAG GAGGAG(10/8)
74 GAGGAG
BsgI 274 CTGCAC GTGCAG(16/14)
379 GTGCAG
Bsp1286I 249 GGGCAC G[AGT]GC[ACT]^C
419 GTGCCC
DpnII 241 GATC ^GATC
298 GATC
EcoT14I 109 CCATGG C^C[AT][AT]GG
212 CCTTGG
MboI 241 GATC ^GATC
298 GATC
MboII 64 GAAGA GAAGA(8/7)
396 GAAGA
NdeII 241 GATC ^GATC
298 GATC
Sau3AI 241 GATC ^GATC
298 GATC
SfaNI 67 GATGC GCATC(5/9)
220 GATGC
StyI 109 CCATGG C^C[AT][AT]GG
212 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BsaJI BssKI BstNI DdeI
EcoRII HphI MaeIII MnlI MvaI
NlaIII NlaIV ScrFI Tsp45I TspRI